STP9_ARATH
ID STP9_ARATH Reviewed; 517 AA.
AC Q9SX48; O81706;
DT 24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Sugar transport protein 9;
DE AltName: Full=Hexose transporter 9;
GN Name=STP9; OrderedLocusNames=At1g50310; ORFNames=F14I3.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, ACTIVITY
RP REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE SPECIFICITY.
RC STRAIN=cv. C24; TISSUE=Flower;
RX PubMed=12970485; DOI=10.1104/pp.103.026674;
RA Schneidereit A., Scholz-Starke J., Buettner M.;
RT "Functional characterization and expression analyses of the glucose-
RT specific AtSTP9 monosaccharide transporter in pollen of Arabidopsis.";
RL Plant Physiol. 133:182-190(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 408-475.
RC STRAIN=cv. C24;
RA Baier K., Truernit E., Sauer N.;
RL Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=16923188; DOI=10.1186/1471-2148-6-64;
RA Johnson D.A., Hill J.P., Thomas M.A.;
RT "The monosaccharide transporter gene family in land plants is ancient and
RT shows differential subfamily expression and expansion across lineages.";
RL BMC Evol. Biol. 6:64-64(2006).
CC -!- FUNCTION: Mediates an active uptake of hexoses, probably by
CC sugar/hydrogen symport. Mostly transports glucose, and barely
CC galactose, xylose and mannose. {ECO:0000269|PubMed:12970485}.
CC -!- ACTIVITY REGULATION: Inhibited by uncouplers such as 2,4-dinitrophenol
CC and carbonyl cyanide-m-chlorophenyl-hydrazone.
CC {ECO:0000269|PubMed:12970485}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=84 uM for glucose (at pH 5.5) {ECO:0000269|PubMed:12970485};
CC pH dependence:
CC Optimum pH is 5.5. {ECO:0000269|PubMed:12970485};
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Pollen specific (at protein level).
CC {ECO:0000269|PubMed:12970485}.
CC -!- DEVELOPMENTAL STAGE: Transcripts accumulate in mature pollen before and
CC during germination, but translation starts only when pollen germination
CC initiates, and continues in pollen tubes.
CC {ECO:0000269|PubMed:12970485}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
CC transporter (TC 2.A.1.1) family. {ECO:0000305}.
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DR EMBL; AJ344336; CAC69072.1; -; mRNA.
DR EMBL; AC007980; AAD50040.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32535.1; -; Genomic_DNA.
DR EMBL; AJ001662; CAA04907.1; -; Genomic_DNA.
DR PIR; D96539; D96539.
DR RefSeq; NP_175449.1; NM_103915.2.
DR AlphaFoldDB; Q9SX48; -.
DR SMR; Q9SX48; -.
DR BioGRID; 26678; 1.
DR STRING; 3702.AT1G50310.1; -.
DR PaxDb; Q9SX48; -.
DR PRIDE; Q9SX48; -.
DR ProteomicsDB; 245349; -.
DR EnsemblPlants; AT1G50310.1; AT1G50310.1; AT1G50310.
DR GeneID; 841453; -.
DR Gramene; AT1G50310.1; AT1G50310.1; AT1G50310.
DR KEGG; ath:AT1G50310; -.
DR Araport; AT1G50310; -.
DR TAIR; locus:2011957; AT1G50310.
DR eggNOG; KOG0254; Eukaryota.
DR HOGENOM; CLU_001265_30_5_1; -.
DR InParanoid; Q9SX48; -.
DR OMA; MFQAKFD; -.
DR OrthoDB; 430696at2759; -.
DR PhylomeDB; Q9SX48; -.
DR PRO; PR:Q9SX48; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SX48; baseline and differential.
DR Genevisible; Q9SX48; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015145; F:monosaccharide transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR CDD; cd17361; MFS_STP; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR044778; MFS_STP/MST-like_plant.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR045262; STP/PLT_plant.
DR InterPro; IPR003663; Sugar/inositol_transpt.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR PANTHER; PTHR23500; PTHR23500; 1.
DR Pfam; PF00083; Sugar_tr; 1.
DR PRINTS; PR00171; SUGRTRNSPORT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00879; SP; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Sugar transport; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..517
FT /note="Sugar transport protein 9"
FT /id="PRO_0000050439"
FT TOPO_DOM 1..18
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 19..39
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..312
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 324..344
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 429..449
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 454..474
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 475..517
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 471
FT /note="I -> T (in Ref. 4; CAA04907)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 517 AA; 56280 MW; 44A9D5EBAAA6FA57 CRC64;
MAGGAFVSEG GGGGNSYEGG VTVFVIMTCI VAAMGGLLFG YDLGISGGVT SMEEFLSKFF
PEVDKQMHEA RRETAYCKFD NQLLQLFTSS LYLAALASSF VASAVTRKYG RKISMFVGGV
AFLIGSLFNA FATNVAMLIV GRLLLGVGVG FANQSTPVYL SEMAPAKIRG ALNIGFQMAI
TIGILIANLI NYGTSQMAKN GWRVSLGLAA VPAVIMVIGS FVLPDTPNSM LERGKYEQAR
EMLQKIRGAD NVDEEFQDLC DACEAAKKVD NPWKNIFQQA KYRPALVFCS AIPFFQQITG
INVIMFYAPV LFKTLGFADD ASLISAVITG AVNVVSTLVS IYAVDRYGRR ILFLEGGIQM
IVSQIVVGTL IGMKFGTTGS GTLTPATADW ILAFICLYVA GFAWSWGPLG WLVPSEICPL
EIRPAGQAIN VSVNMFFTFL IGQFFLTMLC HMKFGLFYFF GGMVAVMTVF IYFLLPETKG
VPIEEMGRVW KQHPFWKRYM PDDAVIGGGE ENYVKEV
