STIP2_ARATH
ID STIP2_ARATH Reviewed; 752 AA.
AC Q9SLC6;
DT 11-JUN-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Septin and tuftelin-interacting protein 1 homolog 2;
DE AltName: Full=Protein SPLICEOSOMAL TIMEKEEPER LOCUS 2;
GN Name=STIPL2; OrderedLocusNames=At2g42330;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 203-752.
RC STRAIN=cv. Columbia;
RX PubMed=12481096; DOI=10.1104/pp.010207;
RA Xiao Y.-L., Malik M., Whitelaw C.A., Town C.D.;
RT "Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2
RT of Arabidopsis.";
RL Plant Physiol. 130:2118-2128(2002).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=23110899; DOI=10.1105/tpc.112.104828;
RA Jones M.A., Williams B.A., McNicol J., Simpson C.G., Brown J.W.,
RA Harmer S.L.;
RT "Mutation of Arabidopsis spliceosomal timekeeper locus1 causes circadian
RT clock defects.";
RL Plant Cell 24:4066-4082(2012).
CC -!- FUNCTION: Involved in pre-mRNA splicing, specifically in spliceosome
CC disassembly during late-stage splicing events. {ECO:0000250}.
CC -!- SUBUNIT: Identified in the spliceosome C complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. {ECO:0000269|PubMed:23110899}.
CC -!- SIMILARITY: Belongs to the TFP11/STIP family. {ECO:0000305}.
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DR EMBL; AC005956; AAD23716.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10107.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10108.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62879.1; -; Genomic_DNA.
DR EMBL; AY429349; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; F84852; F84852.
DR RefSeq; NP_001078041.1; NM_001084572.3.
DR RefSeq; NP_001325005.1; NM_001336968.1.
DR RefSeq; NP_181762.1; NM_129795.4.
DR AlphaFoldDB; Q9SLC6; -.
DR SMR; Q9SLC6; -.
DR STRING; 3702.AT2G42330.2; -.
DR PaxDb; Q9SLC6; -.
DR PRIDE; Q9SLC6; -.
DR ProteomicsDB; 228266; -.
DR EnsemblPlants; AT2G42330.1; AT2G42330.1; AT2G42330.
DR EnsemblPlants; AT2G42330.2; AT2G42330.2; AT2G42330.
DR EnsemblPlants; AT2G42330.3; AT2G42330.3; AT2G42330.
DR GeneID; 818834; -.
DR Gramene; AT2G42330.1; AT2G42330.1; AT2G42330.
DR Gramene; AT2G42330.2; AT2G42330.2; AT2G42330.
DR Gramene; AT2G42330.3; AT2G42330.3; AT2G42330.
DR KEGG; ath:AT2G42330; -.
DR Araport; AT2G42330; -.
DR TAIR; locus:2053839; AT2G42330.
DR eggNOG; KOG2184; Eukaryota.
DR HOGENOM; CLU_007977_1_0_1; -.
DR InParanoid; Q9SLC6; -.
DR OMA; DIWEDAT; -.
DR OrthoDB; 1238995at2759; -.
DR PhylomeDB; Q9SLC6; -.
DR PRO; PR:Q9SLC6; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SLC6; baseline and differential.
DR Genevisible; Q9SLC6; AT.
DR GO; GO:0071008; C:U2-type post-mRNA release spliceosomal complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IDA:TAIR.
DR GO; GO:0006468; P:protein phosphorylation; IDA:TAIR.
DR GO; GO:0010600; P:regulation of auxin biosynthetic process; IDA:TAIR.
DR GO; GO:0000390; P:spliceosomal complex disassembly; IBA:GO_Central.
DR InterPro; IPR000467; G_patch_dom.
DR InterPro; IPR022783; GCFC_dom.
DR InterPro; IPR022159; STIP/TFIP11_N.
DR InterPro; IPR045211; TFP11/STIP/Ntr1.
DR PANTHER; PTHR23329; PTHR23329; 1.
DR Pfam; PF01585; G-patch; 1.
DR Pfam; PF07842; GCFC; 1.
DR Pfam; PF12457; TIP_N; 1.
DR SMART; SM00443; G_patch; 1.
DR PROSITE; PS50174; G_PATCH; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Spliceosome.
FT CHAIN 1..752
FT /note="Septin and tuftelin-interacting protein 1 homolog 2"
FT /id="PRO_0000429431"
FT DOMAIN 103..148
FT /note="G-patch"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00092"
FT REGION 1..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 36..48
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 1..17
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 268
FT /note="D -> V (in Ref. 3; AY429349)"
FT /evidence="ECO:0000305"
FT CONFLICT 331
FT /note="K -> E (in Ref. 3; AY429349)"
FT /evidence="ECO:0000305"
FT CONFLICT 564
FT /note="S -> P (in Ref. 3; AY429349)"
FT /evidence="ECO:0000305"
FT CONFLICT 744
FT /note="H -> R (in Ref. 3; AY429349)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 752 AA; 86525 MW; F11A99B68B16D163 CRC64;
MGDEGKKEKR RQTKEDAVYG FVFESDSNSD DSGGSRRKKR RKTKPVKFSS AGNIDQVLKQ
NRGNCKIDEN DDTILPIALG KKIADKAHVR EKNNKKENFE KFSGGIGMKL LEKMGYKGRG
LGKNQQGIVA PIEVQLRPKN MGMGYNDFKE KNAPLFPCLN KVEEKKKSVV VTVSENHGDG
RRDLWKKKNV RKEVYITAEE FLGKKQEEGF GCDQLIIDKR GPQDRVVNSL RNLYAEEKAT
DANVQQPELQ HNLRFIVKSL EHGILKTDKD LRNEKGLALS LQQEKEKFKM GVKKQKTLFD
NLGYVAEEID RIEVEIASGN LTLDSLANRF KDLRSSYPDD YKCCNLSCIA SSLALPLFIR
MFQGWDPLSD AEHGIEAISS WKMLLEVEDN QSISTPYSQL VSEVILPAVR VSGINTWEPR
DPEPMLRLLE TWEKMLPSLI FETILTTVVL PKLSIAIESW EPRLETVPIH FWVHPWLPVL
GQKLESAYQI IRMKFGNLLD AWHPSDVSVH TILSPWKTVF DAASWEQLMR RYIVPKLQVA
LQEFQINPAD QNLDEFNLVM GWVSSVPIHL MTDLMERFFF PKWLDVLYHW LCSEPKFDEI
MKWFLGWKGT FPQELSANRR IEIQFKRGLD MAREAVERME MSQPGARENI SYHKAQEQRQ
SEGRAKVQAQ VDDPEELSFK EAVELFAQEK ELLLKPKPHR MHNGLQIYRF GNVSVLLDSA
NSKLLAQEEG RWFPVDLDSL LKMHYSAVTG KQ
