STNA_DROME
ID STNA_DROME Reviewed; 850 AA.
AC Q24211; Q7PLF2; Q9W5M8;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 15-AUG-2003, sequence version 3.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Protein stoned-A;
DE Short=Stn-A;
DE Short=StonedA;
GN Name=stnA; ORFNames=CG12500;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Oregon-R; TISSUE=CNS;
RX PubMed=8844157; DOI=10.1093/genetics/143.4.1699;
RA Andrews J., Smith M., Merakovsky J., Coulson M., Hannan F., Kelly L.E.;
RT "The stoned locus of Drosophila melanogaster produces a dicistronic
RT transcript and encodes two distinct polypeptides.";
RL Genetics 143:1699-1711(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP FUNCTION.
RX PubMed=9822725; DOI=10.1523/jneurosci.18-23-09638.1998;
RA Stimson D.T., Estes P.S., Smith M., Kelly L.E., Ramaswami M.;
RT "A product of the Drosophila stoned locus regulates neurotransmitter
RT release.";
RL J. Neurosci. 18:9638-9649(1998).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=10407025; DOI=10.1523/jneurosci.19-14-05847.1999;
RA Fergestad T., Davis W.S., Broadie K.;
RT "The stoned proteins regulate synaptic vesicle recycling in the presynaptic
RT terminal.";
RL J. Neurosci. 19:5847-5860(1999).
RN [6]
RP INTERACTION WITH SYT, AND MUTANT STN-TS2.
RX PubMed=11069931; DOI=10.1523/jneurosci.20-22-08254.2000;
RA Phillips A.M., Smith M., Ramaswami M., Kelly L.E.;
RT "The products of the Drosophila stoned locus interact with synaptic
RT vesicles via synaptotagmin.";
RL J. Neurosci. 20:8254-8261(2000).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11160392; DOI=10.1523/jneurosci.21-04-01218.2001;
RA Fergestad T., Broadie K.;
RT "Interaction of stoned and synaptotagmin in synaptic vesicle endocytosis.";
RL J. Neurosci. 21:1218-1227(2001).
RN [8]
RP FUNCTION.
RX PubMed=11312288; DOI=10.1523/jneurosci.21-09-03034.2001;
RA Stimson D.T., Estes P.S., Rao S., Krishnan K.S., Kelly L.E., Ramaswami M.;
RT "Drosophila stoned proteins regulate the rate and fidelity of synaptic
RT vesicle internalization.";
RL J. Neurosci. 21:3034-3044(2001).
CC -!- FUNCTION: Adapter protein involved in endocytic recycling of synaptic
CC vesicles membranes. May act by mediating the retrieval of synaptotagmin
CC protein Syt from the plasma membrane, thereby facilitating the
CC internalization of multiple synaptic vesicles from the plasma membrane.
CC {ECO:0000269|PubMed:10407025, ECO:0000269|PubMed:11160392,
CC ECO:0000269|PubMed:11312288, ECO:0000269|PubMed:9822725}.
CC -!- SUBUNIT: Interacts with the second C2 domain of Syt.
CC {ECO:0000269|PubMed:11069931}.
CC -!- INTERACTION:
CC Q24211; P21521: Syt1; NbExp=2; IntAct=EBI-604915, EBI-484504;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Synapse. Cytoplasmic vesicle,
CC secretory vesicle, synaptic vesicle. Note=Colocalizes with synaptic
CC vesicle pools. Colocalizes with the endocytic network within synaptic
CC boutons.
CC -!- DEVELOPMENTAL STAGE: Present at synaptic connections both in the CNS
CC and in neuromuscular junctions in the mature embryo (20-22h) and
CC throughout larval development. In the third instar larva, it is
CC expressed in all synaptic bouton types, including I, II and III
CC boutons. {ECO:0000269|PubMed:10407025}.
CC -!- DOMAIN: The Asp-Pro-Phe (DPF) motifs, which are found in many
CC presynatic proteins, are thought to mediate an interaction with AP-
CC 2alpha. {ECO:0000250}.
CC -!- MISCELLANEOUS: StnB, which is involved in the same pathway, is derived
CC from the same bicistronic transcript that encodes these two different
CC proteins.
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DR EMBL; U54982; AAC16665.1; -; mRNA.
DR EMBL; AE014298; EAA46058.2; -; Genomic_DNA.
DR PIR; T13352; T13352.
DR RefSeq; NP_001036316.1; NM_001042851.3.
DR RefSeq; NP_001036317.1; NM_001042852.3.
DR RefSeq; NP_001285537.1; NM_001298608.1.
DR RefSeq; NP_001285538.1; NM_001298609.1.
DR AlphaFoldDB; Q24211; -.
DR BioGRID; 78289; 2.
DR IntAct; Q24211; 3.
