STS3_POSPM
ID STS3_POSPM Reviewed; 365 AA.
AC A0A348B781; B8PAF1;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 07-NOV-2018, sequence version 1.
DT 03-AUG-2022, entry version 18.
DE RecName: Full=Sesquiterpene synthase 3 {ECO:0000303|PubMed:30105900};
DE EC=4.2.3.- {ECO:0000269|PubMed:30105900};
DE AltName: Full=Terpene cyclase 3 {ECO:0000303|PubMed:30105900};
GN Name=STS-03 {ECO:0000303|PubMed:30105900}; ORFNames=POSPLDRAFT_99496;
OS Postia placenta (strain ATCC 44394 / Madison 698-R) (Brown rot fungus)
OS (Poria monticola).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Polyporales; Dacryobolaceae; Postia.
OX NCBI_TaxID=561896;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DOMAIN, AND CATALYTIC ACTIVITY.
RC STRAIN=ATCC 44394 / Madison 698-R;
RX PubMed=30105900; DOI=10.1111/1751-7915.13304;
RA Ichinose H., Kitaoka T.;
RT "Insight into metabolic diversity of the brown-rot basidiomycete Postia
RT placenta responsible for sesquiterpene biosynthesis: semi-comprehensive
RT screening of cytochrome P450 monooxygenase involved in protoilludene
RT metabolism.";
RL Microb. Biotechnol. 11:952-965(2018).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 44394 / Madison 698-R;
RX PubMed=19193860; DOI=10.1073/pnas.0809575106;
RA Martinez D., Challacombe J., Morgenstern I., Hibbett D., Schmoll M.,
RA Kubicek C.P., Ferreira P., Ruiz-Duenas F.J., Martinez A.T., Kersten P.,
RA Hammel K.E., Vanden Wymelenberg A., Gaskell J., Lindquist E., Sabat G.,
RA Splinter BonDurant S., Larrondo L.F., Canessa P., Vicuna R., Yadav J.,
RA Doddapaneni H., Subramanian V., Pisabarro A.G., Lavin J.L., Oguiza J.A.,
RA Master E., Henrissat B., Coutinho P.M., Harris P., Magnuson J.K.,
RA Baker S.E., Bruno K., Kenealy W., Hoegger P.J., Kuees U., Ramaiya P.,
RA Lucas S., Salamov A., Shapiro H., Tu H., Chee C.L., Misra M., Xie G.,
RA Teter S., Yaver D., James T., Mokrejs M., Pospisek M., Grigoriev I.V.,
RA Brettin T., Rokhsar D., Berka R., Cullen D.;
RT "Genome, transcriptome, and secretome analysis of wood decay fungus Postia
RT placenta supports unique mechanisms of lignocellulose conversion.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:1954-1959(2009).
CC -!- FUNCTION: Terpene cyclase that catalyzes the cyclization of farnesyl
CC diphosphate (FPP) to various sesquiterpenes, including beta-elemene
CC gamma-cadinene, delta-cadinene, and alpha-cadinene.
CC {ECO:0000269|PubMed:30105900}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate = delta-cadinene + diphosphate;
CC Xref=Rhea:RHEA:56556, ChEBI:CHEBI:33019, ChEBI:CHEBI:140564,
CC ChEBI:CHEBI:175763; Evidence={ECO:0000269|PubMed:30105900};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:56557;
CC Evidence={ECO:0000269|PubMed:30105900};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:30105900};
CC -!- DOMAIN: The conserved DDXXD and NSE/DTE motifs are important for the
CC catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000305|PubMed:30105900}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EED82142.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; LC378427; BBD74519.1; -; mRNA.
DR EMBL; EQ966293; EED82142.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; A0A348B781; -.
DR SMR; A0A348B781; -.
DR EnsemblFungi; EED82142; EED82142; POSPLDRAFT_99496.
DR KEGG; ppl:POSPLDRAFT_99496; -.
DR HOGENOM; CLU_042538_2_1_1; -.
DR Proteomes; UP000001743; Unassembled WGS sequence.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034686; Terpene_cyclase-like_2.
DR SFLD; SFLDG01020; Terpene_Cyclase_Like_2; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Lyase; Magnesium; Metal-binding; Reference proteome.
FT CHAIN 1..365
FT /note="Sesquiterpene synthase 3"
FT /id="PRO_0000451388"
FT MOTIF 117..121
FT /note="DDXXD motif"
FT /evidence="ECO:0000305|PubMed:30105900"
FT MOTIF 253..261
FT /note="NSE/DTE motif"
FT /evidence="ECO:0000305|PubMed:30105900"
FT BINDING 117
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q9UR08"
FT BINDING 117
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q9UR08"
FT BINDING 207
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9UR08"
FT BINDING 253
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q9UR08"
FT BINDING 257
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q9UR08"
FT BINDING 261
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250|UniProtKB:Q9UR08"
FT BINDING 357..358
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9UR08"
FT SITE 114
FT /note="Plays a critical role in the stabilization of
FT intermediate cation"
FT /evidence="ECO:0000250|UniProtKB:B5HDJ6"
SQ SEQUENCE 365 AA; 40862 MW; 4C25185BA8EDB65C CRC64;
MGSISSTPSQ KSPVFPARSL LPSDIVAVRP EGDEAKVLKF PDLVKSIPFP LRLNPYIRFV
SAESDAFIIE YANFSEKQRN RFIGLNAGLL CGMCYAECGP EQLRVCCDFM SFLFNLDDWS
DEFDTAGTKG LEEAVMNTLY HPDTYVSDTV AARTARSWWT RMLKTVGPRC RQRFVETLGF
YFKAILQQAA DRSSKTIPDL ETYISLRRDT SGCKTGFALI EYAAGIDLPN EVVDHPIIQS
LLDATNDCVS WANDILSYNR EQSRGDTHNL VPVIMQTVGI DRQAAIDYAG DLCNKSVAHF
LEGKAALPSW GKEVDVQVEQ YVQGLEDWII ANAEWSFMTE RYFGKDGPKI RKGLQVSLLP
VVGFD
