STA5A_SHEEP
ID STA5A_SHEEP Reviewed; 794 AA.
AC P42231;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 25-MAY-2022, entry version 139.
DE RecName: Full=Signal transducer and activator of transcription 5A;
DE AltName: Full=Mammary gland factor;
GN Name=STAT5A; Synonyms=MGF, STAT5;
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Mammary gland;
RX PubMed=7514531; DOI=10.1002/j.1460-2075.1994.tb06495.x;
RA Wakao H., Gouilleux F., Groner B.;
RT "Mammary gland factor (MGF) is a novel member of the cytokine regulated
RT transcription factor gene family and confers the prolactin response.";
RL EMBO J. 13:2182-2191(1994).
RN [2]
RP ERRATUM OF PUBMED:7514531, AND SEQUENCE REVISION.
RX PubMed=7882987; DOI=10.1002/j.1460-2075.1995.tb07064.x;
RA Wakao H., Gouilleux F., Groner B.;
RL EMBO J. 14:854-855(1995).
RN [3]
RP PARTIAL PROTEIN SEQUENCE.
RC TISSUE=Mammary gland;
RX PubMed=7537213; DOI=10.1002/j.1460-2075.1995.tb07126.x;
RA Azam M., Erdjument-Bromage H., Kreider B.L., Xia M., Quelle F., Basu R.,
RA Saris C., Tempst P., Ihle J.N., Schindler C.;
RT "Interleukin-3 signals through multiple isoforms of Stat5.";
RL EMBO J. 14:1402-1411(1995).
CC -!- FUNCTION: Carries out a dual function: signal transduction and
CC activation of transcription. Mediates cellular responses to the
CC cytokine KITLG/SCF and other growth factors. May mediate cellular
CC responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS
CC element and activates PRL-induced transcription. Regulates the
CC expression of milk proteins during lactation.
CC -!- SUBUNIT: Forms a homodimer or a heterodimer with a related family
CC member. Binds NR3C1. Interacts with NCOA1 and SOCS7. Interacts with
CC ERBB4 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocated into the
CC nucleus in response to phosphorylation.
CC -!- TISSUE SPECIFICITY: Found in mammary gland and, in lesser extent, in
CC ovary, thymus, spleen, kidney, lung, muscle and adrenal gland.
CC -!- INDUCTION: By prolactin.
CC -!- PTM: ISGylated. {ECO:0000250|UniProtKB:P42230}.
CC -!- PTM: Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7,
CC IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO (By similarity). Activated KIT
CC promotes phosphorylation on tyrosine residues and subsequent
CC translocation to the nucleus (By similarity). Tyrosine phosphorylated
CC in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4
CC (By similarity). Tyrosine phosphorylation is required for DNA-binding
CC activity and dimerization. Serine phosphorylation is also required for
CC maximal transcriptional activity (By similarity). Tyrosine
CC phosphorylated in response to signaling via activated FLT3; wild-type
CC FLT3 results in much weaker phosphorylation than constitutively
CC activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 at
CC Tyr-694 (By similarity). {ECO:0000250|UniProtKB:P40763,
CC ECO:0000250|UniProtKB:P42229, ECO:0000250|UniProtKB:P42230,
CC ECO:0000250|UniProtKB:Q62771}.
CC -!- SIMILARITY: Belongs to the transcription factor STAT family.
CC {ECO:0000305}.
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DR EMBL; X78428; CAA55191.1; -; mRNA.
DR PIR; S55527; S55527.
DR RefSeq; NP_001009402.1; NM_001009402.1.
DR AlphaFoldDB; P42231; -.
DR SMR; P42231; -.
DR STRING; 9940.ENSOARP00000000859; -.
DR iPTMnet; P42231; -.
DR GeneID; 443419; -.
DR KEGG; oas:443419; -.
DR CTD; 6776; -.
DR eggNOG; KOG3667; Eukaryota.
DR OrthoDB; 327469at2759; -.
DR Proteomes; UP000002356; Unplaced.
DR GO; GO:0005737; C:cytoplasm; ISS:AgBase.
DR GO; GO:0005634; C:nucleus; ISS:AgBase.
DR GO; GO:0003677; F:DNA binding; ISS:AgBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0019221; P:cytokine-mediated signaling pathway; ISS:AgBase.
DR GO; GO:0046543; P:development of secondary female sexual characteristics; ISS:AgBase.
DR GO; GO:0007565; P:female pregnancy; ISS:AgBase.
DR GO; GO:0060397; P:growth hormone receptor signaling pathway via JAK-STAT; IEA:InterPro.
DR GO; GO:0007595; P:lactation; ISS:AgBase.
DR GO; GO:0019915; P:lipid storage; ISS:AgBase.
DR GO; GO:0001553; P:luteinization; ISS:AgBase.
DR GO; GO:0030879; P:mammary gland development; ISS:AgBase.
DR GO; GO:0001779; P:natural killer cell differentiation; ISS:AgBase.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISS:AgBase.
DR GO; GO:0045647; P:negative regulation of erythrocyte differentiation; ISS:AgBase.
