STRH_STRPN
ID STRH_STRPN Reviewed; 1312 AA.
AC P49610;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2001, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Beta-N-acetylhexosaminidase;
DE EC=3.2.1.52;
DE Flags: Precursor;
GN Name=strH; OrderedLocusNames=SP_0057;
OS Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=170187;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 12213;
RX PubMed=7721787; DOI=10.1074/jbc.270.15.8805;
RA Clarke V.A., Platt N., Butters T.D.;
RT "Cloning and expression of the beta-N-acetylglucosaminidase gene from
RT Streptococcus pneumoniae. Generation of truncated enzymes with modified
RT aglycon specificity.";
RL J. Biol. Chem. 270:8805-8814(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-334 / TIGR4;
RX PubMed=11463916; DOI=10.1126/science.1061217;
RA Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT "Complete genome sequence of a virulent isolate of Streptococcus
RT pneumoniae.";
RL Science 293:498-506(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine
CC residues in N-acetyl-beta-D-hexosaminides.; EC=3.2.1.52;
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-
CC ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00477}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 20 family. {ECO:0000305}.
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DR EMBL; L36923; AAC41450.1; -; Genomic_DNA.
DR EMBL; AE005672; AAK74246.1; -; Genomic_DNA.
DR PIR; A56390; A56390.
DR PIR; E95006; E95006.
DR RefSeq; WP_000679952.1; NZ_AKVY01000001.1.
DR PDB; 2LTJ; NMR; -; A=1050-1140.
DR PDB; 2YL5; X-ray; 2.15 A; A/B/C/D=627-1064.
DR PDB; 2YL6; X-ray; 1.60 A; A=181-614.
DR PDB; 2YL8; X-ray; 1.75 A; A=181-614.
DR PDB; 2YL9; X-ray; 2.65 A; A/B/C/D=627-1062.
DR PDB; 2YLA; X-ray; 2.70 A; A/B/C/D=627-1064.
DR PDB; 2YLL; X-ray; 1.85 A; A=181-614.
DR PDB; 4AZ5; X-ray; 1.73 A; A=181-614.
DR PDB; 4AZ6; X-ray; 1.36 A; A=181-613.
DR PDB; 4AZ7; X-ray; 1.70 A; A=181-613.
DR PDB; 4AZB; X-ray; 2.10 A; A=181-614.
DR PDB; 4AZC; X-ray; 2.09 A; A/B/C/D=627-1064.
DR PDB; 4AZG; X-ray; 2.40 A; A/B=627-1064.
DR PDB; 4AZH; X-ray; 2.22 A; A/B/C/D=627-1064.
DR PDB; 4AZI; X-ray; 1.98 A; A/B=627-1064.
DR PDBsum; 2LTJ; -.
DR PDBsum; 2YL5; -.
DR PDBsum; 2YL6; -.
DR PDBsum; 2YL8; -.
DR PDBsum; 2YL9; -.
DR PDBsum; 2YLA; -.
DR PDBsum; 2YLL; -.
DR PDBsum; 4AZ5; -.
DR PDBsum; 4AZ6; -.
DR PDBsum; 4AZ7; -.
DR PDBsum; 4AZB; -.
DR PDBsum; 4AZC; -.
DR PDBsum; 4AZG; -.
DR PDBsum; 4AZH; -.
DR PDBsum; 4AZI; -.
DR AlphaFoldDB; P49610; -.
DR SMR; P49610; -.
DR STRING; 170187.SP_0057; -.
DR CAZy; GH20; Glycoside Hydrolase Family 20.
DR PRIDE; P49610; -.
DR EnsemblBacteria; AAK74246; AAK74246; SP_0057.
DR KEGG; spn:SP_0057; -.
DR eggNOG; COG3064; Bacteria.
DR eggNOG; COG3525; Bacteria.
DR eggNOG; COG3583; Bacteria.
DR OMA; QPMAFND; -.
DR BioCyc; SPNE170187:G1FZB-64-MON; -.
DR Proteomes; UP000000585; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0004563; F:beta-N-acetylhexosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102148; F:N-acetyl-beta-D-galactosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR011098; G5_dom.
DR InterPro; IPR015883; Glyco_hydro_20_cat.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR019931; LPXTG_anchor.
DR InterPro; IPR005877; YSIRK_signal_dom.
DR Pfam; PF07501; G5; 2.
DR Pfam; PF00728; Glyco_hydro_20; 2.
DR Pfam; PF00746; Gram_pos_anchor; 1.
DR Pfam; PF04650; YSIRK_signal; 1.
DR SMART; SM01208; G5; 2.
DR SUPFAM; SSF51445; SSF51445; 2.
DR TIGRFAMs; TIGR01168; YSIRK_signal; 1.
DR PROSITE; PS51109; G5; 2.
DR PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell wall; Glycosidase; Hydrolase; Peptidoglycan-anchor;
KW Repeat; Secreted; Signal.
