ID   STS25_POSPM             Reviewed;         332 AA.
AC   A0A348B793;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   07-NOV-2018, sequence version 1.
DT   03-AUG-2022, entry version 13.
DE   RecName: Full=Monoterpene synthase 25 {ECO:0000303|PubMed:30105900};
DE            EC=4.2.3.- {ECO:0000269|PubMed:30105900};
DE   AltName: Full=Terpene cyclase 25 {ECO:0000303|PubMed:30105900};
GN   Name=STS-25 {ECO:0000303|PubMed:30105900};
OS   Postia placenta (strain ATCC 44394 / Madison 698-R) (Brown rot fungus)
OS   (Poria monticola).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Polyporales; Dacryobolaceae; Postia.
OX   NCBI_TaxID=561896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DOMAIN, AND CATALYTIC ACTIVITY.
RC   STRAIN=ATCC 44394 / Madison 698-R;
RX   PubMed=30105900; DOI=10.1111/1751-7915.13304;
RA   Ichinose H., Kitaoka T.;
RT   "Insight into metabolic diversity of the brown-rot basidiomycete Postia
RT   placenta responsible for sesquiterpene biosynthesis: semi-comprehensive
RT   screening of cytochrome P450 monooxygenase involved in protoilludene
RT   metabolism.";
RL   Microb. Biotechnol. 11:952-965(2018).
CC   -!- FUNCTION: Terpene cyclase that catalyzes the cyclization of geranyl
CC       diphosphate (GPP) to myrcene and linalool.
CC       {ECO:0000269|PubMed:30105900}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:I3ZNU9};
CC   -!- DOMAIN: The conserved DDXXXXD and NSE/DTE motifs are important for the
CC       catalytic activity, presumably through binding to Mg(2+).
CC       {ECO:0000305|PubMed:30105900}.
CC   -!- SIMILARITY: Belongs to the trichodiene synthase family. {ECO:0000305}.
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DR   EMBL; LC378439; BBD74531.1; -; mRNA.
DR   AlphaFoldDB; A0A348B793; -.
DR   SMR; A0A348B793; -.
DR   GO; GO:0016838; F:carbon-oxygen lyase activity, acting on phosphates; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR024652; Trichodiene_synth.
DR   Pfam; PF06330; TRI5; 1.
DR   SFLD; SFLDG01021; Trichodiene_Synthase_Like; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   1: Evidence at protein level;
KW   Lyase; Magnesium; Metal-binding.
FT   CHAIN           1..332
FT                   /note="Monoterpene synthase 25"
FT                   /id="PRO_0000451375"
FT   MOTIF           115..121
FT                   /note="DDXXXXD motif"
FT                   /evidence="ECO:0000305|PubMed:30105900"
FT   MOTIF           240..248
FT                   /note="NSE/DTE motif"
FT                   /evidence="ECO:0000305|PubMed:30105900"
FT   BINDING         115
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P13513"
FT   BINDING         180
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P13513"
FT   BINDING         240
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P13513"
FT   BINDING         244
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P13513"
FT   BINDING         248
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P13513"
SQ   SEQUENCE   332 AA;  37340 MW;  C0ED64BDA6307BB4 CRC64;
     MSPTSDFVNT TSTAKGHEII AGLTAKPVEG STITLCKDII REFFDKVQVP SPNFTRDPEL
     EARVADIVRT WGNEEHLRPY VVTSLILTVT AYSHIANFET RVQITLFTII IIAMDDPVVF
     DSLATREFHQ RMCTGVIQDE AGMLGAFTKI LESMWDHYSG FSANTIYASA LRFVNASIIE
     NETDVTTLRS HALPFVEYKR SMTATTEAYA CFIWDKARFP DVKVYMQAIP DAMLYVSYVN
     DILSFYKEEL AGETANYIHE RAYVTGKSIP DTLRNLINET ASAVERVRDI LGEGEARAAF
     ENFAAGYIRV HTGNPRYHLK DVIGGDYIID RV