SSX4_HUMAN
ID SSX4_HUMAN Reviewed; 188 AA.
AC O60224; A8MYD4; B2RPE3; Q3SYD4; Q5JQZ0; Q9UJU9;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Protein SSX4;
DE AltName: Full=Cancer/testis antigen 5.4;
DE Short=CT5.4;
GN Name=SSX4; Synonyms=SSX4A;
GN and
GN Name=SSX4B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=9378559;
RX DOI=10.1002/(sici)1097-0215(19970917)72:6<965::aid-ijc8>3.0.co;2-n;
RA Gure A.O., Tuereci O., Sahin U., Tsang S., Scanlan M.J., Jager E.,
RA Knuth A., Pfreundschuh M., Old L.J., Chen Y.-T.;
RT "SSX: a multigene family with several members transcribed in normal testis
RT and human cancer.";
RL Int. J. Cancer 72:965-971(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Could act as a modulator of transcription.
CC -!- INTERACTION:
CC O60224; Q9NWQ9: C14orf119; NbExp=3; IntAct=EBI-745513, EBI-725606;
CC O60224; Q96Q35-2: FLACC1; NbExp=3; IntAct=EBI-745513, EBI-11533409;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O60224-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O60224-2; Sequence=VSP_054114, VSP_054115;
CC -!- SIMILARITY: Belongs to the SSX family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI03865.1; Type=Miscellaneous discrepancy; Note=Several sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; U90841; AAC05820.1; -; mRNA.
DR EMBL; AF196972; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL606490; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC005325; AAH05325.1; -; mRNA.
DR EMBL; BC103864; AAI03865.1; ALT_SEQ; mRNA.
DR EMBL; BC137394; AAI37395.1; -; mRNA.
DR EMBL; BC137395; AAI37396.1; -; mRNA.
DR CCDS; CCDS35240.1; -. [O60224-1]
DR CCDS; CCDS35241.1; -. [O60224-1]
DR CCDS; CCDS43934.1; -. [O60224-2]
DR CCDS; CCDS43935.1; -. [O60224-2]
DR RefSeq; NP_001030004.1; NM_001034832.3. [O60224-1]
DR RefSeq; NP_001035702.1; NM_001040612.2. [O60224-2]
DR RefSeq; NP_005627.1; NM_005636.3. [O60224-1]
DR RefSeq; NP_783856.1; NM_175729.1. [O60224-2]
DR RefSeq; XP_016885102.1; XM_017029613.1. [O60224-1]
DR RefSeq; XP_016885248.1; XM_017029759.1. [O60224-1]
DR AlphaFoldDB; O60224; -.
DR SMR; O60224; -.
DR BioGRID; 112637; 10.
DR BioGRID; 139210; 2.
DR IntAct; O60224; 7.
DR MINT; O60224; -.
DR STRING; 9606.ENSP00000469011; -.
DR iPTMnet; O60224; -.
DR PhosphoSitePlus; O60224; -.
DR BioMuta; SSX4; -.
DR MassIVE; O60224; -.
DR PaxDb; O60224; -.
DR PeptideAtlas; O60224; -.
DR PRIDE; O60224; -.
DR ProteomicsDB; 2393; -.
DR ProteomicsDB; 49249; -. [O60224-1]
DR Antibodypedia; 72375; 175 antibodies from 22 providers.
DR Antibodypedia; 76700; 25 antibodies from 9 providers.
DR DNASU; 6759; -.
DR Ensembl; ENST00000595235.6; ENSP00000469394.1; ENSG00000269791.6. [O60224-1]
DR Ensembl; ENST00000595689.3; ENSP00000469011.1; ENSG00000268009.6. [O60224-1]
DR Ensembl; ENST00000619890.1; ENSP00000481765.1; ENSG00000269791.6. [O60224-2]
DR Ensembl; ENST00000620320.4; ENSP00000483262.1; ENSG00000268009.6. [O60224-2]
DR GeneID; 548313; -.
