ST217_CAEEL
ID ST217_CAEEL Reviewed; 344 AA.
AC Q9XX85;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=G-protein coupled receptor str-217 {ECO:0000305};
GN Name=str-217 {ECO:0000312|WormBase:Y102A5C.28};
GN ORFNames=Y102A5C.28 {ECO:0000312|WormBase:Y102A5C.28};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP TISSUE SPECIFICITY, POLYMORPHISM, DISRUPTION PHENOTYPE, ROLE IN RESPONSE TO
RP DEET, AND MUTAGENESIS OF PRO-314.
RX PubMed=30258230; DOI=10.1038/s41586-018-0546-8;
RA Dennis E.J., Dobosiewicz M., Jin X., Duvall L.B., Hartman P.S.,
RA Bargmann C.I., Vosshall L.B.;
RT "A natural variant and engineered mutation in a GPCR promote DEET
RT resistance in C. elegans.";
RL Nature 562:119-123(2018).
CC -!- FUNCTION: Probable G-protein coupled receptor. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in the ADL chemosensory neurons.
CC {ECO:0000269|PubMed:30258230}.
CC -!- POLYMORPHISM: The naturally occurring CB4856 strain isolated in Hawaii
CC carries a deletion of str-217 and is resistant to N,N-diethyl-meta-
CC toluamide (DEET). {ECO:0000269|PubMed:30258230}.
CC -!- DISRUPTION PHENOTYPE: Lack of sensitivity of ADL neurons to N,N-
CC diethyl-meta-toluamide (DEET) with no effect on average pause length of
CC worms following DEET exposure in contrast to wild-type worms which show
CC a marked increase (PubMed:30258230). Does not affect AWC neuron
CC activity in response to DEET (PubMed:30258230).
CC {ECO:0000269|PubMed:30258230}.
CC -!- MISCELLANEOUS: Mediates sensitivity to N,N-diethyl-meta-toluamide
CC (DEET), the most widely used insect repellent worldwide.
CC {ECO:0000269|PubMed:30258230}.
CC -!- SIMILARITY: Belongs to the nematode receptor-like protein str family.
CC {ECO:0000305}.
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DR EMBL; BX284605; CAA20950.2; -; Genomic_DNA.
DR PIR; T26348; T26348.
DR RefSeq; NP_507300.1; NM_074899.1.
DR AlphaFoldDB; Q9XX85; -.
DR STRING; 6239.Y102A5C.28; -.
DR PaxDb; Q9XX85; -.
DR EnsemblMetazoa; Y102A5C.28.1; Y102A5C.28.1; WBGene00006249.
DR GeneID; 190851; -.
DR KEGG; cel:CELE_Y102A5C.28; -.
DR UCSC; Y102A5C.28; c. elegans.
DR CTD; 190851; -.
DR WormBase; Y102A5C.28; CE25158; WBGene00006249; str-217.
DR eggNOG; ENOG502TG6X; Eukaryota.
DR GeneTree; ENSGT00970000196029; -.
DR HOGENOM; CLU_036335_2_0_1; -.
DR InParanoid; Q9XX85; -.
DR OMA; PAQMGNY; -.
DR OrthoDB; 1350620at2759; -.
DR PhylomeDB; Q9XX85; -.
DR PRO; PR:Q9XX85; -.
DR Proteomes; UP000001940; Chromosome V.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0038022; F:G protein-coupled olfactory receptor activity; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0042048; P:olfactory behavior; IBA:GO_Central.
DR GO; GO:0017085; P:response to insecticide; IMP:UniProtKB.
DR InterPro; IPR019428; 7TM_GPCR_serpentine_rcpt_Str.
DR Pfam; PF10326; 7TM_GPCR_Str; 1.
PE 1: Evidence at protein level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..344
FT /note="G-protein coupled receptor str-217"
FT /id="PRO_0000449905"
FT TOPO_DOM 1..10
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 11..31
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..39
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 40..60
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 61..88
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 89..109
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 129..149
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..195
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 196..216
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 217..256
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 257..277
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 278..279
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 280..300
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 301..344
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT CARBOHYD 170
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT MUTAGEN 314
FT /note="P->S: In LBV003; confers resistance to N,N-diethyl-
FT meta-toluamide (DEET)."
FT /evidence="ECO:0000269|PubMed:30258230"
SQ SEQUENCE 344 AA; 38992 MW; 0AC1FA61B9984B6A CRC64;
MLLFQKTLSR VAAPISVAAN LILILLIIFK SPAQMGNYKY LLIGLSIFEM SYAVLDVVSE
TTVLSIKKSF VVVVPYKDRS FGQETAMDIN LIYCGFFGFS MGMFVVIFAY RSFLTTGNTI
LRKFEGFKII SWFAYPLFYA IVWILVAWGP LASFPEMDIV VRPFLLDELN MTVDEVAYTG
RLFYSTIDNS LRYSAILTGV LQWVLTASSL FLVIFFGLRC YFHYGKLVQL TDVQSIRLRQ
LQNQLFLALV CQATVPLILM HIPVTILYTC CVLNIVFNPF SVATTIALFP AIDPLPTIFI
VKNYRVALFE FVCPSCLCWS ETLKHMGSNR ITSYRSNTVN ALSM
