STKL1_HUMAN
ID STKL1_HUMAN Reviewed; 680 AA.
AC Q8NE28; Q5T8U8; Q6ZMP6; Q6ZMQ5;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2018, sequence version 5.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Serine/threonine kinase-like domain-containing protein STKLD1;
DE AltName: Full=Serine/threonine kinase-like domain-containing protein 1;
DE AltName: Full=Sugen kinase 071;
GN Name=STKLD1; Synonyms=C9orf96, SGK071;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC TISSUE=Amygdala, and Thalamus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION.
RX PubMed=12471243; DOI=10.1126/science.1075762;
RA Manning G., Whyte D.B., Martinez R., Hunter T., Sudarsanam S.;
RT "The protein kinase complement of the human genome.";
RL Science 298:1912-1934(2002).
RN [5]
RP VARIANT [LARGE SCALE ANALYSIS] ASP-139.
RX PubMed=17344846; DOI=10.1038/nature05610;
RA Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G.,
RA Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S.,
RA Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.,
RA Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K.,
RA Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D.,
RA Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R.,
RA Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A.,
RA Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F.,
RA Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F.,
RA Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G.,
RA Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R.,
RA Futreal P.A., Stratton M.R.;
RT "Patterns of somatic mutation in human cancer genomes.";
RL Nature 446:153-158(2007).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8NE28-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8NE28-2; Sequence=VSP_021658, VSP_021659;
CC Name=3;
CC IsoId=Q8NE28-3; Sequence=VSP_021660, VSP_021657;
CC -!- DOMAIN: The protein kinase domain is predicted to be catalytically
CC inactive.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. STKL subfamily. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- CAUTION: Asn-157 is present instead of the conserved Asp which is
CC expected to be an active site residue. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAI12843.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AK131533; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK131545; BAD18679.1; -; mRNA.
DR EMBL; AL158826; CAI12843.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL593848; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC036504; AAH36504.3; -; mRNA.
DR CCDS; CCDS35169.1; -. [Q8NE28-1]
DR RefSeq; NP_714921.4; NM_153710.4. [Q8NE28-1]
DR AlphaFoldDB; Q8NE28; -.
DR SMR; Q8NE28; -.
DR BioGRID; 127981; 5.
DR STRING; 9606.ENSP00000361025; -.
DR iPTMnet; Q8NE28; -.
DR PhosphoSitePlus; Q8NE28; -.
DR BioMuta; STKLD1; -.
DR DMDM; 90111984; -.
DR EPD; Q8NE28; -.
DR jPOST; Q8NE28; -.
DR MassIVE; Q8NE28; -.
DR PaxDb; Q8NE28; -.
DR PeptideAtlas; Q8NE28; -.
DR PRIDE; Q8NE28; -.
DR ProteomicsDB; 73118; -. [Q8NE28-1]
DR ProteomicsDB; 73119; -. [Q8NE28-2]
DR ProteomicsDB; 73120; -. [Q8NE28-3]
DR Antibodypedia; 31860; 212 antibodies from 23 providers.
DR DNASU; 169436; -.
DR Ensembl; ENST00000371957.4; ENSP00000361025.3; ENSG00000198870.8. [Q8NE28-1]
DR Ensembl; ENST00000627962.2; ENSP00000486786.1; ENSG00000281245.2. [Q8NE28-1]
DR GeneID; 169436; -.
DR KEGG; hsa:169436; -.
DR MANE-Select; ENST00000371957.4; ENSP00000361025.3; NM_153710.5; NP_714921.4.
DR UCSC; uc033dkl.2; human. [Q8NE28-1]
DR CTD; 169436; -.
DR DisGeNET; 169436; -.
DR GeneCards; STKLD1; -.
DR HGNC; HGNC:28669; STKLD1.
DR HPA; ENSG00000198870; Tissue enriched (testis).
DR MIM; 618530; gene.
DR neXtProt; NX_Q8NE28; -.
DR OpenTargets; ENSG00000198870; -.
DR PharmGKB; PA134891744; -.
DR VEuPathDB; HostDB:ENSG00000198870; -.
DR eggNOG; KOG0589; Eukaryota.