DR GO; GO:0042104; P:positive regulation of activated T cell proliferation; ISS:AgBase.
DR GO; GO:0045579; P:positive regulation of B cell differentiation; ISS:AgBase.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISS:AgBase.
DR GO; GO:0050729; P:positive regulation of inflammatory response; ISS:AgBase.
DR GO; GO:0032743; P:positive regulation of interleukin-2 production; ISS:AgBase.
DR GO; GO:0045931; P:positive regulation of mitotic cell cycle; ISS:AgBase.
DR GO; GO:0040018; P:positive regulation of multicellular organism growth; ISS:AgBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:AgBase.
DR GO; GO:0007259; P:receptor signaling pathway via JAK-STAT; ISS:AgBase.
DR GO; GO:0030155; P:regulation of cell adhesion; ISS:AgBase.
DR GO; GO:0030856; P:regulation of epithelial cell differentiation; ISS:AgBase.
DR GO; GO:0019218; P:regulation of steroid metabolic process; ISS:AgBase.
DR GO; GO:0043029; P:T cell homeostasis; ISS:AgBase.
DR Gene3D; 1.10.532.10; -; 1.
DR Gene3D; 2.60.40.630; -; 1.
DR Gene3D; 3.30.505.10; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR000980; SH2.
DR InterPro; IPR036860; SH2_dom_sf.
DR InterPro; IPR001217; STAT.
DR InterPro; IPR035858; STAT5a/5b.
DR InterPro; IPR036535; STAT_N_sf.
DR InterPro; IPR013800; STAT_TF_alpha.
DR InterPro; IPR015988; STAT_TF_coiled-coil.
DR InterPro; IPR013801; STAT_TF_DNA-bd.
DR InterPro; IPR012345; STAT_TF_DNA-bd_N.
DR InterPro; IPR013799; STAT_TF_prot_interaction.
DR PANTHER; PTHR11801; PTHR11801; 1.
DR PANTHER; PTHR11801:SF47; PTHR11801:SF47; 1.
DR Pfam; PF00017; SH2; 1.
DR Pfam; PF01017; STAT_alpha; 1.
DR Pfam; PF02864; STAT_bind; 1.
DR Pfam; PF02865; STAT_int; 1.
DR SMART; SM00252; SH2; 1.
DR SMART; SM00964; STAT_int; 1.
DR SUPFAM; SSF47655; SSF47655; 1.
DR SUPFAM; SSF48092; SSF48092; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR SUPFAM; SSF55550; SSF55550; 1.
DR PROSITE; PS50001; SH2; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; Direct protein sequencing; DNA-binding; Lactation;
KW Nucleus; Phosphoprotein; Reference proteome; SH2 domain; Transcription;
KW Transcription regulation; Ubl conjugation.
FT CHAIN 1..794
FT /note="Signal transducer and activator of transcription 5A"
FT /id="PRO_0000182427"
FT DOMAIN 589..686
FT /note="SH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT REGION 765..794
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 90
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P42229"
FT MOD_RES 129
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P42229"
FT MOD_RES 682
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P42229"
FT MOD_RES 694
FT /note="Phosphotyrosine; by JAK2"
FT /evidence="ECO:0000250|UniProtKB:P51692"
SQ SEQUENCE 794 AA; 90917 MW; E69A4EC826AF60BE CRC64;
MAGWIQAQQL QGDALRQMQV LYGQHFPIEV RHYLAQWIES QPWDAIDLDN PQDRAQVTQL
LEGLVQELQK KAEHQVGEDG FLLKIKLGHY VHVSSRTRTT AAPWSWLRCI RHILYNEQRL
VREATNGNSS AGILVDAMSQ KHLQINQTFE ELRLVTQDTE NELKKLQQTQ EYFIIQYQES
LRIQAQFAQL AQLNPQERLS RETALQQKQV SLEAWLQREA QTLQQYRVEL AEKHQKTLQL
LRKQQTIILD DELIQWKRRH DWRGMEAPPR SLDVLQSWCE KLAEIIWQNR QQIRRAEHLC
QQLPIPGPVE EMLAEVNATI TDIISALVTS TFIIEKQPPQ VLKTQTKFAA TVRLLVGGKL
NVHMNPPQVK ATIISEQQAK SLLKNENTRN ECSGEILNNC CVMEYHQRTG TLSAHFRNMS
LKRIKRADRR GAESVTEEKF TVLFESQFSV GSNELVFQVK TLSLPVVVIV HGSQDHNATA
TVLWDNAFAE PGRVPFAVPD KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL LFLAQKLFNN
SSSHLEDYNG MSVSWSQFNR ENLPGWNYTF WQWFDGVMEV LKKHHKPHWN DGAILGFVNK
QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSPDRNL WNLKPFTTRE GSIRSLADRL
GDLNYLIYVF PDRPKDEVFS KYYTPVLAKA VDGYVKPQIK QVVPEFVSAS ADSAGSSATY
MDQAPSPAVC PQPHYNMYPQ NPDPVLDQDG EFDLDETMDV ARHVEELLRR PNGQSGPLSP
PPAGLFTPAR GSLS
