STN1_YEAST
ID STN1_YEAST Reviewed; 494 AA.
AC P38960; D6VS69;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Protein STN1;
GN Name=STN1; OrderedLocusNames=YDR082W; ORFNames=D4456, YD8554.15;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=9042864; DOI=10.1101/gad.11.4.512;
RA Grandin N., Reed S.I., Charbonneau M.;
RT "Stn1, a new Saccharomyces cerevisiae protein, is implicated in telomere
RT size regulation in association with Cdc13.";
RL Genes Dev. 11:512-527(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=7483840; DOI=10.1002/yea.320110708;
RA Coster F., Jonniaux J.-L., Goffeau A.;
RT "Analysis of a 32.8 kb segment of yeast chromosome IV reveals 21 open
RT reading frames, including TPS2, PPH3, RAD55, SED1, PDC2, AFR1, SSS1, SLU7
RT and a tRNA for arginine.";
RL Yeast 11:673-679(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP FUNCTION, AND INTERACTION WITH TEN1.
RX PubMed=11230140; DOI=10.1093/emboj/20.5.1173;
RA Grandin N., Damon C., Charbonneau M.;
RT "Ten1 functions in telomere end protection and length regulation in
RT association with Stn1 and Cdc13.";
RL EMBO J. 20:1173-1183(2001).
RN [7]
RP INTERACTION WITH CDC13.
RX PubMed=11230149; DOI=10.1101/gad.861001;
RA Chandra A., Hughes T.R., Nugent C.I., Lundblad V.;
RT "Cdc13 both positively and negatively regulates telomere replication.";
RL Genes Dev. 15:404-414(2001).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 311-494, OB FOLD DNA-BINDING,
RP SUBUNIT, WHTH REGIONS, AND MUTAGENESIS OF ASP-397 AND TRP-466.
RX PubMed=20008938; DOI=10.1101/gad.1851909;
RA Sun J., Yu E.Y., Yang Y., Confer L.A., Sun S.H., Wan K., Lue N.F., Lei M.;
RT "Stn1-Ten1 is an Rpa2-Rpa3-like complex at telomeres.";
RL Genes Dev. 23:2900-2914(2009).
CC -!- FUNCTION: Has a role in telomere length regulation and telomere end
CC protection. Acts as an inhibitor of telomerase loading through its
CC interaction with CDC13. {ECO:0000269|PubMed:11230140,
CC ECO:0000269|PubMed:9042864}.
CC -!- SUBUNIT: Interacts with CDC13 and TEN1. {ECO:0000269|PubMed:11230140,
CC ECO:0000269|PubMed:11230149, ECO:0000269|PubMed:20008938}.
CC -!- INTERACTION:
CC P38960; P32797: CDC13; NbExp=10; IntAct=EBI-18427, EBI-4187;
CC P38960; P38121: POL12; NbExp=2; IntAct=EBI-18427, EBI-6111;
CC P38960; Q07921: TEN1; NbExp=12; IntAct=EBI-18427, EBI-35131;
CC -!- SUBCELLULAR LOCATION: Chromosome, telomere {ECO:0000305}.
CC -!- DOMAIN: The C-terminal wHLH region seems to mediate telomere-specific
CC function.
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DR EMBL; AF002132; AAB60885.1; -; Genomic_DNA.
DR EMBL; X82086; CAA57609.1; -; Genomic_DNA.
DR EMBL; Z46796; CAA86804.1; -; Genomic_DNA.
DR EMBL; Z74378; CAA98902.1; -; Genomic_DNA.
DR EMBL; AY723774; AAU09691.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11929.1; -; Genomic_DNA.
DR PIR; S48769; S48769.
DR RefSeq; NP_010367.1; NM_001180390.1.
DR PDB; 3K10; X-ray; 2.50 A; A=313-491.
DR PDB; 3KEY; X-ray; 2.10 A; A=311-494.
DR PDBsum; 3K10; -.
DR PDBsum; 3KEY; -.
DR AlphaFoldDB; P38960; -.
DR SMR; P38960; -.
DR BioGRID; 32138; 259.
DR ComplexPortal; CPX-15; CST complex.
DR DIP; DIP-1230N; -.
DR IntAct; P38960; 6.
DR MINT; P38960; -.
DR STRING; 4932.YDR082W; -.
DR CarbonylDB; P38960; -.
DR iPTMnet; P38960; -.
DR MaxQB; P38960; -.
DR PaxDb; P38960; -.
DR PRIDE; P38960; -.
DR EnsemblFungi; YDR082W_mRNA; YDR082W; YDR082W.
DR GeneID; 851655; -.
DR KEGG; sce:YDR082W; -.
DR SGD; S000002489; STN1.
DR VEuPathDB; FungiDB:YDR082W; -.
DR eggNOG; ENOG502S0RQ; Eukaryota.
DR HOGENOM; CLU_582904_0_0_1; -.
DR InParanoid; P38960; -.