DR GeneID; 6759; -.
DR KEGG; hsa:548313; -.
DR KEGG; hsa:6759; -.
DR MANE-Select; ENST00000595235.6; ENSP00000469394.1; NM_001034832.5; NP_001030004.1.
DR MANE-Select; ENST00000595689.3; ENSP00000469011.1; NM_005636.4; NP_005627.1.
DR UCSC; uc004djf.3; human. [O60224-1]
DR CTD; 548313; -.
DR CTD; 6759; -.
DR DisGeNET; 548313; -.
DR DisGeNET; 6759; -.
DR GeneCards; SSX4; -.
DR GeneCards; SSX4B; -.
DR HGNC; HGNC:11338; SSX4.
DR HGNC; HGNC:16880; SSX4B.
DR HPA; ENSG00000268009; Tissue enriched (testis).
DR HPA; ENSG00000269791; Tissue enriched (testis).
DR MIM; 300326; gene.
DR neXtProt; NX_O60224; -.
DR OpenTargets; ENSG00000268009; -.
DR OpenTargets; ENSG00000269791; -.
DR PharmGKB; PA36162; -.
DR VEuPathDB; HostDB:ENSG00000268009; -.
DR VEuPathDB; HostDB:ENSG00000269791; -.
DR eggNOG; ENOG502RU1A; Eukaryota.
DR GeneTree; ENSGT00390000012484; -.
DR HOGENOM; CLU_097196_1_0_1; -.
DR InParanoid; O60224; -.
DR OMA; FHGNDFD; -.
DR OrthoDB; 1270676at2759; -.
DR PhylomeDB; O60224; -.
DR TreeFam; TF338517; -.
DR PathwayCommons; O60224; -.
DR SignaLink; O60224; -.
DR BioGRID-ORCS; 548313; 8 hits in 606 CRISPR screens.
DR BioGRID-ORCS; 6759; 208 hits in 590 CRISPR screens.
DR ChiTaRS; SSX4; human.
DR ChiTaRS; SSX4B; human.
DR GeneWiki; SSX4_(gene); -.
DR Pharos; O60224; Tbio.
DR PRO; PR:O60224; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; O60224; protein.
DR Bgee; ENSG00000268009; Expressed in right testis and 18 other tissues.
DR Genevisible; O60224; HS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd07765; KRAB_A-box; 1.
DR InterPro; IPR003655; aKRAB.
DR InterPro; IPR001909; KRAB.
DR InterPro; IPR036051; KRAB_dom_sf.
DR InterPro; IPR028804; SSX.
DR InterPro; IPR019041; SSXRD_motif.
DR PANTHER; PTHR14112; PTHR14112; 1.
DR Pfam; PF09514; SSXRD; 1.
DR SMART; SM00349; KRAB; 1.
DR SUPFAM; SSF109640; SSF109640; 1.
DR PROSITE; PS50806; KRAB_RELATED; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..188
FT /note="Protein SSX4"
FT /id="PRO_0000181831"
FT DOMAIN 20..83
FT /note="KRAB-related"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00120"
FT REGION 116..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 137..153
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 111..153
FT /note="IMPKKPAEEENGLKEVPEASGPQNDGKQLCPPGNPSTLEKINK -> DPKGG
FT NMPGPTDCVRESSWWFMKRSATLRKMTSNSPRGYDTCP (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_054114"
FT VAR_SEQ 154..188
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_054115"
SQ SEQUENCE 188 AA; 21858 MW; 4ACA2A8737507AE5 CRC64;
MNGDDAFARR PRDDAQISEK LRKAFDDIAK YFSKKEWEKM KSSEKIVYVY MKLNYEVMTK
LGFKVTLPPF MRSKRAADFH GNDFGNDRNH RNQVERPQMT FGSLQRIFPK IMPKKPAEEE
NGLKEVPEAS GPQNDGKQLC PPGNPSTLEK INKTSGPKRG KHAWTHRLRE RKQLVVYEEI
SDPEEDDE
