STIL_DROME
ID STIL_DROME Reviewed; 321 AA.
AC P92189; Q9V6D8;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Protein stand still;
GN Name=stil; ORFNames=CG8592;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Ovary;
RX PubMed=9093851; DOI=10.1093/genetics/145.4.975;
RA Pennetta G., Pauli D.;
RT "stand still, a Drosophila gene involved in the female germline for proper
RT survival, sex determination and differentiation.";
RL Genetics 145:975-987(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MUTANTS STIL-1;
RP STIL-2 AND STIL-4.
RX PubMed=10101125; DOI=10.1242/dev.126.9.1917;
RA Sahut-Barnola I., Pauli D.;
RT "The Drosophila gene stand still encodes a germline chromatin-associated
RT protein that controls the transcription of the ovarian tumor gene.";
RL Development 126:1917-1926(1999).
CC -!- FUNCTION: Essential in the female germline for proper survival, sex
CC determination and differentiation. Participates in the transcriptional
CC activation of Otu. Does not regulate the expression of Ovo.
CC {ECO:0000269|PubMed:10101125, ECO:0000269|PubMed:9093851}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10101125}.
CC Note=Associates with decondensed chromatin, including sites of active
CC transcription.
CC -!- TISSUE SPECIFICITY: Germ cells specific. Expressed in all germ cells.
CC During the first instar larvae, it is expressed in all germ cells of
CC both sexes. In third instar larvae, it decreases in male germ cells
CC while it remains in female germ cells. In adult ovary, it is expressed
CC in cells of the germarium, including the stem cells. In the early
CC previtellogenic stages, it is highly expressed in the nurse cells.
CC During vitellogenesis, it is not translocated into the maturing egg. In
CC testes, it is only expressed during some steps of male germline
CC differentiation. At the apex testis, it is expressed at low level in
CC stem cells and dividing spermatogonia, while in newly formed 16-cell
CC cysts of primary spermatocytes, it is transiently but strongly
CC expressed before vanishing during spermatocyte growth phase.
CC {ECO:0000269|PubMed:10101125, ECO:0000269|PubMed:9093851}.
CC -!- DEVELOPMENTAL STAGE: In embryos, it is expressed from stage 11 in the
CC germ cell soon after their migration through the midgut epithelium.
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DR EMBL; Y10276; CAA71319.1; -; Genomic_DNA.
DR EMBL; Y10277; CAA71320.1; -; mRNA.
DR EMBL; AE013599; AAF58490.1; -; Genomic_DNA.
DR EMBL; AY075316; AAL68183.1; -; mRNA.
DR RefSeq; NP_476752.1; NM_057404.4.
DR AlphaFoldDB; P92189; -.
DR BioGRID; 62154; 1.
DR DIP; DIP-23414N; -.
DR IntAct; P92189; 2.
DR STRING; 7227.FBpp0086938; -.
DR PaxDb; P92189; -.
DR DNASU; 36380; -.
DR EnsemblMetazoa; FBtr0087825; FBpp0086938; FBgn0003527.
DR GeneID; 36380; -.
DR KEGG; dme:Dmel_CG8592; -.
DR CTD; 6491; -.
DR FlyBase; FBgn0003527; stil.
DR VEuPathDB; VectorBase:FBgn0003527; -.
DR eggNOG; ENOG502T7X3; Eukaryota.
DR HOGENOM; CLU_880734_0_0_1; -.
DR InParanoid; P92189; -.
DR OMA; MVSIPRM; -.
DR OrthoDB; 1020413at2759; -.
DR PhylomeDB; P92189; -.
DR BioGRID-ORCS; 36380; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36380; -.
DR PRO; PR:P92189; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0003527; Expressed in testis and 11 other tissues.
DR Genevisible; P92189; DM.
DR GO; GO:0000785; C:chromatin; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003682; F:chromatin binding; IDA:UniProtKB.
DR GO; GO:0030237; P:female sex determination; IMP:UniProtKB.
DR GO; GO:0046660; P:female sex differentiation; IMP:UniProtKB.
DR GO; GO:0018992; P:germ-line sex determination; IMP:UniProtKB.
DR GO; GO:0048477; P:oogenesis; HMP:FlyBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..321
FT /note="Protein stand still"
FT /id="PRO_0000072274"
FT REGION 146..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 227..248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 74..103
FT /evidence="ECO:0000255"
FT COILED 147..167
FT /evidence="ECO:0000255"
FT COILED 272..292
FT /evidence="ECO:0000255"
FT COMPBIAS 146..162
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 230..248
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 19
FT /note="P->S: In stil-4; induces female infertility due to
FT germ ovary defects. Loss of chromatin association."
FT MUTAGEN 58
FT /note="G->R: In stil-2; induces female infertility due to
FT germ ovary defects. Loss of chromatin association."
FT MUTAGEN 79
FT /note="K->M: In stil-1; induces female infertility due to
FT germ ovary defects."
FT CONFLICT 216
FT /note="L -> M (in Ref. 1; CAA71319/CAA71320)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 321 AA; 35776 MW; 759D9DD97017DE77 CRC64;
MSVNLDGSEE KMELKKHVPY ILNGELYRIE KQVGDNVTVK CCYCPPDRIY RGSVRSTGNF
HMHIKRRHSS LLGKLHEMKV AALEERRDRI MKNRRFAKSR KKAPVAVAAT STAAQSDSGV
FVDMQAAVPS GNESHELKIK TVFQRHKQEQ EGATRKLEDS TSDKANLPNI PKNLGIVVQN
VSNISVETLP GGSTPASVSF LGRPVKPEQG SGPSFLADQP AAIDLSQVPP VQGESKSSGS
LASSMEDVSM EYSRSQALSQ SLSMAHFLEH PQRDVLQRLE RSMAQISQEL HCRNRIEHNR
MLLEAAKFKF LNPNFQFEPN L
