STIL_DANRE
ID STIL_DANRE Reviewed; 1263 AA.
AC Q8JGS1; Q1LV59; Q58EB7;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=SCL-interrupting locus protein homolog;
GN Name=stil; Synonyms=sill; ORFNames=zgc:110502;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC TISSUE=Embryo;
RX PubMed=12006978; DOI=10.1038/ng896;
RA Golling G., Amsterdam A., Sun Z., Antonelli M., Maldonado E., Chen W.,
RA Burgess S., Haldi M., Artzt K., Farrington S., Lin S.-Y., Nissen R.M.,
RA Hopkins N.;
RT "Insertional mutagenesis in zebrafish rapidly identifies genes essential
RT for early vertebrate development.";
RL Nat. Genet. 31:135-140(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plays an essential role in early embryonic development
CC (PubMed:12006978). Plays an important role in the regulation of
CC centriole duplication (By similarity). {ECO:0000250|UniProtKB:Q15468,
CC ECO:0000269|PubMed:12006978}.
CC -!- INTERACTION:
CC Q8JGS1; E7FCY1: cenpj; NbExp=3; IntAct=EBI-15182936, EBI-15182913;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Cytoplasm, cytoskeleton,
CC microtubule organizing center, centrosome, centriole
CC {ECO:0000250|UniProtKB:Q15468}.
CC -!- DISRUPTION PHENOTYPE: Embryos display defects such as small head, small
CC eyes and dorsally bent body. {ECO:0000269|PubMed:12006978}.
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DR EMBL; AY099525; AAM28213.1; -; mRNA.
DR EMBL; BX908392; CAK05348.1; -; Genomic_DNA.
DR EMBL; BC091991; AAH91991.1; -; mRNA.
DR RefSeq; NP_775351.2; NM_173244.2.
DR PDB; 4BXR; X-ray; 2.20 A; C/D=408-428.
DR PDB; 4LD3; X-ray; 2.44 A; B=399-450.
DR PDB; 4LZF; X-ray; 1.72 A; B=414-428.
DR PDBsum; 4BXR; -.
DR PDBsum; 4LD3; -.
DR PDBsum; 4LZF; -.
DR AlphaFoldDB; Q8JGS1; -.
DR SMR; Q8JGS1; -.
DR ComplexPortal; CPX-1154; CPAP-STIL complex.
DR DIP; DIP-60582N; -.
DR IntAct; Q8JGS1; 1.
DR STRING; 7955.ENSDARP00000037419; -.
DR PaxDb; Q8JGS1; -.
DR PRIDE; Q8JGS1; -.
DR Ensembl; ENSDART00000033617; ENSDARP00000037419; ENSDARG00000024904.
DR Ensembl; ENSDART00000180972; ENSDARP00000146373; ENSDARG00000114288.
DR Ensembl; ENSDART00000181617; ENSDARP00000146321; ENSDARG00000024904.
DR GeneID; 192317; -.
DR KEGG; dre:192317; -.
DR CTD; 6491; -.
DR ZFIN; ZDB-GENE-020419-39; stil.
DR eggNOG; ENOG502QVJ5; Eukaryota.
DR GeneTree; ENSGT00390000007310; -.
DR HOGENOM; CLU_007178_0_0_1; -.
DR InParanoid; Q8JGS1; -.
DR OMA; CTVNAWG; -.
DR OrthoDB; 342602at2759; -.
DR PhylomeDB; Q8JGS1; -.
DR TreeFam; TF331178; -.
DR PRO; PR:Q8JGS1; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 22.
DR Bgee; ENSDARG00000024904; Expressed in early embryo and 26 other tissues.
DR ExpressionAtlas; Q8JGS1; baseline and differential.
DR GO; GO:0005814; C:centriole; ISS:UniProtKB.
DR GO; GO:0005813; C:centrosome; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IC:ComplexPortal.
DR GO; GO:0120099; C:procentriole replication complex; IC:ComplexPortal.
DR GO; GO:0010171; P:body morphogenesis; IMP:ZFIN.
DR GO; GO:0051298; P:centrosome duplication; IBA:GO_Central.
DR GO; GO:0007052; P:mitotic spindle organization; IMP:ZFIN.
DR GO; GO:0070050; P:neuron cellular homeostasis; IMP:ZFIN.
DR GO; GO:0046601; P:positive regulation of centriole replication; IC:ComplexPortal.
DR GO; GO:1900087; P:positive regulation of G1/S transition of mitotic cell cycle; IC:ComplexPortal.
DR GO; GO:0110028; P:positive regulation of mitotic spindle organization; IC:ComplexPortal.
DR GO; GO:1905832; P:positive regulation of spindle assembly; IC:ComplexPortal.
DR GO; GO:0071539; P:protein localization to centrosome; IBA:GO_Central.
DR GO; GO:0046599; P:regulation of centriole replication; ISS:UniProtKB.
DR GO; GO:0007224; P:smoothened signaling pathway; IGI:ZFIN.
DR GO; GO:0051225; P:spindle assembly; IMP:ZFIN.
DR InterPro; IPR026123; Sil.
DR PANTHER; PTHR15128; PTHR15128; 1.