DR STRING; 7227.FBpp0290833; -.
DR PaxDb; Q24211; -.
DR EnsemblMetazoa; FBtr0301618; FBpp0290833; FBgn0016976.
DR EnsemblMetazoa; FBtr0301619; FBpp0290834; FBgn0016976.
DR EnsemblMetazoa; FBtr0344112; FBpp0310531; FBgn0016976.
DR EnsemblMetazoa; FBtr0344465; FBpp0310836; FBgn0016976.
DR GeneID; 3355164; -.
DR KEGG; dme:Dmel_CG12500; -.
DR CTD; 3355164; -.
DR FlyBase; FBgn0016976; stnA.
DR VEuPathDB; VectorBase:FBgn0016976; -.
DR eggNOG; ENOG502QUZ2; Eukaryota.
DR HOGENOM; CLU_014962_0_0_1; -.
DR InParanoid; Q24211; -.
DR OMA; MHKLAKG; -.
DR PhylomeDB; Q24211; -.
DR BioGRID-ORCS; 3355164; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 3355164; -.
DR PRO; PR:Q24211; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0016976; Expressed in brain and 4 other tissues.
DR Genevisible; Q24211; DM.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0030139; C:endocytic vesicle; IDA:UniProtKB.
DR GO; GO:0008021; C:synaptic vesicle; IGI:FlyBase.
DR GO; GO:0048488; P:synaptic vesicle endocytosis; IMP:UniProtKB.
DR GO; GO:0048489; P:synaptic vesicle transport; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoplasmic vesicle; Endocytosis; Reference proteome; Repeat;
KW Synapse.
FT CHAIN 1..850
FT /note="Protein stoned-A"
FT /id="PRO_0000072280"
FT REGION 1..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 26..290
FT /note="Interaction with Syt"
FT REGION 125..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 345..375
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 412..498
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 573..610
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 634..673
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 738..760
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 800..825
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 224..226
FT /note="DPF 1"
FT MOTIF 461..463
FT /note="DPF 2"
FT MOTIF 535..537
FT /note="DPF 3"
FT MOTIF 666..668
FT /note="DPF 4"
FT MOTIF 755..757
FT /note="DPF 5"
FT MOTIF 823..825
FT /note="DPF 6"
FT COMPBIAS 1..16
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..44
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 68..91
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 426..441
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 574..606
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 653..673
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 35
FT /note="K->M: In stn-TS2; induce behavioral defects.
FT Homozygous adults are viable but exhibit sluggish and
FT uncoordinated behavior."
FT CONFLICT 463
FT /note="F -> L (in Ref. 1; AAC16665)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 850 AA; 93109 MW; 2773741640B73757 CRC64;
MLKLPKGLKK KKKKSKKDQE LFTEEELEQY KRDLKAKQEA AATKSDAGES DGASSDVEAH
HEPVAFNSGL GSGSSSSILN AQQQLSDQNQ GAAGGDEEWA KFKALTSGVD SILHKTQDEL
DRIKKESFYQ RLPSAAEKKK QKEEEAARLE AEQQEREKQR LGQIEAQRDK LAEAVVQLSE
SEEEAGDYEA DDIFATDYIE AITSGELQLA VVPDSPVLAE DGPDPFDTAY AEKVIVGADR
AKGNKKLVSL GAAVEVLSGR VDREHAVALA NPKRKLRKGI QNLLLSESIE LADSEAELLA
ATSNAEPQHN LLDDLDEELS ESSVPIDLSV SLHLHLIKHK QPVEEEEELE QKGRENQLLN
PDLSEFDSLK DEEDDEFAEL AAESLTKKEE VTVVSQVVLP VAQLPTEAFE AGSWAEFEEQ
SGQEPGKPKR PPPPVRPPTG PHIVPGAIYV SEDEEENPED DPFNTNYAEQ VIKKTTVLEE
DDDFDPRAEE HATEPPFLAA PQRDLLAGSA TDLSQVVPAP LAPTLSVDQE AEDFDPFDTS
AVSALVQPKS TELRFLEREL LNYSGLDGVT LKHSLSDQDF DPRADQKEPA APQVKLEQKE
TDFDTAQRKS SLSLNIQAKS VGFLVPASDL LGAGNELGAS KKPLTPYYAP SDNRLQERER
EAEDVDPFDT SHVPEAKLSD IELKHIEKDL ISVPANLRHS LSDPDFDPRA PPTPVPAEVL
LAVEENINIK VLTPAQDRKK LTNSGGSGKS EEDIDPFDTS IAANLQPGQT ELKLLENELL
PETKTLVTDV LDVQSDAQEL GLGDKVLTPS THSRPSLPAQ DIDPFDTSIA ENLAPGEAEI
KLLESELIER
