STBB_PSESM
ID STBB_PSESM Reviewed; 139 AA.
AC Q52562;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 11-APR-2003, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Plasmid stability protein StbB;
DE AltName: Full=Ribonuclease StbB;
DE Short=RNase StbB;
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE AltName: Full=Toxin StbB;
GN Name=stbB; OrderedLocusNames=PSPTO_B0018;
OS Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OG Plasmid pDC3000B.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=223283;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9326365; DOI=10.1016/s0014-5793(97)01089-2;
RA Hanekamp T., Kobayashi D., Hayes S., Stayton M.M.;
RT "Avirulence gene D of Pseudomonas syringae pv. tomato may have undergone
RT horizontal gene transfer.";
RL FEBS Lett. 415:40-44(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000; PLASMID=pDC3000B;
RX PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC RNase (By similarity). Involved in plasmid stability.
CC {ECO:0000255|HAMAP-Rule:MF_00265}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC {ECO:0000255|HAMAP-Rule:MF_00265}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L48985; AAB81645.1; -; Genomic_DNA.
DR EMBL; AE016854; AAO59109.1; -; Genomic_DNA.
DR RefSeq; NP_808606.1; NC_004632.1.
DR RefSeq; WP_005769733.1; NC_004632.1.
DR AlphaFoldDB; Q52562; -.
DR SMR; Q52562; -.
DR EnsemblBacteria; AAO59109; AAO59109; PSPTO_B0018.
DR GeneID; 1187396; -.
DR KEGG; pst:PSPTO_B0018; -.
DR PATRIC; fig|223283.9.peg.5772; -.
DR HOGENOM; CLU_118482_8_2_6; -.
DR OMA; VINPWEA; -.
DR OrthoDB; 1968541at2; -.
DR PhylomeDB; Q52562; -.
DR Proteomes; UP000002515; Plasmid pDC3000B.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR HAMAP; MF_00265; VapC_Nob1; 1.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR002716; PIN_dom.
DR InterPro; IPR022907; VapC_family.
DR Pfam; PF01850; PIN; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
PE 3: Inferred from homology;
KW Hydrolase; Magnesium; Metal-binding; Nuclease; Plasmid; Reference proteome;
KW Toxin-antitoxin system.
FT CHAIN 1..139
FT /note="Plasmid stability protein StbB"
FT /id="PRO_0000072256"
FT DOMAIN 2..136
FT /note="PINc"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT BINDING 5
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT BINDING 104
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT CONFLICT 35
FT /note="L -> M (in Ref. 1; AAB81645)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 139 AA; 15138 MW; 0E1295211B127D72 CRC64;
MILLDTNVIS EPQRREPNAH VLDWIDAQAL ETLYLSTITV AELRAGIALM PVGKRQDSLR
ENLEKHLLPM FANRVLSFDM TCTKAYAELL AKSRAAGLAV ETADAFIAAI ALANGFTVAT
RDTGPFEAAG LNVINPWEA
