ID   STRB2_STRGR             Reviewed;         349 AA.
AC   P29780;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Putative inosamine-phosphate amidinotransferase 2;
DE            EC=2.1.4.2;
DE   AltName: Full=Aminocyclitol amidinotransferase;
DE            Short=ADT;
DE   AltName: Full=Inosamine-phosphate amidinotransferase II;
GN   Name=strB2;
OS   Streptomyces griseus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=N2-3-11;
RX   PubMed=1661369; DOI=10.1007/bf00293829;
RA   Pissowotzki K., Mansouri K., Piepersberg W.;
RT   "Genetics of streptomycin production in Streptomyces griseus: molecular
RT   structure and putative function of genes strELMB2N.";
RL   Mol. Gen. Genet. 231:113-123(1991).
CC   -!- FUNCTION: It is not obvious if strB2 participates in streptomycin
CC       biosynthesis as an inosamine-phosphate amidinotransferase. Attempt to
CC       measure its activity have failed and the nucleophilic cysteine which is
CC       the key residue for amidine transfer is not conserved but replaced by a
CC       glycine residue.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-amino-1-deoxy-scyllo-inositol 4-phosphate + L-arginine = 1-
CC         guanidino-1-deoxy-scyllo-inositol 4-phosphate + L-ornithine;
CC         Xref=Rhea:RHEA:13265, ChEBI:CHEBI:32682, ChEBI:CHEBI:46911,
CC         ChEBI:CHEBI:57656, ChEBI:CHEBI:58325; EC=2.1.4.2;
CC   -!- PATHWAY: Antibiotic biosynthesis; streptomycin biosynthesis.
CC   -!- SIMILARITY: Belongs to the amidinotransferase family. {ECO:0000305}.
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DR   EMBL; X62567; CAA44441.1; -; Genomic_DNA.
DR   PIR; S18620; XJSMIG.
DR   AlphaFoldDB; P29780; -.
DR   SMR; P29780; -.
DR   BRENDA; 2.1.4.2; 6037.
DR   UniPathway; UPA00066; -.
DR   GO; GO:0015069; F:scyllo-inosamine-4-phosphate amidinotransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019872; P:streptomycin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR033195; AmidinoTrfase.
DR   PANTHER; PTHR10488; PTHR10488; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Streptomycin biosynthesis; Transferase.
FT   CHAIN           1..349
FT                   /note="Putative inosamine-phosphate amidinotransferase 2"
FT                   /id="PRO_0000215477"
FT   SITE            333
FT                   /note="Corresponds to active site cysteine in strB1"
SQ   SEQUENCE   349 AA;  38219 MW;  6C49FA018C3ABAA7 CRC64;
     MSLVGVHNEW DPLEEVVVGT ARRPCAGPDR SLLAVEYAEY AGPGRWQEVP TGPYPDRALK
     ETEDELEELC EELRGLGVTV RRPGARDSAA PVRTPDWESD GYGDLCPRDG LLVVGDTVIE
     APMALRARFL ESLAYKELLV EYLAAGSRWI SAPKPRLAEG MYSPSGPSGE RLRDLEPVFD
     AANVLRLGTD LLYLVSDSGN ELGARWLQSA LGAAYTVHPC RGLYSSTHID STLVPLRPGL
     VLVNPARVTD DNLPGVLRTW QRIECPALAG LGYAGDVPHC STWIGMSLLV VRPGLVVVDS
     RHRELMRVLE RHGVDVLPLK LTHARMLGGG FHGVTLDIRR TGALETYRF