ID   STA_STRLA               Reviewed;         189 AA.
AC   P08457;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Streptothricin acetyltransferase {ECO:0000303|PubMed:3015852, ECO:0000303|PubMed:3106324};
DE            Short=STAT {ECO:0000303|PubMed:3106324};
DE            EC=2.3.-.- {ECO:0000269|PubMed:3624064};
GN   Name=sta {ECO:0000303|PubMed:3106324};
OS   Streptomyces lavendulae.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1914;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3106324; DOI=10.1128/jb.169.5.1929-1937.1987;
RA   Horinouchi S., Furuya K., Nishiyama M., Suzuki H., Beppu T.;
RT   "Nucleotide sequence of the streptothricin acetyltransferase gene from
RT   Streptomyces lavendulae and its expression in heterologous hosts.";
RL   J. Bacteriol. 169:1929-1937(1987).
RN   [2]
RP   PRELIMINARY FUNCTION.
RX   PubMed=3015852; DOI=10.7164/antibiotics.39.688;
RA   Kobayashi T., Uozumi T., Beppu T.;
RT   "Cloning and characterization of the streptothricin-resistance gene which
RT   encodes streptothricin acetyltransferase from Streptomyces lavendulae.";
RL   J. Antibiot. 39:688-693(1986).
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=3624064; DOI=10.7164/antibiotics.40.1016;
RA   Kobayashi T., Horinouchi S., Uozumi T., Beppu T.;
RT   "Purification and biochemical characterization of streptothricin
RT   acetyltransferase coded by the cloned streptothricin-resistance gene of
RT   Streptomyces lavendulae.";
RL   J. Antibiot. 40:1016-1022(1987).
CC   -!- FUNCTION: Involved in resistance to streptothricin, a broad-spectrum
CC       antibiotic produced by streptomycetes. Detoxifies streptothricin via
CC       acetylation of the beta amino group of the first beta-lysyl moiety of
CC       streptothricin. {ECO:0000269|PubMed:3624064,
CC       ECO:0000305|PubMed:3015852}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + streptothricin F = CoA + H(+) + N(beta)-
CC         acetylstreptothricin F; Xref=Rhea:RHEA:57000, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:60822,
CC         ChEBI:CHEBI:141394; Evidence={ECO:0000269|PubMed:3624064};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=69 uM for S-acetyl CoA {ECO:0000269|PubMed:3624064};
CC         KM=2.3 uM for streptothricin F {ECO:0000269|PubMed:3624064};
CC       pH dependence:
CC         Optimum pH is 7.8. {ECO:0000269|PubMed:3624064};
CC       Temperature dependence:
CC         Stable at 35 degrees Celsius. Rapid inactivation occurs at 50 degrees
CC         Celsius. {ECO:0000269|PubMed:3624064};
CC   -!- SIMILARITY: Belongs to the acetyltransferase family. GNAT subfamily.
CC       {ECO:0000305}.
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DR   EMBL; M16183; AAA88560.1; -; Genomic_DNA.
DR   PIR; A26872; A26872.
DR   RefSeq; WP_063854936.1; NG_048077.1.
DR   AlphaFoldDB; P08457; -.
DR   SMR; P08457; -.
DR   BioCyc; MetaCyc:MON-15932; -.
DR   GO; GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR000182; GNAT_dom.
DR   Pfam; PF00583; Acetyltransf_1; 1.
DR   SUPFAM; SSF55729; SSF55729; 1.
DR   PROSITE; PS51186; GNAT; 1.
PE   1: Evidence at protein level;
KW   Acyltransferase; Antibiotic resistance; Transferase.
FT   CHAIN           1..189
FT                   /note="Streptothricin acetyltransferase"
FT                   /id="PRO_0000068587"
FT   DOMAIN          44..189
FT                   /note="N-acetyltransferase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00532"
FT   REGION          55..76
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   189 AA;  20032 MW;  7BCA8AA2682654AC CRC64;
     MTTTHGSTYE FRSARPGDAE AIEGLDGSFT TSTVFEVDVT GDGFALREVP ADPPLVKVFP
     DDGGSDGEDG AEGEDADSRT FVAVGADGDL AGFAAVSYSA WNQRLTIEDI EVAPGHRGKG
     IGRVLMRHAA DFARERGAGH LWLEVTNVNA PAIHAYRRMG FAFCGLDSAL YQGTASEGEH
     ALYMSMPCP