STI_ARATH
ID STI_ARATH Reviewed; 1218 AA.
AC O64728; Q9LKQ4;
DT 24-JUL-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Protein STICHEL;
GN Name=STI; OrderedLocusNames=At2g02480; ORFNames=T8K22.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Marks M.D., Prigge M., Shi H.;
RT "The Arabidopsis STI gene is a member of the replication factor C-like gene
RT family.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP DISRUPTION PHENOTYPE, AND MUTANT STICHEL.
RX PubMed=8313475; DOI=10.1016/0092-8674(94)90118-x;
RA Huelskamp M., Misra S., Juergens G.;
RT "Genetic dissection of trichome cell development in Arabidopsis.";
RL Cell 76:555-566(1994).
RN [5]
RP FUNCTION.
RX PubMed=9367433; DOI=10.1242/dev.124.19.3779;
RA Folkers U., Berger J., Huelskamp M.;
RT "Cell morphogenesis of trichomes in Arabidopsis: differential control of
RT primary and secondary branching by branch initiation regulators and cell
RT growth.";
RL Development 124:3779-3786(1997).
RN [6]
RP FUNCTION.
RX PubMed=10572032; DOI=10.1242/dev.126.24.5547;
RA Luo D., Oppenheimer D.G.;
RT "Genetic control of trichome branch number in Arabidopsis: the roles of the
RT FURCA loci.";
RL Development 126:5547-5557(1999).
RN [7]
RP GENE FAMILY, PEST MOTIF, NUCLEAR LOCALIZATION SIGNAL, DISRUPTION PHENOTYPE,
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12586888; DOI=10.1104/pp.014209;
RA Ilgenfritz H., Bouyer D., Schnittger A., Mathur J., Kirik V., Schwab B.,
RA Chua N.H., Juergens G., Huelskamp M.;
RT "The Arabidopsis STICHEL gene is a regulator of trichome branch number and
RT encodes a novel protein.";
RL Plant Physiol. 131:643-655(2003).
RN [8]
RP FUNCTION.
RX PubMed=18477400; DOI=10.1186/1471-2229-8-54;
RA Exner V., Gruissem W., Hennig L.;
RT "Control of trichome branching by chromatin assembly factor-1.";
RL BMC Plant Biol. 8:54-54(2008).
RN [9]
RP INTERACTION WITH BLT.
RX PubMed=21558384; DOI=10.1242/dev.058982;
RA Kasili R., Huang C.C., Walker J.D., Simmons L.A., Zhou J., Faulk C.,
RA Huelskamp M., Larkin J.C.;
RT "BRANCHLESS TRICHOMES links cell shape and cell cycle control in
RT Arabidopsis trichomes.";
RL Development 138:2379-2388(2011).
RN [10]
RP INDUCTION BY GA3, AND FUNCTION.
RX PubMed=22210898; DOI=10.1093/pcp/pcr192;
RA An L., Zhou Z., Su S., Yan A., Gan Y.;
RT "GLABROUS INFLORESCENCE STEMS (GIS) is required for trichome branching
RT through gibberellic acid signaling in Arabidopsis.";
RL Plant Cell Physiol. 53:457-469(2012).
CC -!- FUNCTION: Acts as a key regulator of trichome branching through an
CC endoreduplication-independent pathway. {ECO:0000269|PubMed:10572032,
CC ECO:0000269|PubMed:12586888, ECO:0000269|PubMed:18477400,
CC ECO:0000269|PubMed:22210898, ECO:0000269|PubMed:9367433}.
CC -!- SUBUNIT: Interacts with BLT. {ECO:0000269|PubMed:21558384}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:12586888}.
CC -!- INDUCTION: By gibberellin A3 (GA3). {ECO:0000269|PubMed:22210898}.
CC -!- DOMAIN: PEST motif is known to mediate rapid protein degradation.
CC -!- DISRUPTION PHENOTYPE: Shows trichomes with exclusively no branching.
CC {ECO:0000269|PubMed:12586888, ECO:0000269|PubMed:8313475}.
CC -!- SIMILARITY: Belongs to the DnaX/STICHEL family. {ECO:0000305}.
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DR EMBL; AF264023; AAF82285.1; -; mRNA.
DR EMBL; AC004136; AAC18938.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC05585.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62557.1; -; Genomic_DNA.
DR PIR; T00615; T00615.
DR RefSeq; NP_001324706.1; NM_001335120.1.
DR RefSeq; NP_565285.1; NM_126303.3.
DR AlphaFoldDB; O64728; -.
DR SMR; O64728; -.
DR BioGRID; 180; 2.
DR IntAct; O64728; 1.
DR STRING; 3702.AT2G02480.1; -.
DR iPTMnet; O64728; -.
DR PaxDb; O64728; -.
DR PRIDE; O64728; -.
DR ProteomicsDB; 228423; -.
DR EnsemblPlants; AT2G02480.1; AT2G02480.1; AT2G02480.
DR EnsemblPlants; AT2G02480.2; AT2G02480.2; AT2G02480.
DR GeneID; 814777; -.
DR Gramene; AT2G02480.1; AT2G02480.1; AT2G02480.