FT SIGNAL 1..33
FT /evidence="ECO:0000255"
FT CHAIN 34..1284
FT /note="Beta-N-acetylhexosaminidase"
FT /id="PRO_0000012020"
FT PROPEP 1285..1312
FT /note="Removed by sortase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT /id="PRO_0000012021"
FT DOMAIN 1059..1138
FT /note="G5 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT DOMAIN 1150..1230
FT /note="G5 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT REGION 38..178
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 176..616
FT /note="Catalytic domain 1"
FT REGION 621..1046
FT /note="Catalytic domain 2"
FT REGION 1244..1290
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 1281..1285
FT /note="LPXTG sorting signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT COMPBIAS 38..65
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..104
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 105..120
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 121..178
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1284
FT /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT CONFLICT 39
FT /note="Missing (in Ref. 1; AAC41450)"
FT /evidence="ECO:0000305"
FT CONFLICT 69
FT /note="V -> E (in Ref. 1; AAC41450)"
FT /evidence="ECO:0000305"
FT CONFLICT 169
FT /note="A -> E (in Ref. 1; AAC41450)"
FT /evidence="ECO:0000305"
FT CONFLICT 617
FT /note="Q -> L (in Ref. 1; AAC41450)"
FT /evidence="ECO:0000305"
FT CONFLICT 1045
FT /note="V -> A (in Ref. 1; AAC41450)"
FT /evidence="ECO:0000305"
FT CONFLICT 1161
FT /note="E -> K (in Ref. 1; AAC41450)"
FT /evidence="ECO:0000305"
FT CONFLICT 1171
FT /note="C -> R (in Ref. 1; AAC41450)"
FT /evidence="ECO:0000305"
FT CONFLICT 1267
FT /note="V -> A (in Ref. 1; AAC41450)"
FT /evidence="ECO:0000305"
FT HELIX 181..184
FT /evidence="ECO:0007829|PDB:4AZ6"
FT STRAND 186..193
FT /evidence="ECO:0007829|PDB:4AZ6"
FT TURN 194..196
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 201..214
FT /evidence="ECO:0007829|PDB:4AZ6"
FT STRAND 218..228
FT /evidence="ECO:0007829|PDB:4AZ6"
FT STRAND 237..239
FT /evidence="ECO:0007829|PDB:4AZ6"
FT STRAND 242..244
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 246..260
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 271..283
FT /evidence="ECO:0007829|PDB:4AZ6"
FT STRAND 287..297
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 299..308
FT /evidence="ECO:0007829|PDB:4AZ6"
FT STRAND 314..317
FT /evidence="ECO:0007829|PDB:4AZ6"
FT STRAND 320..326
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 331..348
FT /evidence="ECO:0007829|PDB:4AZ6"
FT TURN 349..351
FT /evidence="ECO:0007829|PDB:4AZ6"
FT STRAND 353..358
FT /evidence="ECO:0007829|PDB:4AZ6"
FT TURN 362..368
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 371..380
FT /evidence="ECO:0007829|PDB:4AZ6"
FT STRAND 383..385
FT /evidence="ECO:0007829|PDB:4AZB"
FT HELIX 390..407
FT /evidence="ECO:0007829|PDB:4AZ6"
FT STRAND 411..416
FT /evidence="ECO:0007829|PDB:4AZ6"
FT TURN 417..420
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 421..423
FT /evidence="ECO:0007829|PDB:4AZ6"
FT STRAND 435..438
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 451..456
FT /evidence="ECO:0007829|PDB:4AZ6"
FT STRAND 461..463
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 466..468
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 484..493
FT /evidence="ECO:0007829|PDB:4AZ6"
FT STRAND 510..517
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 527..540
FT /evidence="ECO:0007829|PDB:4AZ6"
FT TURN 541..544
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 550..557
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 568..582
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 590..592
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 593..608
FT /evidence="ECO:0007829|PDB:4AZ6"
FT HELIX 627..630
FT /evidence="ECO:0007829|PDB:4AZI"
FT STRAND 631..638
FT /evidence="ECO:0007829|PDB:4AZI"
FT TURN 639..641
