STP2_ARATH
ID STP2_ARATH Reviewed; 498 AA.
AC Q9LNV3; Q9SMV5;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 3.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Sugar transport protein 2;
DE AltName: Full=Hexose transporter 2;
GN Name=STP2; OrderedLocusNames=At1g07340; ORFNames=F22G5.32;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14, FUNCTION, TISSUE SPECIFICITY,
RP BIOPHYSICOCHEMICAL PROPERTIES, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=10074716; DOI=10.1046/j.1365-313x.1999.00372.x;
RA Truernit E., Stadler R., Baier K., Sauer N.;
RT "A male gametophyte-specific monosaccharide transporter in Arabidopsis.";
RL Plant J. 17:191-201(1999).
RN [5]
RP GENE FAMILY.
RX PubMed=16923188; DOI=10.1186/1471-2148-6-64;
RA Johnson D.A., Hill J.P., Thomas M.A.;
RT "The monosaccharide transporter gene family in land plants is ancient and
RT shows differential subfamily expression and expansion across lineages.";
RL BMC Evol. Biol. 6:64-64(2006).
CC -!- FUNCTION: Mediates an active uptake of hexoses, probably by
CC sugar/hydrogen symport. Can transport glucose, 3-O-methylglucose,
CC xylose, mannose, fructose and galactose. {ECO:0000269|PubMed:10074716}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=50 uM for galactose {ECO:0000269|PubMed:10074716};
CC KM=20 uM for 3-O-methylglucose {ECO:0000269|PubMed:10074716};
CC -!- INTERACTION:
CC Q9LNV3; Q9LPQ3: MKK7; NbExp=3; IntAct=EBI-25512884, EBI-2128593;
CC Q9LNV3; Q39021: MPK1; NbExp=3; IntAct=EBI-25512884, EBI-1238932;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Pollen specific (at protein level).
CC {ECO:0000269|PubMed:10074716}.
CC -!- DEVELOPMENTAL STAGE: Specifically expressed in pollen grains when the
CC callose of microspores tetrads undergoes degradation, between early
CC tetrad and late trinucleate stages (at protein level).
CC {ECO:0000269|PubMed:10074716}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
CC transporter (TC 2.A.1.1) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79565.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF79565.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BX813610; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AC022464; AAF79565.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28111.1; -; Genomic_DNA.
DR EMBL; BX813610; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AJ001362; CAB59188.1; -; Genomic_DNA.
DR PIR; E86208; E86208.
DR RefSeq; NP_172214.5; NM_100608.5.
DR AlphaFoldDB; Q9LNV3; -.
DR SMR; Q9LNV3; -.
DR BioGRID; 22486; 2.
DR IntAct; Q9LNV3; 2.
DR STRING; 3702.AT1G07340.1; -.
DR TCDB; 2.A.1.1.48; the major facilitator superfamily (mfs).
DR PaxDb; Q9LNV3; -.
DR PRIDE; Q9LNV3; -.
DR ProteomicsDB; 228362; -.
DR EnsemblPlants; AT1G07340.1; AT1G07340.1; AT1G07340.
DR GeneID; 837245; -.
DR Gramene; AT1G07340.1; AT1G07340.1; AT1G07340.
DR KEGG; ath:AT1G07340; -.
DR Araport; AT1G07340; -.
DR TAIR; locus:2025102; AT1G07340.
DR eggNOG; KOG0254; Eukaryota.
DR HOGENOM; CLU_001265_30_5_1; -.
DR InParanoid; Q9LNV3; -.
DR OMA; DENRHFH; -.
DR OrthoDB; 430696at2759; -.
DR PhylomeDB; Q9LNV3; -.
DR SABIO-RK; Q9LNV3; -.
DR PRO; PR:Q9LNV3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LNV3; baseline and differential.
DR Genevisible; Q9LNV3; AT.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:TAIR.
DR GO; GO:0015145; F:monosaccharide transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015749; P:monosaccharide transmembrane transport; TAS:TAIR.
DR GO; GO:0009555; P:pollen development; TAS:TAIR.
DR CDD; cd17361; MFS_STP; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR044778; MFS_STP/MST-like_plant.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR045262; STP/PLT_plant.
DR InterPro; IPR003663; Sugar/inositol_transpt.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR PANTHER; PTHR23500; PTHR23500; 1.
DR Pfam; PF00083; Sugar_tr; 1.
DR PRINTS; PR00171; SUGRTRNSPORT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00879; SP; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Sugar transport; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..498
FT /note="Sugar transport protein 2"
FT /id="PRO_0000050432"
FT TOPO_DOM 1..22
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 23..43
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..401
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..441
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 450..470
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 471..498
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 498 AA; 55023 MW; CEA14ED417C93F93 CRC64;
MAVGSMNVEE GTKAFPAKLT GQVFLCCVIA AVGGLMFGYD IGISGGVTSM DTFLLDFFPH
VYEKKHRVHE NNYCKFDDQL LQLFTSSLYL AGIFASFISS YVSRAFGRKP TIMLASIFFL
VGAILNLSAQ ELGMLIGGRI LLGFGIGFGN QTVPLFISEI APARYRGGLN VMFQFLITIG
ILAASYVNYL TSTLKNGWRY SLGGAAVPAL ILLIGSFFIH ETPASLIERG KDEKGKQVLR
KIRGIEDIEL EFNEIKYATE VATKVKSPFK ELFTKSENRP PLVCGTLLQF FQQFTGINVV
MFYAPVLFQT MGSGDNASLI STVVTNGVNA IATVISLLVV DFAGRRCLLM EGALQMTATQ
MTIGGILLAH LKLVGPITGH AVPLIVLILI CVYVSGFAWS WGPLGWLVPS EIYPLEVRNA
GYFCAVAMNM VCTFIIGQFF LSALCRFRSL LFFFFGIMNI IMGLFVVFFL PETKGVPIEE
MAEKRWKTHP RWKKYFKD
