ID   ST4A1_RAT               Reviewed;         284 AA.
AC   P63047; O88872; Q91XS5; Q9CWY7; Q9DC97;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   31-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Sulfotransferase 4A1;
DE            Short=ST4A1;
DE            EC=2.8.2.-;
DE   AltName: Full=Brain sulfotransferase-like protein;
DE            Short=rBR-STL;
DE   AltName: Full=Nervous system sulfotransferase;
DE            Short=NST;
GN   Name=Sult4a1; Synonyms=Sultx3;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=Sprague-Dawley; TISSUE=Brain;
RX   PubMed=10698717; DOI=10.1042/bj3460857;
RA   Falany C.N., Xie X., Wang J., Ferrer J., Falany J.L.;
RT   "Molecular cloning and expression of novel sulphotransferase-like cDNAs
RT   from human and rat brain.";
RL   Biochem. J. 346:857-864(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 125-284.
RC   TISSUE=Brain;
RA   Martin S.C., Farb D.H.;
RT   "Molecular identification of a rat nervous system cytoplasmic
RT   sulfotransferase, NST.";
RL   Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-8 AND THR-11, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: Atypical sulfotransferase family member with very low
CC       affinity for 3'-phospho-5'-adenylyl sulfate (PAPS) and very low
CC       catalytic activity towards L-triiodothyronine, thyroxine, estrone, p-
CC       nitrophenol, 2-naphthylamine, and 2-beta-naphthol. May have a role in
CC       the metabolism of drugs and neurotransmitters in the CNS (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10698717}.
CC   -!- TISSUE SPECIFICITY: Expressed in the brain, not detected in the liver,
CC       kidney, spleen, heart, small intestine or testis.
CC       {ECO:0000269|PubMed:10698717}.
CC   -!- DEVELOPMENTAL STAGE: Expressed at low levels in brains of 1-day old
CC       animals but increase to adult levels from 7-day old animals and remain
CC       at that level in adults. {ECO:0000269|PubMed:10698717}.
CC   -!- SIMILARITY: Belongs to the sulfotransferase 1 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF188699; AAF61198.1; -; mRNA.
DR   EMBL; AF176343; AAK64596.1; -; mRNA.
DR   RefSeq; NP_113829.1; NM_031641.1.
DR   AlphaFoldDB; P63047; -.
DR   SMR; P63047; -.
DR   STRING; 10116.ENSRNOP00000066760; -.
DR   iPTMnet; P63047; -.
DR   PhosphoSitePlus; P63047; -.
DR   PaxDb; P63047; -.
DR   PRIDE; P63047; -.
DR   Ensembl; ENSRNOT00000075099; ENSRNOP00000066760; ENSRNOG00000046975.
DR   GeneID; 58953; -.
DR   KEGG; rno:58953; -.
DR   CTD; 25830; -.
DR   RGD; 69292; Sult4a1.
DR   eggNOG; KOG1584; Eukaryota.
DR   GeneTree; ENSGT00940000158662; -.
DR   HOGENOM; CLU_027239_1_1_1; -.
DR   InParanoid; P63047; -.
DR   OMA; MVESCHQ; -.
DR   OrthoDB; 780670at2759; -.
DR   PhylomeDB; P63047; -.
DR   Reactome; R-RNO-156584; Cytosolic sulfonation of small molecules.
DR   PRO; PR:P63047; -.
DR   Proteomes; UP000002494; Chromosome 7.
DR   Bgee; ENSRNOG00000046975; Expressed in frontal cortex and 11 other tissues.
DR   Genevisible; P63047; RN.
DR   GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR   GO; GO:0005829; C:cytosol; ISO:RGD.
DR   GO; GO:0005739; C:mitochondrion; ISO:RGD.
DR   GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR   GO; GO:0008146; F:sulfotransferase activity; ISO:RGD.
DR   GO; GO:0140059; P:dendrite arborization; IMP:UniProtKB.
DR   GO; GO:0008202; P:steroid metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0051923; P:sulfation; IBA:GO_Central.
DR   GO; GO:0006790; P:sulfur compound metabolic process; ISO:RGD.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000863; Sulfotransferase_dom.
DR   Pfam; PF00685; Sulfotransfer_1; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Lipid metabolism; Phosphoprotein; Reference proteome;
KW   Steroid metabolism; Transferase.
FT   CHAIN           1..284
FT                   /note="Sulfotransferase 4A1"
FT                   /id="PRO_0000085169"
FT   MOD_RES         8
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         11
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         205
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BR01"
SQ   SEQUENCE   284 AA;  33054 MW;  FCAE940F7219E6FC CRC64;
     MAESEAETPG TPGEFESKYF EFHGVRLPPF CRGKMEDIAD FPVRPSDVWI VTYPKSGTSL
     LQEVVYLVSQ GADPDEIGLM NIDEQLPVLE YPQPGLDIIK ELTSPRLIKS HLPYRFLPSD
     LHNGDSKVIY MARNPKDLVV SYYQFHRSLR TMSYRGTFQE FCRRFMNDKL GYGSWFEHVQ
     EFWEHRMDAN VLFLKYEDMH RDLVTMVEQL ARFLGVSCDK AQLESLIEHC HQLVDQCCNA
     EALPVGRGRV GLWKDIFTVS MNEKFDLVYK QKMGKCDLTF DFYL