ID   STRB1_STRGA             Reviewed;         348 AA.
AC   Q54258;
DT   11-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Inosamine-phosphate amidinotransferase 1;
DE            EC=2.1.4.2;
DE   AltName: Full=Aminocyclitol amidinotransferase;
DE            Short=ADT;
DE   AltName: Full=Inosamine-phosphate amidinotransferase I;
GN   Name=strB1;
OS   Streptomyces glaucescens.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1907;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 40716 / ETH 22794 / Tue 49;
RA   Mayer G., Piepersberg W.;
RL   Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes two non-consecutive transamidination reactions. It
CC       converts scyllo-inosamine 4-phosphate into N-amidino-scyllo-inosamine
CC       4-phosphate and N1-amidinostreptamine 6-phosphate into streptidine 6-
CC       phosphate (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-amino-1-deoxy-scyllo-inositol 4-phosphate + L-arginine = 1-
CC         guanidino-1-deoxy-scyllo-inositol 4-phosphate + L-ornithine;
CC         Xref=Rhea:RHEA:13265, ChEBI:CHEBI:32682, ChEBI:CHEBI:46911,
CC         ChEBI:CHEBI:57656, ChEBI:CHEBI:58325; EC=2.1.4.2;
CC   -!- PATHWAY: Antibiotic biosynthesis; streptomycin biosynthesis.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the amidinotransferase family. {ECO:0000305}.
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DR   EMBL; X78974; CAA55571.1; -; Genomic_DNA.
DR   EMBL; AJ006985; CAA07380.1; -; Genomic_DNA.
DR   PIR; S44229; S44229.
DR   RefSeq; WP_043497526.1; NZ_CP009438.1.
DR   AlphaFoldDB; Q54258; -.
DR   SMR; Q54258; -.
DR   STRING; 1907.SGLAU_01160; -.
DR   eggNOG; COG1834; Bacteria.
DR   OMA; GGFHCAT; -.
DR   OrthoDB; 592329at2; -.
DR   BRENDA; 2.1.4.2; 6022.
DR   UniPathway; UPA00066; -.
DR   GO; GO:0015069; F:scyllo-inosamine-4-phosphate amidinotransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019872; P:streptomycin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR033195; AmidinoTrfase.
DR   PANTHER; PTHR10488; PTHR10488; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Streptomycin biosynthesis; Transferase.
FT   CHAIN           1..348
FT                   /note="Inosamine-phosphate amidinotransferase 1"
FT                   /id="PRO_0000215476"
FT   ACT_SITE        179
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        227
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        332
FT                   /note="Amidino-cysteine intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   348 AA;  38592 MW;  F457819EDE103FA0 CRC64;
     MSLVSVHNEW DPLEEIIVGT AVGARVPRAD RSVFAVEYAD EYDSQDQVPA GPYPDRVLKE
     TEEELHVLSE ELTKLGVTVR RPGQRDNSAL VATPDWQTDG FHDYCPRDGL LAVGQTVIES
     PMALRARFLE SLAYKDILLE YFASGARWLS APKPRLADEM YEPTAPAGQR LTDLEPVFDA
     ANVLRFGTDL LYLVSDSGNE LGAKWLQSAL GSTYKVHPCR GLYASTHVDS TIVPLRPGLV
     LVNPARVNDD NMPDFLRSWQ TVVCPELVDI GFTGDKPHCS VWIGMNLLVV RPDLAVVDRR
     QTGLIKVLEK HGVDVLPLQL THSRTLGGGF HCATLDVRRT GSLETYRF