STEEP_DROME
ID STEEP_DROME Reviewed; 247 AA.
AC Q9V412;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=STING ER exit protein {ECO:0000250|UniProtKB:Q9H5V9};
DE Short=STEEP {ECO:0000250|UniProtKB:Q9H5V9};
GN Name=Steep1 {ECO:0000250|UniProtKB:Q9H5V9}; ORFNames=CG16865;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-127, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Stimulates membrane curvature formation and subsequent
CC endoplasmic reticulum exit site (ERES) establishment by recruiting PI3K
CC complex I, leading to COPII vesicle-mediated transport (By similarity).
CC Promotes endoplasmic reticulum (ER) exit of cGAMP-activated STING1
CC oligomers (By similarity). {ECO:0000250|UniProtKB:Q9H5V9}.
CC -!- SIMILARITY: Belongs to the STEEP1 family. {ECO:0000305}.
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DR EMBL; AE014134; AAF53334.1; -; Genomic_DNA.
DR EMBL; AY113531; AAM29536.1; -; mRNA.
DR RefSeq; NP_001260451.1; NM_001273522.1.
DR RefSeq; NP_609677.1; NM_135833.4.
DR AlphaFoldDB; Q9V412; -.
DR BioGRID; 60821; 3.
DR IntAct; Q9V412; 1.
DR STRING; 7227.FBpp0304016; -.
DR iPTMnet; Q9V412; -.
DR PaxDb; Q9V412; -.
DR DNASU; 34788; -.
DR EnsemblMetazoa; FBtr0080540; FBpp0080117; FBgn0028919.
DR EnsemblMetazoa; FBtr0331626; FBpp0304016; FBgn0028919.
DR GeneID; 34788; -.
DR KEGG; dme:Dmel_CG16865; -.
DR UCSC; CG16865-RA; d. melanogaster.
DR FlyBase; FBgn0028919; CG16865.
DR VEuPathDB; VectorBase:FBgn0028919; -.
DR eggNOG; KOG4397; Eukaryota.
DR GeneTree; ENSGT00390000002197; -.
DR HOGENOM; CLU_099571_1_0_1; -.
DR InParanoid; Q9V412; -.
DR OMA; GIEKQHR; -.
DR OrthoDB; 1161089at2759; -.
DR PhylomeDB; Q9V412; -.
DR SignaLink; Q9V412; -.
DR BioGRID-ORCS; 34788; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34788; -.
DR PRO; PR:Q9V412; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0028919; Expressed in egg cell and 20 other tissues.
DR ExpressionAtlas; Q9V412; baseline and differential.
DR Genevisible; Q9V412; DM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0090158; P:endoplasmic reticulum membrane organization; ISS:UniProtKB.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IC:FlyBase.
DR InterPro; IPR029704; STEEP-like.
DR PANTHER; PTHR46355; PTHR46355; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Phosphoprotein; Reference proteome.
FT CHAIN 1..247
FT /note="STING ER exit protein"
FT /id="PRO_0000287615"
FT REGION 215..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 195..216
FT /evidence="ECO:0000255"
FT MOD_RES 127
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 247 AA; 27696 MW; 2C80432C61389D78 CRC64;
MPKVVSRSIV CSDTKDQEEY NEEKPLNIYY CLCNKMALIL DCTLEQLPLR EVDNARVINA
NDHANKLTHN PTPRMVYIKR KSRGNGIEKQ YRYKCRSCSL PLYYRHSPDS HVTFVMSNAL
IRNKGESPLT QLLNSEIKGS FKAPAAKPAT SAGPDDSGIV DASGKKVVVT RHTKNMGKFS
SVTVSTIDEE EDEIEAREIA DSYANNARII EKQLQRKGGK LSDVGIKTKT EDAPPPQKKQ
RGTLLER
