ID   STCN2_ECHES             Reviewed;          89 AA.
AC   A0A144LVM3;
DT   15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT   08-JUN-2016, sequence version 1.
DT   25-MAY-2022, entry version 8.
DE   RecName: Full=Strongylocin 2 {ECO:0000312|EMBL:AMT92376.1};
DE   AltName: Full=EeStrongylocin 2 {ECO:0000303|PubMed:27007817};
DE   Flags: Precursor;
OS   Echinus esculentus (Sea urchin).
OC   Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC   Euechinoidea; Echinacea; Camarodonta; Echinidea; Echinidae; Echinus.
OX   NCBI_TaxID=7648 {ECO:0000312|EMBL:AMT92376.1};
RN   [1] {ECO:0000312|EMBL:AMT92376.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 39-48; 50-55 AND 81-89,
RP   FUNCTION, PRESENCE OF DISULFIDE BONDS, MASS SPECTROMETRY, AND BROMINATION
RP   AT TRP-39.
RC   TISSUE=Coelomocyte {ECO:0000303|PubMed:27007817};
RX   PubMed=27007817; DOI=10.1371/journal.pone.0151820;
RA   Solstad R.G., Li C., Isaksson J., Johansen J., Svenson J., Stensvag K.,
RA   Haug T.;
RT   "Novel Antimicrobial Peptides EeCentrocins 1, 2 and EeStrongylocin 2 from
RT   the Edible Sea Urchin Echinus esculentus Have 6-Br-Trp Post-Translational
RT   Modifications.";
RL   PLoS ONE 11:E0151820-E0151820(2016).
CC   -!- FUNCTION: Has antimicrobial activity against Gram-negative bacteria and
CC       Gram-positive bacteria with minimum inhibitory concentration (MIC)
CC       between 0.78 uM and 3.13 uM. {ECO:0000269|PubMed:27007817}.
CC   -!- PTM: Contains 3 disulfide bonds. {ECO:0000269|PubMed:27007817}.
CC   -!- MASS SPECTROMETRY: Mass=5917.77; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:27007817};
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DR   EMBL; KR494264; AMT92376.1; -; mRNA.
DR   AlphaFoldDB; A0A144LVM3; -.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bromination; Direct protein sequencing;
KW   Disulfide bond; Fungicide; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..38
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000438846"
FT   PEPTIDE         39..89
FT                   /note="Strongylocin 2"
FT                   /evidence="ECO:0000305|PubMed:27007817"
FT                   /id="PRO_0000438847"
FT   MOD_RES         39
FT                   /note="6'-bromotryptophan"
FT                   /evidence="ECO:0000269|PubMed:27007817"
SQ   SEQUENCE   89 AA;  10298 MW;  9525D0595ED4A8CF CRC64;
     MNIRTASFTF IVVMMILSQT MADRFFNEPE EDDHLVESWN PFKKIAHRHC YPKNECITTN
     GKKTCKDYSC CQIVLFGKKT RSACTVVAQ