DR Pfam; PF15253; STIL_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Cytoskeleton; Developmental protein;
KW Reference proteome.
FT CHAIN 1..1263
FT /note="SCL-interrupting locus protein homolog"
FT /id="PRO_0000271335"
FT REGION 395..432
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 455..614
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 778..803
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 818..884
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 954..988
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1210..1243
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 455..480
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 509..614
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 818..854
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 867..884
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1215..1233
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 149
FT /note="A -> S (in Ref. 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 234
FT /note="P -> L (in Ref. 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 275
FT /note="S -> I (in Ref. 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 299
FT /note="Q -> L (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 315
FT /note="S -> A (in Ref. 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 344
FT /note="D -> E (in Ref. 1; AAM28213 and 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 357
FT /note="G -> R (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 432
FT /note="R -> G (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 494
FT /note="K -> G (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 612
FT /note="H -> Q (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 675
FT /note="S -> T (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 703..705
FT /note="SGP -> CGR (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 711
FT /note="Missing (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 754
FT /note="I -> M (in Ref. 1; AAM28213 and 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 787
FT /note="Q -> H (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 836
FT /note="T -> I (in Ref. 1; AAM28213 and 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 887
FT /note="M -> I (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 953
FT /note="T -> R (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 960..961
FT /note="VH -> GQ (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 962
FT /note="Q -> E (in Ref. 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 968
FT /note="T -> I (in Ref. 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 985
FT /note="P -> S (in Ref. 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 1111
FT /note="P -> S (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 1163
FT /note="G -> R (in Ref. 1; AAM28213 and 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 1182
FT /note="V -> I (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
FT CONFLICT 1211
FT /note="G -> R (in Ref. 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 1223
FT /note="N -> D (in Ref. 1; AAM28213 and 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 1226
FT /note="E -> Q (in Ref. 3; AAH91991)"
FT /evidence="ECO:0000305"
FT CONFLICT 1235
FT /note="Q -> R (in Ref. 1; AAM28213)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1263 AA; 138246 MW; F8C0FFFA169927F4 CRC64;
MNRVQVDFKG LPAHILENSI AAESLQNTRS SDNVLTPLTF PKSKVALWDP SANGEVVSLH
FSYYRNPRLF LVEKALRLAH RHARQTNKPR FFCFLLGTLA VDSDEEGVTI TLDRFDPGRE
QTGCLGKAPT ALLPGDILVP CVFEAQHAAS STVHSSEDLN ISFKMLQHFC CSKELLELSK
LLTLRAQLSC SENMDRLTFN LSWAAVTLAC TLDAVPIRAV PIIPTALARN LSSPAGVTQN
SKRGFLTMDQ TRKLLLILES DPKAYTLPLV GIWLSGVTHI HNPLVWAWCL RYLHSSSLQD
KVLSEGGTFL VVLYSLTHRD PEFYQCKPST GQQQLSFQLL TSRDSLTLYK NVEPSEGRPL
QFELSSENQN QETVLFEEVL SQSVLTGTTL GAASAAPQNK LSISDHDSGV EDEDLSPRPS
PNPHPVSQQT KRVHPLVPEL SMVLDGSFLD GSVVNTQGST PLSHSQSNVH RRNSSPALQG
LSVLRPLVQG SVTKPPPIRR PLTPILSQPK NKLHPNPSQQ TPQHSVSRKS LPSMRRSREG
SSASSVSSSS SSSSTKNASP NGSFHQQRQR LSQGFPNKPQ LIYSGPPTSG HSSAKKSSSV
PSQTPVPHPS QHRIFHSTPA VNPCNCCTNH PSVAPLYQNN TWQGTPGYPT AVHSPCVFHC
SPETVPPGDH CLSPSRQSLG CRVSPTKSPV CYHSTPPHYS PSSGPCVPTI ISNKGLVEQT
PSCQAQCCQV KGSKEPCLDT PMGLLPADAY RMLIDQERQL KLLQLQIQKL LESQSKVPEV
SSEQNAQQQR PNQVPASPPK RTSVSIAVGT GASLFWSTPQ ETSTHEASSL EWQTETEPKS
GCQNDSTVTS RDRSESACHY SEEHCPGSPQ HPTSPQHNTS SGFGVQMFQS PVLGESASMY
YQSQSQSKDL SENREIDDPR FYHELLGQVQ SRLQDSVIVE DKVEQDQQSL LKTQSLSPVV
HQSRKPLTTS SIPQTQKTKQ PSSPPNQDRV LSATLKQLQQ FGVNIDLDSS QEKTTRATVE
SASTLACINP EAVIPRLALS EPVGASIWGP SGSVDLSLEA NAIALKYLSD SQLSRLSLGS
QSSSPHSDPS TILLRRPAVE KSNVALSILS PSNMSLATCK YMKKYGLIEG EISSEEEQED
PIQVDSALGC SVQHETSKTI SLGQEREEQN TAVLKNITNK PVVNLHTSPI DSQEQILQDL
RPKMQLLLRG GTNSEKENAT KRNLIERRSS LTENQRTQEV VDPQGSVGNF LDLSRLRQLP
KLF