DR Gramene; AT2G02480.2; AT2G02480.2; AT2G02480.
DR KEGG; ath:AT2G02480; -.
DR Araport; AT2G02480; -.
DR TAIR; locus:2065299; AT2G02480.
DR eggNOG; KOG0989; Eukaryota.
DR HOGENOM; CLU_009072_0_0_1; -.
DR InParanoid; O64728; -.
DR OMA; FMPSIRR; -.
DR OrthoDB; 97895at2759; -.
DR PhylomeDB; O64728; -.
DR PRO; PR:O64728; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64728; baseline and differential.
DR Genevisible; O64728; AT.
DR GO; GO:0009360; C:DNA polymerase III complex; IEA:InterPro.
DR GO; GO:0005663; C:DNA replication factor C complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0006261; P:DNA-templated DNA replication; IBA:GO_Central.
DR GO; GO:0010091; P:trichome branching; IMP:UniProtKB.
DR GO; GO:0010026; P:trichome differentiation; IMP:TAIR.
DR CDD; cd18137; HLD_clamp_pol_III_gamma_tau; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR008921; DNA_pol3_clamp-load_cplx_C.
DR InterPro; IPR022754; DNA_pol_III_gamma-3.
DR InterPro; IPR012763; DNA_pol_III_sug/sutau_N.
DR InterPro; IPR045085; HLD_clamp_pol_III_gamma_tau.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF12169; DNA_pol3_gamma3; 1.
DR SUPFAM; SSF48019; SSF48019; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02397; dnaX_nterm; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Coiled coil; Nucleotide-binding; Nucleus; Reference proteome;
KW Repeat.
FT CHAIN 1..1218
FT /note="Protein STICHEL"
FT /id="PRO_0000422976"
FT REGION 24..136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 802..828
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 762..788
FT /evidence="ECO:0000255"
FT MOTIF 163..180
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 273..304
FT /note="PEST 1"
FT MOTIF 425..449
FT /note="PEST 2"
FT MOTIF 1178..1195
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 1196..1213
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 32..70
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 490..497
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1218 AA; 135303 MW; 123D73BD39965CC5 CRC64;
MSGSRVSDLS KLHLKKELTQ IRKAGRVLRD PGTTSSWKSP LDSSRSVALL ETPASRNGGS
SSQFPIRGES STNRRGKEKK VFLYNWKTQK SSSEKSGLAK NGKEEEEEEE DASSWTQASV
NDDDDVSDAR NGGDSYRREI QSASMGFRCR DTNLASQGVS KMRKSNVGSC KKKSKKKISS
SRLDCLSKYQ PRDDIVARNC NAGSDDTEEE LSNSEDLRKV TGASPLLLKL KQKNWSRSSS
RLLRANNRKE DSSCTYNSTP ALSTSSYNMY AVRNPSTVGS WDGTTTSVND GDDELDDNLD
LPGRQGCGIP CYWTKKAMKH RGGCRSCCSP SFSDTLRRTG SSILCGSQSV YRRHNRHSSG
GYSKQKIACR SAQGVLPLLS YGGDGRGGSS LGTGLSDDEL STNYGELDLE AQSRLDGRRW
STSYRSQDGL EAVALDGEEE EGSTPETIRS FSQKYRPMFF EELIGQSIVV QSLMNAVKRS
RIAPVYLFQG PRGTGKTSTA RIFSAALNCV ATEEMKPCGY CKECNDFMSG KSKDFWELDG
ANKKGADKVR YLLKNLPTIL PRNSSMYKVF VIDECHLLPS KTWLSFLKFL ENPLQKVVFI
FITTDLENVP RTIQSRCQKF LFDKLKDSDI VVRLKKIASD ENLDVDLHAL DLIAMNADGS
LRDAETMLEQ LSLLGKRITT ALVNELVGVV SDEKLLELLE LALSSDTAET VKRARELLDL
GADPIVLMSQ LASLIMDIIA GTYKVVDEKY SNAFFDGRNL TEADMEGLKH ALKLLSEAEK
QLRVSNDRST WFTATLLQLG SMPSPGTTHT GSSRRQSSRA TDDDPASVSR EVMAYKQRIG
GLHFSKSASP ASVIKRNGNH SHEAKPFSRV IDNNCYKSSS SSQMIESEGS IASHENSIAS
TMMLNQRSSE KLNDIWRKCI ERCHSKTLRQ LLYTHGKLIS ISEVEGILVA YIAFGENDIK
LRAERFLSSI TNSIEMVLRR SVEVRIILLP ETELLVVPHQ TRKPEMTNKS GHLNNIAGLN
AETDVEVGSS VESRSKLPMQ RIESIIREQR LETAWLQTAD KDTPGSIIRV KPERNQILPQ
EDTYRQTNVA SAISSSGLTT HQWVDELNNE VKLLKIGDNG ELQENLTGTR GQHCPLSPSL
LHDTNFGNNK DNLGGYESGS GRVGCNILFC WNTKKTQRRS KSKQVKGTPV RSRRNRKSRF
SLFNGCAKPR KAEGNIRR
