ID   STRP_STRP1              Reviewed;         440 AA.
AC   P10520; Q48WH3;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 2.
DT   25-MAY-2022, entry version 140.
DE   RecName: Full=Streptokinase A;
DE   Flags: Precursor;
GN   Name=ska; OrderedLocusNames=SPy_1979, M5005_Spy1684;
OS   Streptococcus pyogenes serotype M1.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=301447;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SF130/13 / Serotype M1;
RX   PubMed=2646590; DOI=10.1093/nar/17.3.1261;
RA   Walter F., Siegel M., Malke H.;
RT   "Nucleotide sequence of the streptokinase gene from a Streptococcus
RT   pyogenes type 1 strain.";
RL   Nucleic Acids Res. 17:1261-1261(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700294 / SF370 / Serotype M1;
RX   PubMed=11296296; DOI=10.1073/pnas.071559398;
RA   Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K.,
RA   Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P.,
RA   Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L., White J., Yuan X.,
RA   Clifton S.W., Roe B.A., McLaughlin R.E.;
RT   "Complete genome sequence of an M1 strain of Streptococcus pyogenes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-947 / MGAS5005 / Serotype M1;
RX   PubMed=16088826; DOI=10.1086/432514;
RA   Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K.,
RA   Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P.,
RA   Musser J.M.;
RT   "Evolutionary origin and emergence of a highly successful clone of serotype
RT   M1 group A Streptococcus involved multiple horizontal gene transfer
RT   events.";
RL   J. Infect. Dis. 192:771-782(2005).
CC   -!- FUNCTION: This protein is not a protease, but it activates plasminogen
CC       by complexing with it. As a potential virulence factor, it is thought
CC       to prevent the formation of effective fibrin barriers around the site
CC       of infection, thereby contributing to the invasiveness of the cells.
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DR   EMBL; X13399; CAA31765.1; -; Genomic_DNA.
DR   EMBL; AE004092; AAK34665.1; -; Genomic_DNA.
DR   EMBL; CP000017; AAZ52302.1; -; Genomic_DNA.
DR   PIR; S02724; S02724.
DR   RefSeq; NP_269944.1; NC_002737.2.
DR   AlphaFoldDB; P10520; -.
DR   SMR; P10520; -.
DR   BindingDB; P10520; -.
DR   ChEMBL; CHEMBL1293228; -.
DR   DrugCentral; P10520; -.
DR   PaxDb; P10520; -.
DR   EnsemblBacteria; AAK34665; AAK34665; SPy_1979.
DR   KEGG; spy:SPy_1979; -.
DR   KEGG; spz:M5005_Spy1684; -.
DR   PATRIC; fig|160490.10.peg.1720; -.
DR   HOGENOM; CLU_622418_0_0_9; -.
DR   OMA; HDDTNRI; -.
DR   PRO; PR:P10520; -.
DR   Proteomes; UP000000750; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0031639; P:plasminogen activation; IEA:InterPro.
DR   InterPro; IPR036120; SAK/SK_sf.
DR   InterPro; IPR008124; SK.
DR   PRINTS; PR01753; STREPKINASE.
DR   SUPFAM; SSF54328; SSF54328; 3.
PE   4: Predicted;
KW   Plasminogen activation; Reference proteome; Signal; Virulence.
FT   SIGNAL          1..26
FT   CHAIN           27..440
FT                   /note="Streptokinase A"
FT                   /id="PRO_0000022429"
FT   CONFLICT        163
FT                   /note="L -> V (in Ref. 1; CAA31765)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        345
FT                   /note="R -> G (in Ref. 1; CAA31765)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        373
FT                   /note="D -> N (in Ref. 1; CAA31765)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        428
FT                   /note="D -> Y (in Ref. 1; CAA31765)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        438
FT                   /note="K -> N (in Ref. 1; CAA31765)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   440 AA;  49924 MW;  D6227EF040B758DB CRC64;
     MKNYLSIGVI ALLFALTFGT VKSVQAIAGY GWLPDRPPIN NSQLVVSMAG IVEGTDKKVF
     INFFEIDLTS QPAHGGKTEQ GLSPKSKPFA TDNGAMPHKL EKADLLKAIQ KQLIANVHSN
     DGYFEVIDFA SDATITDRNG KVYFADKDGS VTLPTQPVQE FLLKGHVRVR PYKEKPVQNQ
     AKSVDVEYTV QFTPLNPDDD FRPGLKDTKL LKTLAIGDTI TSQELLAQAQ SILNKTHPGY
     TIYERDSSIV THDNDIFRTI LPMDQEFTYH VKNREQAYEI NPKTGIKEKT NNTDLVSEKY
     YVLKQGEKPY DPFDRSHLKL FTIKYVDVNT NELLKSEQLL TASERNLDFR DLYDPRDKAK
     LLYNNLDAFD IMDYTLTGKV EDNHDKNNRV VTVYMGKRPK GAKGSYHLAY DKDLYTEEER
     KAYSYLRDTG TPIPDNPKDK