ID   STE6_SCHPO              Reviewed;         911 AA.
AC   P26674; Q9UUM8;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-MAY-2022, entry version 149.
DE   RecName: Full=Protein ste6;
GN   Name=ste6; ORFNames=SPCC1442.01, SPCC1450.17;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2107403; DOI=10.1038/344355a0;
RA   Hughes D.A., Fukui Y., Yamamoto M.;
RT   "Homologous activators of ras in fission and budding yeast.";
RL   Nature 344:355-357(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Promotes the exchange of RAS-bound GDP by GTP. This protein
CC       is essential for mating.
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DR   EMBL; X53254; CAA37345.1; -; Genomic_DNA.
DR   EMBL; CU329672; CAB40184.1; -; Genomic_DNA.
DR   PIR; S28098; S28098.
DR   RefSeq; NP_588316.1; NM_001023306.2.
DR   AlphaFoldDB; P26674; -.
DR   SMR; P26674; -.
DR   BioGRID; 275308; 16.
DR   STRING; 4896.SPCC1442.01.1; -.
DR   PaxDb; P26674; -.
DR   EnsemblFungi; SPCC1442.01.1; SPCC1442.01.1:pep; SPCC1442.01.
DR   GeneID; 2538724; -.
DR   KEGG; spo:SPCC1442.01; -.
DR   PomBase; SPCC1442.01; ste6.
DR   VEuPathDB; FungiDB:SPCC1442.01; -.
DR   eggNOG; KOG3417; Eukaryota.
DR   HOGENOM; CLU_321091_0_0_1; -.
DR   InParanoid; P26674; -.
DR   OMA; GWCDGIC; -.
DR   Reactome; R-SPO-416482; G alpha (12/13) signalling events.
DR   Reactome; R-SPO-8980692; RHOA GTPase cycle.
DR   Reactome; R-SPO-9013148; CDC42 GTPase cycle.
DR   PRO; PR:P26674; -.
DR   Proteomes; UP000002485; Chromosome III.
DR   GO; GO:1990819; C:actin fusion focus; IDA:PomBase.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; EXP:PomBase.
DR   GO; GO:0000747; P:conjugation with cellular fusion; IMP:PomBase.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR   GO; GO:0062038; P:positive regulation of pheromone response MAPK cascade; IMP:PomBase.
DR   GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR   CDD; cd00155; RasGEF; 1.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   InterPro; IPR036028; SH3-like_dom_sf.
DR   InterPro; IPR001452; SH3_domain.
DR   PANTHER; PTHR23113; PTHR23113; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   Pfam; PF00618; RasGEF_N; 1.
DR   Pfam; PF00018; SH3_1; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SMART; SM00229; RasGEFN; 1.
DR   SMART; SM00326; SH3; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   SUPFAM; SSF50044; SSF50044; 1.
DR   PROSITE; PS00720; RASGEF; 1.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
DR   PROSITE; PS50002; SH3; 1.
PE   4: Predicted;
KW   Guanine-nucleotide releasing factor; Reference proteome; SH3 domain.
FT   CHAIN           1..911
FT                   /note="Protein ste6"
FT                   /id="PRO_0000068899"
FT   DOMAIN          1..60
FT                   /note="SH3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT   DOMAIN          489..623
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT   DOMAIN          663..894
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
SQ   SEQUENCE   911 AA;  105186 MW;  5F8C12D20C4B753F CRC64;
     MRFQTTAISD YENSSNPSFL KFSAGDTIIV IEVLEDGWCD GICSEKRGWF PTSCIDSSKI
     QNFFSSFHSS NEKDPNAQCC APFHVEAHLQ DSAWFEKHGV QAINSIPSSE EFLRKNLQND
     IHHLVKGILT TAAAVSQSIK KEGTQVIVFG IETVRSMVLS FPLIILSTLD ENFLSEVAQV
     FSSLNLLPEL SRMGCTYGEL CIRFTKLLKQ LANKFLFFFR PDVSFPSYFL GSLIAHEIHF
     LPWDFNMLCS NSVQSAHTNL QPDITSFVAI LSLSHEAYHC TENEFWNLEA QKLTENTTQK
     VLQLVAEDAL EAWKLDILED IDRCIQCCRR FLSANQRINY SSSENNPFSF TSQDVEALKD
     ELSSNLCDLY LWSIDLEQIS PSDCLLDNYS LFVDLLVTLK VSLLRIKSII VQFSERIVFL
     SLEYKFLTNI QPELNDAEKS QLDGFDLNKT NWFDSKGLVC YLMKQTSPEP LLIRNLLFSF
     WSCNGKIEQD GKIKTATLVF IINYLLRTDI DSTFFTTIFL NTYASMISSS DLFSILGAHF
     RFICSLNFGK ISFISHEFYR VSKRFLDILL IWFESYLVEE LDNSKSIFFL FKIYKVFEVF
     VVPHFASAEE LLHSLSHLLH HPSTKRSHKM LEGKELSQEL EDLSLHNSPD PIIYKDELVL
     LLPPREIAKQ LCILEFQSFS HISRIQFLTK IWDNLNRFSP KEKTSTFYLS NHLVNFVTET
     IVQEEEPRRR TNVLAYFIQV CDYLRELNNF ASLFSIISAL NSSPIHRLRK TWANLNSKTL
     ASFELLNNLT EARKNFSNYR DCLENCVLPC VPFLGVYFTD LTFLKTGNKD NFQNMINFDK
     RTKVTRILNE IKKFQSVGYM FNPINEVQEL LNEVISRERN TNNIYQRSLT VEPRESEDQA
     LQRLLIDSGI F