STA31_XENLA
ID STA31_XENLA Reviewed; 769 AA.
AC Q9PVX8;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Signal transducer and activator of transcription 3.1;
DE AltName: Full=Protein Stat3 {ECO:0000250|UniProtKB:P40763};
DE Short=Xstat3 {ECO:0000303|PubMed:10642787};
GN Name=stat3.1; Synonyms=stat3 {ECO:0000303|PubMed:10642787};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAA86061.1}
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo {ECO:0000269|PubMed:10642787};
RX PubMed=10642787; DOI=10.1006/dbio.1999.9518;
RA Nishinakamura R., Matsumoto Y., Matsuda T., Ariizumi T., Heike T.,
RA Asashima M., Yokota T.;
RT "Activation of Stat3 by cytokine receptor gp130 ventralizes Xenopus embryos
RT independent of BMP-4.";
RL Dev. Biol. 216:481-490(1999).
RN [2] {ECO:0000312|EMBL:AAI69718.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo {ECO:0000312|EMBL:AAI69718.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP FUNCTION, INTERACTION WITH NLK.2, PHOSPHORYLATION AT SER-728, AND
RP MUTAGENESIS OF 662-ALA--ASN-664; TYR-706 AND SER-728.
RX PubMed=15004007; DOI=10.1101/gad.1166904;
RA Ohkawara B., Shirakabe K., Hyodo-Miura J., Matsuo R., Ueno N.,
RA Matsumoto K., Shibuya H.;
RT "Role of the TAK1-NLK-STAT3 pathway in TGF-beta-mediated mesoderm
RT induction.";
RL Genes Dev. 18:381-386(2004).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=18710660; DOI=10.1016/j.ydbio.2008.07.026;
RA Nichane M., Ren X., Souopgui J., Bellefroid E.J.;
RT "Hairy2 functions through both DNA-binding and non DNA-binding mechanisms
RT at the neural plate border in Xenopus.";
RL Dev. Biol. 322:368-380(2008).
CC -!- FUNCTION: Transcription factor that binds to target promoter sequences
CC and activates transcription upon il6st/gp130 stimulation. Mediates
CC ventralization of embryos, at least in part via inhibition of smad2
CC signaling. Required for hairy2 to induce dll1/delta1 and promote neural
CC crest cell proliferation and differentiation. Involved in TGFbeta-
CC mediated mesoderm induction in early embryos, acting downstream of
CC map3k7/tak1 and nlk.2. {ECO:0000269|PubMed:15004007,
CC ECO:0000269|PubMed:18710660}.
CC -!- SUBUNIT: Forms a homodimer or a heterodimer with a related family
CC member, such as stat1 (By similarity). Interacts with nlk.2.
CC {ECO:0000250|UniProtKB:P40763, ECO:0000269|PubMed:15004007}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P40763}. Nucleus
CC {ECO:0000250|UniProtKB:P40763}. Note=Shuttles between the nucleus and
CC the cytoplasm. Constitutive nuclear presence is independent of tyrosine
CC phosphorylation (By similarity). {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Expressed from the one-cell stage throughout
CC embryogenesis (at protein level). Expressed fairly ubiquitously up to
CC the gastrula stage, then restricted mainly to the nervous system later
CC in development. {ECO:0000269|PubMed:10642787}.
CC -!- PTM: Phosphorylation of both tyrosine and serine residues, together
CC with dimerization, is required for mesoderm induction.
CC {ECO:0000269|PubMed:15004007}.
CC -!- MISCELLANEOUS: Involved in the gp130-mediated signaling pathway.
CC {ECO:0000269|PubMed:10642787}.
CC -!- SIMILARITY: Belongs to the transcription factor STAT family.
CC {ECO:0000255}.
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DR EMBL; AB017701; BAA86061.1; -; mRNA.
DR EMBL; BC169718; AAI69718.1; -; mRNA.
DR EMBL; BC169720; AAI69720.1; -; mRNA.
DR RefSeq; NP_001090202.1; NM_001096733.1.
DR AlphaFoldDB; Q9PVX8; -.
DR SMR; Q9PVX8; -.
DR iPTMnet; Q9PVX8; -.
DR GeneID; 779100; -.
DR KEGG; xla:779100; -.
DR CTD; 779100; -.
DR Xenbase; XB-GENE-865436; stat3.1.L.
DR OMA; RCLWEEN; -.
DR OrthoDB; 327469at2759; -.
DR Proteomes; UP000186698; Chromosome 9_10L.
DR Bgee; 779100; Expressed in egg cell and 19 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0046983; F:protein dimerization activity; ISS:UniProtKB.
DR GO; GO:0019901; F:protein kinase binding; IPI:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0042755; P:eating behavior; ISS:UniProtKB.
DR GO; GO:0001754; P:eye photoreceptor cell differentiation; ISS:UniProtKB.
DR GO; GO:0042593; P:glucose homeostasis; ISS:UniProtKB.
DR GO; GO:0060397; P:growth hormone receptor signaling pathway via JAK-STAT; ISS:UniProtKB.
