STE3_YEAST
ID STE3_YEAST Reviewed; 470 AA.
AC P06783; D6VX23;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Pheromone a factor receptor;
GN Name=STE3; OrderedLocusNames=YKL178C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3006051; DOI=10.1073/pnas.83.5.1418;
RA Hagen D.C., McCaffrey G., Sprague G.F. Jr.;
RT "Evidence the yeast STE3 gene encodes a receptor for the peptide pheromone
RT a factor: gene sequence and implications for the structure of the presumed
RT receptor.";
RL Proc. Natl. Acad. Sci. U.S.A. 83:1418-1422(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=16453635; DOI=10.1002/j.1460-2075.1985.tb03982.x;
RA Nakayama N., Miyajima A., Arai K.;
RT "Nucleotide sequences of STE2 and STE3, cell type-specific sterile genes
RT from Saccharomyces cerevisiae.";
RL EMBO J. 4:2643-2648(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8154185; DOI=10.1002/yea.320091208;
RA Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J.,
RA Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W.;
RT "Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI
RT from Saccharomyces cerevisiae reveals 23 open reading frames including the
RT FAS1 gene.";
RL Yeast 9:1343-1348(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Receptor for the peptide pheromone a factor.
CC -!- INTERACTION:
CC P06783; D6VTK4: STE2; NbExp=2; IntAct=EBI-18366, EBI-6323511;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 4 family.
CC {ECO:0000305}.
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DR EMBL; M12239; AAA35113.1; -; Genomic_DNA.
DR EMBL; X03011; CAA26795.1; -; Genomic_DNA.
DR EMBL; X74151; CAA52261.1; -; Genomic_DNA.
DR EMBL; Z28177; CAA82019.1; -; Genomic_DNA.
DR EMBL; AY692742; AAT92761.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA08989.1; -; Genomic_DNA.
DR PIR; B24670; B24670.
DR RefSeq; NP_012743.1; NM_001179744.1.
DR AlphaFoldDB; P06783; -.
DR BioGRID; 33961; 21.
DR DIP; DIP-1146N; -.
DR IntAct; P06783; 3.
DR MINT; P06783; -.
DR STRING; 4932.YKL178C; -.
DR TCDB; 9.B.45.2.1; the fungal mating-type pheromone receptor (mat-pr) family.
DR iPTMnet; P06783; -.
DR PaxDb; P06783; -.
DR PRIDE; P06783; -.
DR DNASU; 853677; -.
DR EnsemblFungi; YKL178C_mRNA; YKL178C; YKL178C.
DR GeneID; 853677; -.
DR KEGG; sce:YKL178C; -.
DR SGD; S000001661; STE3.
DR VEuPathDB; FungiDB:YKL178C; -.
DR eggNOG; ENOG502S44N; Eukaryota.
DR HOGENOM; CLU_027592_4_0_1; -.
DR InParanoid; P06783; -.
DR OMA; IPPLIWH; -.
DR BioCyc; YEAST:G3O-31944-MON; -.
DR PRO; PR:P06783; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P06783; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:SGD.
DR GO; GO:0005775; C:vacuolar lumen; IDA:CAFA.
DR GO; GO:0004933; F:mating-type a-factor pheromone receptor activity; IMP:SGD.
DR GO; GO:0004932; F:mating-type factor pheromone receptor activity; IBA:GO_Central.
DR GO; GO:0000755; P:cytogamy; IDA:SGD.
DR GO; GO:0000750; P:pheromone-dependent signal transduction involved in conjugation with cellular fusion; IMP:SGD.
DR InterPro; IPR001499; GPCR_STE3.
DR PANTHER; PTHR28097; PTHR28097; 1.
DR Pfam; PF02076; STE3; 1.
DR PRINTS; PR00899; GPCRSTE3.
PE 1: Evidence at protein level;
KW G-protein coupled receptor; Membrane; Pheromone response; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..470
FT /note="Pheromone a factor receptor"
FT /id="PRO_0000195079"
FT TOPO_DOM 1..5
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 6..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 24..29
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..70
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 99..116
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 135..155
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..205
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 229..266
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..470
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 300..470
FT /note="Hydrophilic"
FT REGION 440..470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 470 AA; 53708 MW; 5DBF611076B8CA91 CRC64;
MSYKSAIIGL CLLAVILLAP PLAWHSHTKN IPAIILITWL LTMNLTCIVD AAIWSDDDFL
TRWDGKGWCD IVIKLQVGAN IGISCAVTNI IYNLHTILKA DSVLPDLSSW TKIVKDLVIS
LFTPVMVMGF SYLLQVFRYG IARYNGCQNL LSPTWITTVL YTMWMLIWSF VGAVYATLVL
FVFYKKRKDV RDILHCTNSG LNLTRFARLL IFCFIIILVM FPFSVYTFVQ DLQQVEGHYT
FKNTHSSTIW NTIIKFDPGR PIYNIWLYVL MSYLVFLIFG LGSDALHMYS KFLRSIKLGF
VLDMWKRFID KNKEKRVGIL LNKLSSRKES RNPFSTDSEN YISTCTENYS PCVGTPISQA
HFYVDYRIPD DPRKSQNKSK KYLFADKETD DILDEIDLKE SRHIPYVTQG QSFDDEISLG
GFSKVTLDYS EKLHNSASSN FEGESLCYSP ASKEENSSSN EHSSENTAGP