DR OMA; CVAGWKW; -.
DR BioCyc; YEAST:G3O-29687-MON; -.
DR EvolutionaryTrace; P38960; -.
DR PRO; PR:P38960; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P38960; protein.
DR GO; GO:0000781; C:chromosome, telomeric region; IC:ComplexPortal.
DR GO; GO:1990879; C:CST complex; IPI:SGD.
DR GO; GO:0043047; F:single-stranded telomeric DNA binding; IDA:SGD.
DR GO; GO:0061770; F:translation elongation factor binding; IDA:SGD.
DR GO; GO:0051974; P:negative regulation of telomerase activity; IC:ComplexPortal.
DR GO; GO:0032211; P:negative regulation of telomere maintenance via telomerase; IMP:SGD.
DR GO; GO:0032210; P:regulation of telomere maintenance via telomerase; IC:ComplexPortal.
DR GO; GO:0016233; P:telomere capping; IMP:SGD.
DR Gene3D; 3.30.1370.230; -; 1.
DR InterPro; IPR024263; Stn1_C_fungi.
DR InterPro; IPR038240; Stn1_C_sf.
DR InterPro; IPR018856; Stn1_N.
DR Pfam; PF10451; Stn1; 1.
DR Pfam; PF12659; Stn1_C; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chromosome; Reference proteome; Telomere.
FT CHAIN 1..494
FT /note="Protein STN1"
FT /id="PRO_0000072279"
FT DNA_BIND 62..159
FT /note="OB"
FT REGION 311..397
FT /note="Winged helix-turn-helix (wHTH) 1"
FT REGION 396..494
FT /note="Winged helix-turn-helix (wHTH) 2"
FT MUTAGEN 397
FT /note="D->A: Modest telomere elongation phenotype."
FT /evidence="ECO:0000269|PubMed:20008938"
FT MUTAGEN 466
FT /note="W->E: Elongated telomeres."
FT /evidence="ECO:0000269|PubMed:20008938"
FT MUTAGEN 466
FT /note="W->R: Elongated telomeres and severe growth
FT defects."
FT /evidence="ECO:0000269|PubMed:20008938"
FT HELIX 315..325
FT /evidence="ECO:0007829|PDB:3KEY"
FT STRAND 326..332
FT /evidence="ECO:0007829|PDB:3KEY"
FT HELIX 333..337
FT /evidence="ECO:0007829|PDB:3KEY"
FT HELIX 340..356
FT /evidence="ECO:0007829|PDB:3KEY"
FT HELIX 365..382
FT /evidence="ECO:0007829|PDB:3KEY"
FT STRAND 385..390
FT /evidence="ECO:0007829|PDB:3KEY"
FT TURN 391..394
FT /evidence="ECO:0007829|PDB:3KEY"
FT STRAND 395..397
FT /evidence="ECO:0007829|PDB:3KEY"
FT HELIX 399..417
FT /evidence="ECO:0007829|PDB:3KEY"
FT STRAND 421..426
FT /evidence="ECO:0007829|PDB:3KEY"
FT HELIX 427..434
FT /evidence="ECO:0007829|PDB:3KEY"
FT HELIX 441..458
FT /evidence="ECO:0007829|PDB:3KEY"
FT TURN 460..462
FT /evidence="ECO:0007829|PDB:3KEY"
FT STRAND 463..470
FT /evidence="ECO:0007829|PDB:3KEY"
FT STRAND 482..489
FT /evidence="ECO:0007829|PDB:3KEY"
FT TURN 491..493
FT /evidence="ECO:0007829|PDB:3KEY"
SQ SEQUENCE 494 AA; 57511 MW; 61B691C8D4A2E650 CRC64;
MDKYGHIAHQ EGDVCYYIPR LFKYNSYYSG TEDVRIFVGD LKYRMRVSLQ ICEKYYDRRL
SMLFWKNHPL QQIHLIGCII GLQFKWIGKQ EYIFFQLDDC TSDSSLVGYT SDMRFLTCKV
KKDSILSWGL NITDLIGLTL HVYGQASLNY QELQVEYLRL CYSLTEEIDH WKITMNMREQ
LDTPWSLSDF VIGELFTQEQ EWTPETSQIE VVNPDFVGIG YKTPESKRNE TTFIEQLQEE
RLKDELEIIS PYNSTDTSNS VHSLSFRFVS SLKDFPETHF LNSGDQIDNG NDEQLKKLEY
QSANLPVMIP NRTSAKSNLM LILLGLQMKE ISNSDLYKLK EVRSVVTSLA SFLFQQQNVG
VMKSFDSLEK EAFRDLVNRL VSQGLIGLKD KTSETFDLLP LKNLFEYAEK RISVLMKLQC
YTGTVQLSHV QEKLHLPYIT TNGIVDVFKE CLKRTKKQYP EVLKNWWIDL DPKNGMEDQN
SGILLHLEYA AAYS
