ID   STF3_MYCTU              Reviewed;         388 AA.
AC   P9WLG1; L0TAP1; P64963; Q50695;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 37.
DE   RecName: Full=PAPS-dependent sulfotransferase Stf3 {ECO:0000303|PubMed:16537518};
DE            EC=2.8.2.- {ECO:0000305};
GN   Name=stf3 {ECO:0000303|PubMed:12144918};
GN   OrderedLocusNames=Rv2267c {ECO:0000312|EMBL:CCP45048.1};
GN   ORFNames=MTCY339.43;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=12144918; DOI=10.1016/s1074-5521(02)00175-8;
RA   Mougous J.D., Green R.E., Williams S.J., Brenner S.E., Bertozzi C.R.;
RT   "Sulfotransferases and sulfatases in mycobacteria.";
RL   Chem. Biol. 9:767-776(2002).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=H37Rv;
RX   PubMed=16537518; DOI=10.1073/pnas.0510861103;
RA   Mougous J.D., Senaratne R.H., Petzold C.J., Jain M., Lee D.H.,
RA   Schelle M.W., Leavell M.D., Cox J.S., Leary J.A., Riley L.W.,
RA   Bertozzi C.R.;
RT   "A sulfated metabolite produced by stf3 negatively regulates the virulence
RT   of Mycobacterium tuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:4258-4263(2006).
RN   [4]
RP   FUNCTION.
RX   PubMed=18928249; DOI=10.1021/cb800145r;
RA   Holsclaw C.M., Sogi K.M., Gilmore S.A., Schelle M.W., Leavell M.D.,
RA   Bertozzi C.R., Leary J.A.;
RT   "Structural characterization of a novel sulfated menaquinone produced by
RT   stf3 from Mycobacterium tuberculosis.";
RL   ACS Chem. Biol. 3:619-624(2008).
CC   -!- FUNCTION: Required for the sulfation of S881. S881 is a sulfated
CC       menaquinone, which is localized in the outer envelope of M.tuberculosis
CC       and negatively regulates its virulence. 3'-phosphoadenosine-5'-
CC       phosphosulfate (PAPS) is the sulfate donor.
CC       {ECO:0000269|PubMed:16537518, ECO:0000269|PubMed:18928249}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant does not synthesize S881 and
CC       shows a hypervirulent phenotype in the mouse model of infection.
CC       {ECO:0000269|PubMed:16537518}.
CC   -!- SIMILARITY: Belongs to the Stf3 family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP45048.1; -; Genomic_DNA.
DR   PIR; G70729; G70729.
DR   RefSeq; NP_216783.1; NC_000962.3.
DR   RefSeq; WP_003411661.1; NZ_NVQJ01000008.1.
DR   AlphaFoldDB; P9WLG1; -.
DR   SMR; P9WLG1; -.
DR   STRING; 83332.Rv2267c; -.
DR   PaxDb; P9WLG1; -.
DR   DNASU; 888487; -.
DR   GeneID; 45426249; -.
DR   GeneID; 888487; -.
DR   KEGG; mtu:Rv2267c; -.
DR   TubercuList; Rv2267c; -.
DR   eggNOG; COG0446; Bacteria.
DR   OMA; FILGHWR; -.
DR   PhylomeDB; P9WLG1; -.
DR   BioCyc; MetaCyc:G185E-6484-MON; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0001517; F:N-acetylglucosamine 6-O-sulfotransferase activity; IBA:GO_Central.
DR   GO; GO:0008146; F:sulfotransferase activity; IMP:MTBBASE.
DR   GO; GO:0006044; P:N-acetylglucosamine metabolic process; IBA:GO_Central.
DR   GO; GO:0046506; P:sulfolipid biosynthetic process; IMP:MTBBASE.
DR   GO; GO:0006790; P:sulfur compound metabolic process; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Transferase.
FT   CHAIN           1..388
FT                   /note="PAPS-dependent sulfotransferase Stf3"
FT                   /id="PRO_0000103993"
SQ   SEQUENCE   388 AA;  46062 MW;  5DED0263275A9B24 CRC64;
     MKALRSSSRL SRWREWAAPL WVGCNFSAWM RLLIRNRFAV HHSRWHFAVL YTFLSMVNSC
     LGLWQKIVFG RRVAETVIAD PPIFIVGHWR TGTTLLHELL VVDDRHTGPT GYECLAPHHF
     LLTEWFAPYV EFLVSKHRAM DNMDLSLHHP QEDEFVWCMQ GLPSPYLTIA FPNRPPQYEE
     YLDLEQVAPR ELEIWKRTLF RFVQQVYFRR RKTVILKNPT HSFRIKVLLE VFPQAKFIHI
     VRDPYVVYPS TIHLHKALYR IHGLQQPTFD GLDDKVVSTY VDLYRKLDEG RELVDPTRFY
     ELRYEDLIGD PEGQLRRLYQ HLGLGDFECY LPRLRQYLAD HADYKTNSYQ LTVEQRAIVD
     EHWGEIIDRY GYDRHTPEPA RLRPAVGG