STEP2_ARATH
ID STEP2_ARATH Reviewed; 202 AA.
AC Q9SJ02; Q94F34;
DT 29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Stress enhanced protein 2, chloroplastic {ECO:0000305};
DE Flags: Precursor;
GN Name=SEP2 {ECO:0000303|PubMed:10725357}; OrderedLocusNames=At2g21970;
GN ORFNames=F7D8.29;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION BY LIGHT, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=10725357; DOI=10.1073/pnas.97.7.3741;
RA Heddad M., Adamska I.;
RT "Light stress-regulated two-helix proteins in Arabidopsis thaliana related
RT to the chlorophyll a/b-binding gene family.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:3741-3746(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May be involved in non-photochemical quenching, a process
CC that maintains the balance between dissipation and utilization of light
CC energy to minimize generation of oxidizing molecules, thereby
CC protecting the plant against photo-oxidative damage (By similarity).
CC May play a photoprotective role in the thylakoid membrane in response
CC to light stress (Probable). {ECO:0000250|UniProtKB:Q9XF91,
CC ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:10725357}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SJ02-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SJ02-2; Sequence=VSP_046518;
CC -!- INDUCTION: Present at low levels under low light conditions, but
CC accumulates under high-intensity light. Induced by UV-A illumination.
CC Fades out upon wounding. {ECO:0000269|PubMed:10725357}.
CC -!- SIMILARITY: Belongs to the ELIP/psbS family. {ECO:0000305}.
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DR EMBL; AF133717; AAF61626.1; -; mRNA.
DR EMBL; AC007019; AAD20414.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07247.1; -; Genomic_DNA.
DR EMBL; AF386927; AAK62372.1; -; mRNA.
DR EMBL; BT006554; AAP21362.1; -; mRNA.
DR EMBL; AY084392; AAM60969.1; -; mRNA.
DR PIR; D84607; D84607.
DR RefSeq; NP_565524.1; NM_127766.3. [Q9SJ02-1]
DR AlphaFoldDB; Q9SJ02; -.
DR STRING; 3702.AT2G21970.1; -.
DR PaxDb; Q9SJ02; -.
DR PRIDE; Q9SJ02; -.
DR ProteomicsDB; 245216; -. [Q9SJ02-1]
DR EnsemblPlants; AT2G21970.1; AT2G21970.1; AT2G21970. [Q9SJ02-1]
DR GeneID; 816733; -.
DR Gramene; AT2G21970.1; AT2G21970.1; AT2G21970. [Q9SJ02-1]
DR KEGG; ath:AT2G21970; -.
DR Araport; AT2G21970; -.
DR TAIR; locus:2052489; AT2G21970.
DR eggNOG; ENOG502RXQW; Eukaryota.
DR HOGENOM; CLU_1373980_0_0_1; -.
DR OMA; CNTFIDS; -.
DR PRO; PR:Q9SJ02; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SJ02; baseline and differential.
DR Genevisible; Q9SJ02; AT.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009523; C:photosystem II; IEA:UniProtKB-KW.
DR GO; GO:0016168; F:chlorophyll binding; ISS:TAIR.
DR GO; GO:0071486; P:cellular response to high light intensity; IEP:UniProtKB.
DR GO; GO:0071492; P:cellular response to UV-A; IEP:UniProtKB.
DR GO; GO:0009765; P:photosynthesis, light harvesting; ISS:TAIR.
DR GO; GO:0009611; P:response to wounding; IEP:UniProtKB.
DR InterPro; IPR044971; SEP2.
DR PANTHER; PTHR36490; PTHR36490; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Membrane; Photosynthesis;
KW Photosystem II; Plastid; Reference proteome; Thylakoid; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1..60
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 61..202
FT /note="Stress enhanced protein 2, chloroplastic"
FT /id="PRO_0000422368"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..35
FT /note="MAMATRAIRYQLPSPRFRAPRCESSEPIKQIQIQQ -> MQG (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_046518"
SQ SEQUENCE 202 AA; 21989 MW; D300A5B1EE70F6F8 CRC64;
MAMATRAIRY QLPSPRFRAP RCESSEPIKQ IQIQQRPRGG DLAENGKIVL QPRLCTLRSY
GSDMVIAKKD GGDGGGGGSD VELASPFFET LTDYIESSKK SQDFETISGR LAMIVFAVTV
TEEIVTGNSL FKKLDVEGLS EAIGAGLAAM GCAAMFAWLT ISRNRVGRIF TVSCNSFIDS
LVDQIVDGLF YDTKPSDWSD DL
