ID   STAR3_SALFO             Reviewed;         294 AA.
AC   Q90ZB9;
DT   06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=StAR-related lipid transfer protein 3 {ECO:0000250|UniProtKB:Q14849};
DE   AltName: Full=MLN64-like protein {ECO:0000250|UniProtKB:Q14849};
DE   AltName: Full=START domain-containing protein 3 {ECO:0000250|UniProtKB:Q14849};
DE            Short=StARD3 {ECO:0000250|UniProtKB:Q14849};
DE   Flags: Fragment;
GN   Name=stard3 {ECO:0000250|UniProtKB:Q14849};
GN   Synonyms=mln64 {ECO:0000250|UniProtKB:Q14849};
OS   Salvelinus fontinalis (Brook trout) (Salmo fontinalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC   Salmonidae; Salmoninae; Salvelinus.
OX   NCBI_TaxID=8038;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Goetz F.W.;
RT   "Cloning and characterization of trout MLN64.";
RL   Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Sterol-binding protein that mediates cholesterol transport
CC       from the endoplasmic reticulum to endosomes. Creates contact site
CC       between the endoplasmic reticulum and late endosomes: localizes to late
CC       endosome membranes and contacts the endoplasmic reticulum. Acts as a
CC       lipid transfer protein that redirects sterol to the endosome at the
CC       expense of the cell membrane and favors membrane formation inside
CC       endosomes. {ECO:0000250|UniProtKB:Q14849}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q14849}.
CC   -!- SUBCELLULAR LOCATION: Late endosome membrane
CC       {ECO:0000250|UniProtKB:Q14849}; Multi-pass membrane protein
CC       {ECO:0000255}. Note=Localizes to contact sites between the endoplasmic
CC       reticulum and late endosomes: associates with the endoplasmic reticulum
CC       membrane via interaction with VAPA and VAPB.
CC       {ECO:0000250|UniProtKB:Q14849}.
CC   -!- DOMAIN: The START domain mediates lipid-transfer between membranes. It
CC       contains a hydrophobic cavity able to accommodate one lipid molecule,
CC       thereby serving as a 'hydrophobic bridge' across the aqueous gap
CC       between donor and acceptor organelle membranes.
CC       {ECO:0000250|UniProtKB:Q14849}.
CC   -!- DOMAIN: The MENTAL domain anchors the protein in endosome membranes and
CC       exposes the START domain in the cytosol.
CC       {ECO:0000250|UniProtKB:Q14849}.
CC   -!- SIMILARITY: Belongs to the STARD3 family. {ECO:0000305}.
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DR   EMBL; AF284379; AAK82981.1; -; mRNA.
DR   AlphaFoldDB; Q90ZB9; -.
DR   SMR; Q90ZB9; -.
DR   GO; GO:0140284; C:endoplasmic reticulum-endosome membrane contact site; ISS:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031902; C:late endosome membrane; ISS:UniProtKB.
DR   GO; GO:0044232; C:organelle membrane contact site; ISS:UniProtKB.
DR   GO; GO:0015485; F:cholesterol binding; ISS:UniProtKB.
DR   GO; GO:0120020; F:cholesterol transfer activity; IEA:InterPro.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0030301; P:cholesterol transport; ISS:UniProtKB.
DR   GO; GO:0099044; P:vesicle tethering to endoplasmic reticulum; ISS:UniProtKB.
DR   CDD; cd08906; START_STARD3-like; 1.
DR   Gene3D; 3.30.530.20; -; 1.
DR   InterPro; IPR019498; MENTAL.
DR   InterPro; IPR000799; StAR-like.
DR   InterPro; IPR029867; STARD3_MLN64_C.
DR   InterPro; IPR023393; START-like_dom_sf.
DR   InterPro; IPR002913; START_lipid-bd_dom.
DR   Pfam; PF01852; START; 1.
DR   PRINTS; PR00978; STARPROTEIN.
DR   SMART; SM00234; START; 1.
DR   PROSITE; PS51439; MENTAL; 1.
DR   PROSITE; PS50848; START; 1.
PE   2: Evidence at transcript level;
KW   Endosome; Lipid transport; Lipid-binding; Membrane; Transmembrane;
KW   Transport.
FT   CHAIN           <1..294
FT                   /note="StAR-related lipid transfer protein 3"
FT                   /id="PRO_0000220656"
FT   DOMAIN          <1..66
FT                   /note="MENTAL"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00770"
FT   DOMAIN          79..292
FT                   /note="START"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00197"
FT   REGION          58..77
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           56..61
FT                   /note="FFAT"
FT                   /evidence="ECO:0000250|UniProtKB:Q14849"
FT   NON_TER         1
SQ   SEQUENCE   294 AA;  32807 MW;  E1C3F2CB32C2BA91 CRC64;
     DGYICNNGMD KEERKMCGLL LLPLRSVSPS AYLAAVNAAS ERAPMIYPRA VSEGQFYSPP
     ESLAGSEEDL DEEGLGRRAV SPQEKALVRQ GKEAMAVVEQ ILAQEENWKF EKNNDVGDSV
     YTLEIPFHGK TFILKAFMQC PAELVYQEVI LQPEKMAQWN KTVSGCQILQ RVDDNTLVSY
     DIASGAAGGV VSARDFVNVR RVERKRDCYV SAGMATDHDG KPPHSRYVRG ENGPGGFVVL
     KSSSNPSVCT FIWVLNTDLK GRLPRYLIHQ SLAATMFEFM AHLRQRIRDL RSTR