DR GO; GO:0001707; P:mesoderm formation; IMP:UniProtKB.
DR GO; GO:0014033; P:neural crest cell differentiation; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0019953; P:sexual reproduction; ISS:UniProtKB.
DR GO; GO:0001659; P:temperature homeostasis; ISS:UniProtKB.
DR CDD; cd10374; SH2_STAT3; 1.
DR Gene3D; 1.10.532.10; -; 1.
DR Gene3D; 2.60.40.630; -; 1.
DR Gene3D; 3.30.505.10; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR000980; SH2.
DR InterPro; IPR036860; SH2_dom_sf.
DR InterPro; IPR001217; STAT.
DR InterPro; IPR035855; STAT3_SH2.
DR InterPro; IPR036535; STAT_N_sf.
DR InterPro; IPR013800; STAT_TF_alpha.
DR InterPro; IPR015988; STAT_TF_coiled-coil.
DR InterPro; IPR013801; STAT_TF_DNA-bd.
DR InterPro; IPR012345; STAT_TF_DNA-bd_N.
DR InterPro; IPR013799; STAT_TF_prot_interaction.
DR PANTHER; PTHR11801; PTHR11801; 1.
DR Pfam; PF00017; SH2; 1.
DR Pfam; PF01017; STAT_alpha; 1.
DR Pfam; PF02864; STAT_bind; 1.
DR Pfam; PF02865; STAT_int; 1.
DR SMART; SM00964; STAT_int; 1.
DR SUPFAM; SSF47655; SSF47655; 1.
DR SUPFAM; SSF48092; SSF48092; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR SUPFAM; SSF55550; SSF55550; 1.
DR PROSITE; PS50001; SH2; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; Developmental protein; DNA-binding; Nucleus;
KW Phosphoprotein; Reference proteome; SH2 domain; Transcription;
KW Transcription regulation.
FT CHAIN 1..769
FT /note="Signal transducer and activator of transcription
FT 3.1"
FT /id="PRO_0000370237"
FT DOMAIN 580..670
FT /note="SH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT MOTIF 150..162
FT /note="Essential for nuclear import"
FT /evidence="ECO:0000250"
FT MOD_RES 728
FT /note="Phosphoserine; by NLK"
FT /evidence="ECO:0000269|PubMed:15004007"
FT MUTAGEN 662..664
FT /note="ATN->CTC: Does not induce t/bra expression; when
FT associated A-728. Induces t/bra expression; when associated
FT with E-728."
FT /evidence="ECO:0000269|PubMed:15004007"
FT MUTAGEN 706
FT /note="Y->F: Inhibits t/bra expression and mesoderm
FT induction."
FT /evidence="ECO:0000269|PubMed:15004007"
FT MUTAGEN 728
FT /note="S->A: Inhibits t/bra expression and mesoderm
FT induction."
FT /evidence="ECO:0000269|PubMed:15004007"
FT MUTAGEN 728
FT /note="S->E: Induces t/bra expression; when associated with
FT 662-CTC-664."
FT /evidence="ECO:0000269|PubMed:15004007"
SQ SEQUENCE 769 AA; 87975 MW; 0905C03263303069 CRC64;
MAQWNQLQQL DTRYLEQLHQ LYSDSFPMEL RQFLAPWIES QDWAFAASKE SHATLVFHNL
LGEIDQQYSR FLQESNVLYQ HNLRRIKQFL QSRYLEKPME IARIVARCLW EEGRLLQTAA
AAAQQGGPAS HPNAAVVTEK QQMLEQHLQD VRKKVQDLEQ KMKVVENLQD DFDFNYKTLK
SQSDLSELNG NNQSVTRQKM QQLEQMLTAL DQLRRTIISD LASLLSAMEY VQKTLTDEEL
ADWKRRQQIA CIGGPPNICL DRLENWITSL AESQLQIRQQ IKKLEELQQK VSYKGDPIVQ
HRPMLEERIV ELFRNLMKSA FVVERQPCMP MHPDRPLVIK TGVQFTNKVR LLVKFPELNY
QLKIKVCIDK DSGDVAALRG SRKFNILGTN TKVMNMEESN NGSLSAEFKH LTLREQRCGN
GGRANCDASL IVTEELHLIT FETEVYHQGL KIDLETHSLP VVVISNICQM PNAWASILWY
NMLTNNPKNV NFFTKPPIGT WDQVAEVLSW QFSSTTKRGL SIEQLTTLAE KLLGPGVNYS
GCQITWAKFC KENMAGKGFS FWVWLDNIID LVKKYILALW NEGYIMGFIS KERERAILST
KPPGTFLLRF SESSKEGGIT FTWVEKDISG KTQIQSVEPY TKQQLNNMSF AEIIMGYKIM
DATNILVSPL VYLYPDIPKE EAFGKYCRPE SQEHQEPTDP GSTAPYLKTK FICVTPTTCS
STLDLPMSPR TLDSLMQFPG EGADSSAGNQ FETLTFDMEL TSECASSPM
