ID   STSY_RAUMA              Reviewed;         342 AA.
AC   P68174; P15324;
DT   25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Strictosidine synthase;
DE            EC=4.3.3.2;
DE   Flags: Precursor; Fragment;
GN   Name=STR1;
OS   Rauvolfia mannii.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Rauvolfiinae; Rauvolfia.
OX   NCBI_TaxID=4062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1567228; DOI=10.1016/0003-9861(92)90746-j;
RA   Bracher D., Kutchan T.M.;
RT   "Strictosidine synthase from Rauvolfia serpentina: analysis of a gene
RT   involved in indole alkaloid biosynthesis.";
RL   Arch. Biochem. Biophys. 294:717-723(1992).
CC   -!- FUNCTION: Catalyzes the stereospecific condensation of tryptamine with
CC       secologanin to form strictosidine, the key intermediate of indole
CC       alkaloid biosynthesis.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3alpha(S)-strictosidine + H2O = secologanin + tryptamine;
CC         Xref=Rhea:RHEA:15013, ChEBI:CHEBI:15377, ChEBI:CHEBI:18002,
CC         ChEBI:CHEBI:57887, ChEBI:CHEBI:58193; EC=4.3.3.2;
CC   -!- PATHWAY: Alkaloid biosynthesis; 3alpha(S)-strictosidine biosynthesis;
CC       3alpha(S)-strictosidine from secologanin and tryptamine: step 1/1.
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Vacuole.
CC   -!- SIMILARITY: Belongs to the strictosidine synthase family.
CC       {ECO:0000305}.
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DR   EMBL; X63431; CAA45025.1; -; Genomic_DNA.
DR   PIR; S29894; S29894.
DR   AlphaFoldDB; P68174; -.
DR   SMR; P68174; -.
DR   PRIDE; P68174; -.
DR   UniPathway; UPA00311; UER00447.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   GO; GO:0016844; F:strictosidine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   Gene3D; 2.120.10.30; -; 1.
DR   InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR   InterPro; IPR018119; Strictosidine_synth_cons-reg.
DR   InterPro; IPR004141; Strictosidine_synthase.
DR   PANTHER; PTHR10426:SF57; PTHR10426:SF57; 1.
DR   Pfam; PF03088; Str_synth; 1.
PE   3: Inferred from homology;
KW   Alkaloid metabolism; Glycoprotein; Lyase; Signal; Vacuole.
FT   SIGNAL          <1..20
FT                   /evidence="ECO:0000250"
FT   CHAIN           21..342
FT                   /note="Strictosidine synthase"
FT                   /id="PRO_0000033333"
FT   CARBOHYD        89
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   NON_TER         1
SQ   SEQUENCE   342 AA;  37960 MW;  61F4B51826E269E8 CRC64;
     KLSDSQTMAL FTVFLLFLSS SLALSSPILK EILIEAPSYA PNSFTFDSTN KGFYTSVQDG
     RVIKYEGPNS GFVDFAYASP YWNKAFCENS TDAEKRPLCG RTYDISYNLQ NNQLYIVDCY
     YHLSVVGSEG GHATQLATSV DGVPFKWLYA VTVDQRTGIV YFTDVSTLYD DRGVQQIMDT
     SDKTGRLIKY DPSTKETTLL LKELHVPGGA EVSADSSFVL VAEFLSHQIV KYWLEGPKKG
     TAEVLVKIPN PGNIKRNADG HFWVSSSEEL DGNMHGRVDP KGIKFDEFGN ILEVIPLPPP
     FAGEHFEQIQ EHDGLLYIGT LFHGSVGILV YDKKGNSFVS SH