STHA_ALIF1
ID STHA_ALIF1 Reviewed; 471 AA.
AC Q5E212;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2006, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Soluble pyridine nucleotide transhydrogenase {ECO:0000255|HAMAP-Rule:MF_00247};
DE Short=STH {ECO:0000255|HAMAP-Rule:MF_00247};
DE EC=1.6.1.1 {ECO:0000255|HAMAP-Rule:MF_00247};
DE AltName: Full=NAD(P)(+) transhydrogenase [B-specific] {ECO:0000255|HAMAP-Rule:MF_00247};
GN Name=sthA {ECO:0000255|HAMAP-Rule:MF_00247}; OrderedLocusNames=VF_2439;
OS Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Aliivibrio.
OX NCBI_TaxID=312309;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700601 / ES114;
RX PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with
RT pathogenic congeners.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
CC -!- FUNCTION: Conversion of NADPH, generated by peripheral catabolic
CC pathways, to NADH, which can enter the respiratory chain for energy
CC generation. {ECO:0000255|HAMAP-Rule:MF_00247}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + NADPH = NADH + NADP(+); Xref=Rhea:RHEA:11692,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57783, ChEBI:CHEBI:57945,
CC ChEBI:CHEBI:58349; EC=1.6.1.1; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00247};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00247};
CC Note=Binds 1 FAD per subunit. {ECO:0000255|HAMAP-Rule:MF_00247};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00247}.
CC -!- SIMILARITY: Belongs to the class-I pyridine nucleotide-disulfide
CC oxidoreductase family. {ECO:0000255|HAMAP-Rule:MF_00247}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAW86934.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000020; AAW86934.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_017019516.1; NC_006840.2.
DR RefSeq; YP_205822.1; NC_006840.2.
DR AlphaFoldDB; Q5E212; -.
DR SMR; Q5E212; -.
DR STRING; 312309.VF_2439; -.
DR EnsemblBacteria; AAW86934; AAW86934; VF_2439.
DR KEGG; vfi:VF_2439; -.
DR PATRIC; fig|312309.11.peg.2467; -.
DR eggNOG; COG1249; Bacteria.
DR HOGENOM; CLU_016755_0_0_6; -.
DR OrthoDB; 267896at2; -.
DR Proteomes; UP000000537; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0003957; F:NAD(P)+ transhydrogenase (B-specific) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0045454; P:cell redox homeostasis; IEA:InterPro.
DR GO; GO:0006739; P:NADP metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.390.30; -; 1.
DR Gene3D; 3.50.50.60; -; 2.
DR HAMAP; MF_00247; SthA; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR016156; FAD/NAD-linked_Rdtase_dimer_sf.
DR InterPro; IPR001100; Pyr_nuc-diS_OxRdtase.
DR InterPro; IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
DR InterPro; IPR022962; STH.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR Pfam; PF02852; Pyr_redox_dim; 1.
DR PIRSF; PIRSF000350; Mercury_reductase_MerA; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR SUPFAM; SSF55424; SSF55424; 1.
PE 3: Inferred from homology;
KW Cytoplasm; FAD; Flavoprotein; NAD; NADP; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..471
FT /note="Soluble pyridine nucleotide transhydrogenase"
FT /id="PRO_0000260245"
FT BINDING 41..50
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00247"
SQ SEQUENCE 471 AA; 51674 MW; A9AE438FF166DD15 CRC64;
MTNSTPIRST HFDAIVIGSG PGGEGAAMGL TKANLNVAII EREPSVGGGC THWGTIPSKA
LRHAVSRIIE FNSNPLYCKN NTSLHSTFSD ILGHAKSVID KQTRMRQGFY DRNQCSLIFG
EASFVEKNTV AVTAKDGSIE TYTADKFIIA TGSRPYRPEG INFNHSRVYD SDSILSLKHD
PRHIIIYGAG VIGSEYASIF RGLGVKVDLV NTRDRLLSFL DNEMSDALSY HFWNSGIVTR
NDENFEHIEA NDDGVIMHLE SGKKMKADCI LFANGRTGNT DKLNLSAVGL EADSRGQLKV
NDNYQTDVEH IYAVGDVIGY PSLASAAYDQ GRFTAQAITK GKAEARLIDH IPTGIYTIPE
ISSVGKTEQE LTAAKVPYEV GRSSFKHLAR AQIAGKDIGS LKILFHRETK EILGIHCFGE
RAAEIIHIGQ AIMEQKGEAN TIEYFVNTTF NYPTMAEAYR VAALNGLNRL F
