STP4_ARATH
ID STP4_ARATH Reviewed; 514 AA.
AC Q39228; Q570J6;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Sugar transport protein 4;
DE AltName: Full=Hexose transporter 4;
GN Name=STP4; OrderedLocusNames=At3g19930; ORFNames=MPN9.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=8989877; DOI=10.2307/3870459;
RA Truernit E., Schmid J., Epple P., Illig J., Sauer N.;
RT "The sink-specific and stress-regulated Arabidopsis STP4 gene: enhanced
RT expression of a gene encoding a monosaccharide transporter by wounding,
RT elicitors, and pathogen challenge.";
RL Plant Cell 8:2169-2182(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 430-514.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INDUCTION.
RX PubMed=11161062; DOI=10.1104/pp.125.2.1074;
RA Moran P.J., Thompson G.A.;
RT "Molecular responses to aphid feeding in Arabidopsis in relation to plant
RT defense pathways.";
RL Plant Physiol. 125:1074-1085(2001).
RN [7]
RP INDUCTION, AND TISSUE SPECIFICITY.
RX PubMed=12805612; DOI=10.1104/pp.103.021428;
RA Fotopoulos V., Gilbert M.J., Pittman J.K., Marvier A.C., Buchanan A.J.,
RA Sauer N., Hall J.L., Williams L.E.;
RT "The monosaccharide transporter gene, AtSTP4, and the cell-wall invertase,
RT Atbetafruct1, are induced in Arabidopsis during infection with the fungal
RT biotroph Erysiphe cichoracearum.";
RL Plant Physiol. 132:821-829(2003).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=15060130; DOI=10.1074/mcp.m400001-mcp200;
RA Marmagne A., Rouet M.-A., Ferro M., Rolland N., Alcon C., Joyard J.,
RA Garin J., Barbier-Brygoo H., Ephritikhine G.;
RT "Identification of new intrinsic proteins in Arabidopsis plasma membrane
RT proteome.";
RL Mol. Cell. Proteomics 3:675-691(2004).
RN [9]
RP GENE FAMILY.
RX PubMed=16923188; DOI=10.1186/1471-2148-6-64;
RA Johnson D.A., Hill J.P., Thomas M.A.;
RT "The monosaccharide transporter gene family in land plants is ancient and
RT shows differential subfamily expression and expansion across lineages.";
RL BMC Evol. Biol. 6:64-64(2006).
CC -!- FUNCTION: Mediates an active uptake of hexoses, probably by
CC sugar/hydrogen symport. Can transport glucose, methylglucose,
CC galactose, xylose and mannose, but not fructose.
CC {ECO:0000269|PubMed:8989877}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=15 uM for glucose;
CC KM=100 uM for 3-O-methylglucose;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15060130};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Mostly in flowers and roots, especially in anthers,
CC including pollen, and root tips. Also present in some hydathodes.
CC {ECO:0000269|PubMed:12805612, ECO:0000269|PubMed:8989877}.
CC -!- INDUCTION: Induced locally by wounding, elicitors such as chitin,
CC bacterial pathogens such as P.syringae, compatible fungal pathogens
CC such as A.brassicicola, E.cichoracearum and F.oxysporum, and
CC incompatible fungal pathogens such as B.graminis. Also induced by aphid
CC feeding. {ECO:0000269|PubMed:11161062, ECO:0000269|PubMed:12805612,
CC ECO:0000269|PubMed:8989877}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
CC transporter (TC 2.A.1.1) family. {ECO:0000305}.
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DR EMBL; X66857; CAA47325.1; -; mRNA.
DR EMBL; AB025631; BAB01308.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76309.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64813.1; -; Genomic_DNA.
DR EMBL; AF367352; AAK32938.1; -; mRNA.
DR EMBL; AF428342; AAL16272.1; -; mRNA.
DR EMBL; AY133592; AAM91422.1; -; mRNA.
DR EMBL; AK220712; BAD93826.1; -; mRNA.
DR PIR; S25009; S25009.
DR RefSeq; NP_001326818.1; NM_001338428.1.
DR RefSeq; NP_188627.1; NM_112883.5.
DR AlphaFoldDB; Q39228; -.
DR SMR; Q39228; -.
DR BioGRID; 6863; 24.
DR IntAct; Q39228; 18.
DR STRING; 3702.AT3G19930.1; -.
DR TCDB; 2.A.1.1.49; the major facilitator superfamily (mfs).
DR PaxDb; Q39228; -.
DR PRIDE; Q39228; -.
DR ProteomicsDB; 245221; -.
DR EnsemblPlants; AT3G19930.1; AT3G19930.1; AT3G19930.
DR EnsemblPlants; AT3G19930.2; AT3G19930.2; AT3G19930.
DR GeneID; 821531; -.
DR Gramene; AT3G19930.1; AT3G19930.1; AT3G19930.
DR Gramene; AT3G19930.2; AT3G19930.2; AT3G19930.
DR KEGG; ath:AT3G19930; -.
DR Araport; AT3G19930; -.
DR TAIR; locus:2092286; AT3G19930.
DR eggNOG; KOG0254; Eukaryota.
DR HOGENOM; CLU_001265_30_5_1; -.
DR InParanoid; Q39228; -.
DR OMA; IVARTIC; -.
DR OrthoDB; 430696at2759; -.
DR PhylomeDB; Q39228; -.
DR PRO; PR:Q39228; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q39228; baseline and differential.
DR Genevisible; Q39228; AT.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015145; F:monosaccharide transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0008506; F:sucrose:proton symporter activity; IDA:TAIR.
DR GO; GO:0015770; P:sucrose transport; TAS:TAIR.
DR CDD; cd17361; MFS_STP; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR044778; MFS_STP/MST-like_plant.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR045262; STP/PLT_plant.
DR InterPro; IPR003663; Sugar/inositol_transpt.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR PANTHER; PTHR23500; PTHR23500; 1.
DR Pfam; PF00083; Sugar_tr; 1.
DR PRINTS; PR00171; SUGRTRNSPORT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00879; SP; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Sugar transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..514
FT /note="Sugar transport protein 4"
FT /id="PRO_0000050434"
FT TOPO_DOM 1..22
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 23..43
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 426..446
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 451..471
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 472..514
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 514 AA; 57095 MW; 33C6104CA2E455B1 CRC64;
MAGGFVSQTP GVRNYNYKLT PKVFVTCFIG AFGGLIFGYD LGISGGVTSM EPFLEEFFPY
VYKKMKSAHE NEYCRFDSQL LTLFTSSLYV AALVSSLFAS TITRVFGRKW SMFLGGFTFF
IGSAFNGFAQ NIAMLLIGRI LLGFGVGFAN QSVPVYLSEM APPNLRGAFN NGFQVAIIFG
IVVATIINYF TAQMKGNIGW RISLGLACVP AVMIMIGALI LPDTPNSLIE RGYTEEAKEM
LQSIRGTNEV DEEFQDLIDA SEESKQVKHP WKNIMLPRYR PQLIMTCFIP FFQQLTGINV
ITFYAPVLFQ TLGFGSKASL LSAMVTGIIE LLCTFVSVFT VDRFGRRILF LQGGIQMLVS
QIAIGAMIGV KFGVAGTGNI GKSDANLIVA LICIYVAGFA WSWGPLGWLV PSEISPLEIR
SAAQAINVSV NMFFTFLVAQ LFLTMLCHMK FGLFFFFAFF VVIMTIFIYL MLPETKNVPI
EEMNRVWKAH WFWGKFIPDE AVNMGAAEMQ QKSV