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 646..659
FT /evidence="ECO:0007829|PDB:4AZI"
FT STRAND 663..673
FT /evidence="ECO:0007829|PDB:4AZI"
FT STRAND 682..684
FT /evidence="ECO:0007829|PDB:4AZI"
FT STRAND 687..689
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 691..705
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 716..728
FT /evidence="ECO:0007829|PDB:4AZI"
FT STRAND 732..742
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 744..752
FT /evidence="ECO:0007829|PDB:4AZI"
FT STRAND 759..770
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 775..792
FT /evidence="ECO:0007829|PDB:4AZI"
FT TURN 793..795
FT /evidence="ECO:0007829|PDB:4AZC"
FT STRAND 796..802
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 808..810
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 815..821
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 825..841
FT /evidence="ECO:0007829|PDB:4AZI"
FT STRAND 845..850
FT /evidence="ECO:0007829|PDB:4AZI"
FT TURN 851..854
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 855..857
FT /evidence="ECO:0007829|PDB:4AZI"
FT STRAND 868..871
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 884..889
FT /evidence="ECO:0007829|PDB:4AZI"
FT STRAND 893..896
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 899..901
FT /evidence="ECO:0007829|PDB:4AZI"
FT STRAND 902..904
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 909..911
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 916..925
FT /evidence="ECO:0007829|PDB:4AZI"
FT TURN 936..938
FT /evidence="ECO:0007829|PDB:4AZI"
FT STRAND 943..951
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 961..974
FT /evidence="ECO:0007829|PDB:4AZI"
FT TURN 975..978
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 984..990
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 1002..1013
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 1021..1023
FT /evidence="ECO:0007829|PDB:4AZI"
FT HELIX 1024..1036
FT /evidence="ECO:0007829|PDB:4AZI"
FT STRAND 1055..1058
FT /evidence="ECO:0007829|PDB:2LTJ"
FT STRAND 1060..1063
FT /evidence="ECO:0007829|PDB:2LTJ"
FT STRAND 1065..1073
FT /evidence="ECO:0007829|PDB:2LTJ"
FT STRAND 1085..1088
FT /evidence="ECO:0007829|PDB:2LTJ"
FT STRAND 1092..1094
FT /evidence="ECO:0007829|PDB:2LTJ"
FT STRAND 1099..1109
FT /evidence="ECO:0007829|PDB:2LTJ"
FT STRAND 1118..1120
FT /evidence="ECO:0007829|PDB:2LTJ"
FT STRAND 1131..1134
FT /evidence="ECO:0007829|PDB:2LTJ"
SQ SEQUENCE 1312 AA; 144550 MW; 503375B5257A90B5 CRC64;
MKHEKQQRFS IRKYAVGAAS VLIGFAFQAQ TVAADGVTPT TTENQPTIHT VSDSPQSSEN
RTEETPKAVL QPEAPKTVET ETPATDKVAS LPKTEEKPQE EVSSTPSDKA EVVTPTSAEK
ETANKKAEEA SPKKEEAKEV DSKESNTDKT DKDKPAKKDE AKAEADKPAT EAGKERAATV
NEKLAKKKIV SIDAGRKYFS PEQLKEIIDK AKHYGYTDLH LLVGNDGLRF MLDDMSITAN
GKTYASDDVK RAIEKGTNDY YNDPNGNHLT ESQMTDLINY AKDKGIGLIP TVNSPGHMDA
ILNAMKELGI QNPNFSYFGK KSARTVDLDN EQAVAFTKAL IDKYAAYFAK KTEIFNIGLD
EYANDATDAK GWSVLQADKY YPNEGYPVKG YEKFIAYAND LARIVKSHGL KPMAFNDGIY
YNSDTSFGSF DKDIIVSMWT GGWGGYDVAS SKLLAEKGHQ ILNTNDAWYY VLGRNADGQG
WYNLDQGLNG IKNTPITSVP KTEGADIPII GGMVAAWADT PSARYSPSRL FKLMRHFANA
NAEYFAADYE SAEQALNEVP KDLNRYTAES VTAVKEAEKA IRSLDSNLSR AQQDTIDQAI
AKLQETVNNL TLTPEAQKEE EAKREVEKLA KNKVISIDAG RKYFTLNQLK RIVDKASELG
YSDVHLLLGN DGLRFLLDDM TITANGKTYA SDDVKKAIIE GTKAYYDDPN GTALTQAEVT
ELIEYAKSKD IGLIPAINSP GHMDAMLVAM EKLGIKNPQA HFDKVSKTTM DLKNEEAMNF
VKALIGKYMD FFAGKTKIFN FGTDEYANDA TSAQGWYYLK WYQLYGKFAE YANTLAAMAK
ERGLQPMAFN DGFYYEDKDD VQFDKDVLIS YWSKGWWGYN LASPQYLASK GYKFLNTNGD
WYYILGQKPE DGGGFLKKAI ENTGKTPFNQ LASTKYPEVD LPTVGSMLSI WADRPSAEYK
EEEIFELMTA FADHNKDYFR ANYNALREEL AKIPTNLEGY SKESLEALDA AKTALNYNLN
RNKQAELDTL VANLKAALQG LKPAVTHSGS LDENEVAANV ETRPELITRT EEIPFEVIKK
ENPNLPAGQE NIITAGVKGE RTHYISVLTE NGKTTETVLD SQVTKEVINQ VVEVGAPVTH
KGDESGLAPT TEVKPRLDIQ EEEIPFTTVT CENPLLLKGK TQVITKGVNG HRSNFYSVST
SADGKEVKTL VNSVVAQEAV TQIVEVGTMV THVGDENGQA AIAEEKPKLE IPSQPAPSTA
PAEESKVLPQ DPAPVVTEKK LPETGTHDSA GLVVAGLMST LAAYGLTKRK ED