DR GeneTree; ENSGT00390000018038; -.
DR HOGENOM; CLU_013402_0_0_1; -.
DR InParanoid; Q8NE28; -.
DR OMA; ILDMTSC; -.
DR OrthoDB; 268303at2759; -.
DR PhylomeDB; Q8NE28; -.
DR TreeFam; TF336364; -.
DR PathwayCommons; Q8NE28; -.
DR SignaLink; Q8NE28; -.
DR BioGRID-ORCS; 169436; 10 hits in 1098 CRISPR screens.
DR ChiTaRS; STKLD1; human.
DR GenomeRNAi; 169436; -.
DR Pharos; Q8NE28; Tdark.
DR PRO; PR:Q8NE28; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q8NE28; protein.
DR Bgee; ENSG00000198870; Expressed in right testis and 95 other tissues.
DR Genevisible; Q8NE28; HS.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR Pfam; PF00069; Pkinase; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Nucleotide-binding; Reference proteome.
FT CHAIN 1..680
FT /note="Serine/threonine kinase-like domain-containing
FT protein STKLD1"
FT /id="PRO_0000227568"
FT DOMAIN 28..297
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 658..680
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 34..42
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 57
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT VAR_SEQ 1..375
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_021658"
FT VAR_SEQ 133..226
FT /note="WMQNVLGQVLDALEYLHHLDIIHRNLKPSNIILISSDHCKLQDLSSNVLMTD
FT KAKWNIRAEEDPFRKSWMAPEALNFSFSQKSDIWSLGCIILD -> IKTEGVPNTRRFP
FT VGDHDSRLFCRSLKFLMDSSGMLPLLSGLLPSACCCEPRGAAACTSKSCVHCLLPLCCL
FT IALQAVYLVSFGLLPVWGYCE (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_021660"
FT VAR_SEQ 227..680
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_021657"
FT VAR_SEQ 400..491
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_021659"
FT VARIANT 139
FT /note="G -> D (in a glioblastoma multiforme sample; somatic
FT mutation)"
FT /evidence="ECO:0000269|PubMed:17344846"
FT /id="VAR_041371"
FT VARIANT 473
FT /note="E -> K (in dbSNP:rs3124747)"
FT /id="VAR_025611"
FT VARIANT 568
FT /note="R -> Q (in dbSNP:rs17150554)"
FT /id="VAR_025612"
SQ SEQUENCE 680 AA; 75677 MW; F4A7477B98900EA8 CRC64;
MLGPGSNRRR PTQGERGPGS PGEPMEKYQV LYQLNPGALG VNLVVEEMET KVKHVIKQVE
CMDDHYASQA LEELMPLLKL RHAHISVYQE LFITWNGEIS SLYLCLVMEF NELSFQEVIE
DKRKAKKIID SEWMQNVLGQ VLDALEYLHH LDIIHRNLKP SNIILISSDH CKLQDLSSNV
LMTDKAKWNI RAEEDPFRKS WMAPEALNFS FSQKSDIWSL GCIILDMTSC SFMDGTEAMH
LRKSLRQSPG SLKAVLKTME EKQIPDVETF RNLLPLMLQI DPSDRITIKD VVHITFLRGS
FKSSCVSLTL HRQMVPASIT DMLLEGNVAS ILEVMQKFSG WPEVQLRAMK RLLKMPADQL
GLPWPPELVE VVVTTMELHD RVLDVQLCAC SLLLHLLGQA LVHHPEAKAP CNQAITSTLL
SALQSHPEEE PLLVMVYSLL AITTTQESES LSEELQNAGL LEHILEHLNS SLESRDVCAS
GLGLLWALLL DGIIVNKAPL EKVPDLISQV LATYPADGEM AEASCGVFWL LSLLGCIKEQ
QFEQVVALLL QSIRLCQDRA LLVNNAYRGL ASLVKVSELA AFKVVVQEEG GSGLSLIKET
YQLHRDDPEV VENVGMLLVH LASYEEILPE LVSSSMKALL QEIKERFTSS LVSDSSAFSK
PGLPPGGSPQ LGCTTSGGLE
