STCD_RHIME
ID STCD_RHIME Reviewed; 678 AA.
AC O87278;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2001, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Probable N-methylproline demethylase;
DE EC=1.-.-.-;
DE AltName: Full=Stachydrine utilization protein StcD;
GN Name=stcD; Synonyms=stcD2; OrderedLocusNames=RB1010; ORFNames=SMb21570;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OG Plasmid pSymB (megaplasmid 2).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=1021;
RX PubMed=9758825; DOI=10.1128/aem.64.10.3954-3960.1998;
RA Phillips D.A., Sande E.S., Vriezen J.A.C., de Bruijn F.J., Le Rudulier D.,
RA Joseph C.M.;
RT "A new genetic locus in Sinorhizobium meliloti is involved in stachydrine
RT utilization.";
RL Appl. Environ. Microbiol. 64:3954-3960(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481431; DOI=10.1073/pnas.161294698;
RA Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT endosymbiont Sinorhizobium meliloti.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- FUNCTION: Possible NADH-dependent oxidase, functions as a demethylase
CC that converts N-methylproline to proline. {ECO:0000269|PubMed:9758825}.
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000250|UniProtKB:P42593};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000250|UniProtKB:P42593};
CC -!- PATHWAY: Amine and polyamine degradation; stachydrine degradation.
CC -!- INDUCTION: By stachydrine.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC63907.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF016307; AAC63907.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AL591985; CAC49410.1; -; Genomic_DNA.
DR PIR; B95968; B95968.
DR RefSeq; NP_437550.1; NC_003078.1.
DR RefSeq; WP_010975854.1; NC_003078.1.
DR AlphaFoldDB; O87278; -.
DR SMR; O87278; -.
DR STRING; 266834.SM_b21570; -.
DR EnsemblBacteria; CAC49410; CAC49410; SM_b21570.
DR GeneID; 61600970; -.
DR KEGG; sme:SM_b21570; -.
DR PATRIC; fig|266834.11.peg.5935; -.
DR eggNOG; COG0446; Bacteria.
DR eggNOG; COG1902; Bacteria.
DR HOGENOM; CLU_012153_1_2_5; -.
DR OMA; NQACIAY; -.
DR BioCyc; MetaCyc:MON-2221; -.
DR UniPathway; UPA00255; -.
DR Proteomes; UP000001976; Plasmid pSymB.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0019504; P:stachydrine catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.20.20.70; -; 1.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR001155; OxRdtase_FMN_N.
DR Pfam; PF00724; Oxidored_FMN; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 2: Evidence at transcript level;
KW FAD; Flavoprotein; FMN; NAD; Oxidoreductase; Plasmid; Reference proteome.
FT CHAIN 1..678
FT /note="Probable N-methylproline demethylase"
FT /id="PRO_0000194491"
FT BINDING 59
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250|UniProtKB:P42593"
FT BINDING 102
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250|UniProtKB:P42593"
FT BINDING 220
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250|UniProtKB:P42593"
FT BINDING 299
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250|UniProtKB:P42593"
FT BINDING 321..322
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250|UniProtKB:P42593"
FT BINDING 396
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:P42593"
FT BINDING 415
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:P42593"
FT BINDING 423
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:P42593"
FT BINDING 433
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:P42593"
FT BINDING 460
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250|UniProtKB:P42593"
FT CONFLICT 6..7
FT /note="LL -> FF (in Ref. 1; AAC63907)"
FT /evidence="ECO:0000305"
FT CONFLICT 63..91
FT /note="VSKDSPPVFNNLLAYRDEIVPWIREMTDA -> SRRTARRSSTICSPTGTRS
FT CRGSGRCRP (in Ref. 1; AAC63907)"
FT /evidence="ECO:0000305"
FT CONFLICT 95..97
FT /note="EGA -> RGR (in Ref. 1; AAC63907)"
FT /evidence="ECO:0000305"
FT CONFLICT 591
FT /note="Y -> I (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 678 AA; 74684 MW; 329E22EDD928ED1B CRC64;
MPNDPLLQPY QLKHLTLRNR IIVTAHEPAY PEDGMPKERY RAYTVERARG GVAMTMTAGS
AAVSKDSPPV FNNLLAYRDE IVPWIREMTD AVHEEGAVIM IQLTHLGRRT RWDKGDWLPV
VAPSHHREAA HRAFPKKIED WDIDRIIKDF ADAAERMKAG GMDGVELEAY GHLIDQFASP
LTNELDGPYG GSLDNRMRFC FDVLKAIRAR VGDEFILGVR YTADECLPGG TDKAEGLEIS
KRLKESGLID YLNIIRGHID TDPGLTDVIP IQGMANSPHL DFAGEIRAAT NFPTFHAAKI
PDVATARHAI ASGKVDMVGM TRAHMTDPHI VRKIIEKREE DIRPCVGANY CLDRIYQGGA
AYCIHNAATG RELTMPHSIA KAHCRRKVVV VGTGPAGLEA ARVAGERGHE VIVFEAASDP
GGQVRLTAQS PRRREMISII DWRMSQCEKL GVTFHFNTWA EAEAIQAESP DVVIIATGGL
PHTEVLSRGN ELVVSAWDII SGDAKPGTNV LIFDDAGDHA ALQAAEFLAT AGARVEIMTP
DRSFAPEVMA MNLVPYMRCL QKLDVTFTVT YRLEAVEKSG NELVAHVGSD YGGISKQRTF
DQVVVNHGTI PLDELYFELK PFSSNLGEIA HDQMIAGEPQ SVVRNAEGKF QLFRIGDAVA
ARNTHAAIYD ALRLLKDI
