ID   SSUD_VARPS              Reviewed;         389 AA.
AC   C5CNW9;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Alkanesulfonate monooxygenase {ECO:0000255|HAMAP-Rule:MF_01229};
DE            EC=1.14.14.5 {ECO:0000255|HAMAP-Rule:MF_01229};
DE   AltName: Full=FMNH2-dependent aliphatic sulfonate monooxygenase {ECO:0000255|HAMAP-Rule:MF_01229};
GN   Name=ssuD {ECO:0000255|HAMAP-Rule:MF_01229}; OrderedLocusNames=Vapar_2976;
OS   Variovorax paradoxus (strain S110).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Variovorax.
OX   NCBI_TaxID=543728;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S110;
RX   PubMed=21183664; DOI=10.1128/jb.00925-10;
RA   Han J.I., Choi H.K., Lee S.W., Orwin P.M., Kim J., Laroe S.L., Kim T.G.,
RA   O'Neil J., Leadbetter J.R., Lee S.Y., Hur C.G., Spain J.C.,
RA   Ovchinnikova G., Goodwin L., Han C.;
RT   "Complete genome sequence of the metabolically versatile plant growth-
RT   promoting endophyte, Variovorax paradoxus S110.";
RL   J. Bacteriol. 193:1183-1190(2011).
CC   -!- FUNCTION: Catalyzes the desulfonation of aliphatic sulfonates.
CC       {ECO:0000255|HAMAP-Rule:MF_01229}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + 2 H(+) +
CC         H2O + sulfite; Xref=Rhea:RHEA:23064, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17359,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:134249; EC=1.14.14.5; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01229};
CC   -!- SIMILARITY: Belongs to the SsuD family. {ECO:0000255|HAMAP-
CC       Rule:MF_01229}.
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DR   EMBL; CP001635; ACS19596.1; -; Genomic_DNA.
DR   RefSeq; WP_012748077.1; NC_012791.1.
DR   AlphaFoldDB; C5CNW9; -.
DR   SMR; C5CNW9; -.
DR   STRING; 543728.Vapar_2976; -.
DR   EnsemblBacteria; ACS19596; ACS19596; Vapar_2976.
DR   GeneID; 45057244; -.
DR   KEGG; vap:Vapar_2976; -.
DR   eggNOG; COG2141; Bacteria.
DR   HOGENOM; CLU_027853_1_0_4; -.
DR   OMA; NIFWFLP; -.
DR   OrthoDB; 919913at2; -.
DR   GO; GO:0008726; F:alkanesulfonate monooxygenase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.30; -; 1.
DR   HAMAP; MF_01229; Alkanesulf_monooxygen; 1.
DR   InterPro; IPR019911; Alkanesulphonate_mOase_FMN-dep.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   TIGRFAMs; TIGR03565; alk_sulf_monoox; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; Monooxygenase; Oxidoreductase.
FT   CHAIN           1..389
FT                   /note="Alkanesulfonate monooxygenase"
FT                   /id="PRO_1000214024"
SQ   SEQUENCE   389 AA;  42241 MW;  8F09E02CD519743A CRC64;
     MQIFWFLPTH GDSRYLGTSE GARPIDLAYL QQIAGAADQL GYEGVLIPTG RSCEDPWVIA
     SSLIGATKNL KFLVAVRPGL HQPSLAARMA ATFDRLSGGR LLVNLVTGGD QSELEGDGVY
     LDHAQRYEQS AEFIRIWREI LARSHSGQAF DYEGRHLSVK GAKLLYPPVQ EPYPPVWFGG
     SSAAAHELAA EQVDAYLTWG EPPAEVARKI ADVRNRAARK GRTVEFGIRL HVIVRETEDA
     AWKAAEELIS RVDDETVIRA QAAFARMDSE GQRRMAALHA GGARRSRAEL EISPNLWAGV
     GLVRGGAGTA LVGDPKTVAA RIEEYAALGL DKFILSGYPH LEEAYRFAEL VFPLLSRKAQ
     SRLAGGSLSG PFGEVVANLD APSRLASQS