ID   STRA_STRGR              Reviewed;         307 AA.
AC   P08077;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Streptomycin 6-kinase;
DE            EC=2.7.1.72;
DE   AltName: Full=APH(6);
DE   AltName: Full=Streptidine kinase;
DE   AltName: Full=Streptomycin 6-phosphotransferase;
GN   Name=aphD; Synonyms=sph, strA;
OS   Streptomyces griseus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=N2-3-11;
RX   PubMed=3118332; DOI=10.1093/nar/15.19.8041;
RA   Distler J., Ebert A., Mansouri K., Pissowotzki K., Stockmann M.,
RA   Piepersberg W.;
RT   "Gene cluster for streptomycin biosynthesis in Streptomyces griseus:
RT   nucleotide sequence of three genes and analysis of transcriptional
RT   activity.";
RL   Nucleic Acids Res. 15:8041-8056(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 23345 / DSM 40236 / JCM 4644 / NBRC 12875 / NCIMB 13023 / NRRL
RC   B-2682 / VKM Ac-800 / IMRU 3463;
RX   PubMed=3029728; DOI=10.1093/nar/15.4.1819;
RA   Tohyama H., Okami Y., Umezawa H.;
RT   "Nucleotide sequence of the streptomycinphosphotransferase and
RT   amidinotransferase genes from Streptomyces griseus.";
RL   Nucleic Acids Res. 15:1819-1833(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3039306; DOI=10.1007/bf00330443;
RA   Distler J., Braun C., Ebert A., Piepersberg W.;
RT   "Gene cluster for streptomycin biosynthesis in Streptomyces griseus:
RT   analysis of a central region including the major resistance gene.";
RL   Mol. Gen. Genet. 208:204-210(1987).
CC   -!- FUNCTION: The aminoglycoside phosphotransferases achieve inactivation
CC       of their antibiotic substrates by phosphorylation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + streptomycin = ADP + H(+) + streptomycin 6-phosphate;
CC         Xref=Rhea:RHEA:22268, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57787, ChEBI:CHEBI:58007, ChEBI:CHEBI:456216;
CC         EC=2.7.1.72;
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; Y00459; CAA68516.1; -; Genomic_DNA.
DR   EMBL; X05045; CAA28717.1; -; Genomic_DNA.
DR   EMBL; X05647; CAA29134.1; -; Genomic_DNA.
DR   PIR; A26674; A26674.
DR   PIR; S06355; KISM6G.
DR   RefSeq; WP_063842181.1; NG_047458.1.
DR   AlphaFoldDB; P08077; -.
DR   SMR; P08077; -.
DR   BioCyc; MetaCyc:MON-14016; -.
DR   GO; GO:0050300; F:aminoglycoside 6-kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0019748; P:secondary metabolic process; IEA:InterPro.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR006748; NH2Glyco/OHUrea_AB-resist_kin.
DR   Pfam; PF04655; APH_6_hur; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Kinase; Nucleotide-binding;
KW   Transferase.
FT   CHAIN           1..307
FT                   /note="Streptomycin 6-kinase"
FT                   /id="PRO_0000204815"
FT   ACT_SITE        201
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         133..145
FT                   /ligand="streptomycin"
FT                   /ligand_id="ChEBI:CHEBI:58007"
FT                   /evidence="ECO:0000250"
FT   VARIANT         34..39
FT                   /note="ALVARC -> GSRRAI (in strain: ISP 5236)"
FT   VARIANT         115..120
FT                   /note="DGSRTL -> GRFADV (in strain: ISP 5236)"
FT   VARIANT         181..188
FT                   /note="GWASAVAE -> AVGVGREP (in strain: ISP 5236)"
FT   VARIANT         225
FT                   /note="G -> A (in strain: ISP 5236)"
FT   VARIANT         262
FT                   /note="S -> A (in strain: ISP 5236)"
SQ   SEQUENCE   307 AA;  33244 MW;  382DFD60DF4028AA CRC64;
     MSSSDHIHVP DGLAESYSRS GGEEGRAWIA GLPALVARCV DRWELKRDGG VRSGEASLVV
     PVLRADGTRA ALKLQMPREE TTAALIGLRA WGGDGMVRLL DHDEESSTML LERLDGSRTL
     ASVEDDDEAM GVLAGLLNRL HSVPAPPGLR GLGEIAGAMV EEVPSAVDSL ADPEDRSRLR
     GWASAVAELV GEPGDRVLHW DLHYENVLAA EREPWLAIDP EPLVGDPGFD LWPALDTGWE
     RIEATGDARR VVRRRFDLLT ESLELDRGRA AGWTLARLLQ NTLWDIEDGL TAIAPSQIAV
     AEALAKP