STTB_ASPTN
ID STTB_ASPTN Reviewed; 530 AA.
AC Q0CRW7;
DT 12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT 12-AUG-2020, sequence version 2.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Cytochrome P450 monooxygenase sttB {ECO:0000303|PubMed:28604695};
DE EC=1.-.-.- {ECO:0000305|PubMed:28604695};
DE AltName: Full=Aspterpenacid biosynthesis cluster protein sttB {ECO:0000303|PubMed:34085529};
DE AltName: Full=Ophiobolin family sesterterpenoid biosynthesis cluster protein B {ECO:0000303|PubMed:28604695};
GN Name=sttB {ECO:0000303|PubMed:28604695}; ORFNames=ATEG_03567;
OS Aspergillus terreus (strain NIH 2624 / FGSC A1156).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=341663;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIH 2624 / FGSC A1156;
RA Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M.,
RA Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K.,
RA Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W.,
RA Nierman W.C., Milne T., Madden K.;
RT "Annotation of the Aspergillus terreus NIH2624 genome.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX PubMed=28604695; DOI=10.1038/nchembio.2408;
RA Clevenger K.D., Bok J.W., Ye R., Miley G.P., Verdan M.H., Velk T., Chen C.,
RA Yang K., Robey M.T., Gao P., Lamprecht M., Thomas P.M., Islam M.N.,
RA Palmer J.M., Wu C.C., Keller N.P., Kelleher N.L.;
RT "A scalable platform to identify fungal secondary metabolites and their
RT gene clusters.";
RL Nat. Chem. Biol. 13:895-901(2017).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=34085529; DOI=10.1021/acs.orglett.1c01361;
RA Jiang L., Zhang X., Sato Y., Zhu G., Minami A., Zhang W., Ozaki T., Zhu B.,
RA Wang Z., Wang X., Lv K., Zhang J., Wang Y., Gao S., Liu C., Hsiang T.,
RA Zhang L., Oikawa H., Liu X.;
RT "Genome-based discovery of enantiomeric pentacyclic sesterterpenes
RT catalyzed by fungal bifunctional terpene synthases.";
RL Org. Lett. 23:4645-4650(2021).
CC -!- FUNCTION: Cytochrome P450 monooxygenase; part of the gene cluster that
CC mediates the biosynthesis of aspterpenacids (PubMed:28604695,
CC PubMed:34085529). Performs the C22-oxidative modification of the
CC terpene synthase sttA product preaspterpenacid I to produce
CC preaspterpenacid II (PubMed:34085529). It has still to be determined
CC how preaspterpenacid II is further modified to produce aspterpenacids
CC (Probable). {ECO:0000269|PubMed:28604695, ECO:0000269|PubMed:34085529,
CC ECO:0000305|PubMed:34085529}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P04798};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000269|PubMed:28604695, ECO:0000269|PubMed:34085529}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Abolishes the production of the ophiobolin family
CC sesterterpenoid. {ECO:0000269|PubMed:28604695}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAU36841.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; CH476597; EAU36841.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_001212745.1; XM_001212745.1.
DR AlphaFoldDB; Q0CRW7; -.
DR SMR; Q0CRW7; -.
DR EnsemblFungi; EAU36841; EAU36841; ATEG_03567.
DR GeneID; 4317925; -.
DR eggNOG; KOG0157; Eukaryota.
DR HOGENOM; CLU_022195_1_0_1; -.
DR OrthoDB; 614788at2759; -.
DR UniPathway; UPA00213; -.
DR Proteomes; UP000007963; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00465; EP450IV.
DR SUPFAM; SSF48264; SSF48264; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..530
FT /note="Cytochrome P450 monooxygenase sttB"
FT /id="PRO_0000450609"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 5
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 230
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 530 AA; 60100 MW; 244F7BBC03D71B77 CRC64;
MSISNMTSTV WPDLQVPAIE HNHLPILTVA LLTGIASAVY INVSSVQDPC KVGSIPTVKR
PRLLDAYRSG VWWRIFVPRL VPYIEEGYHK YNKNDQPFRI WLGGFQAYAY VLPERYLDKI
KNMPESEASF AAMANKYFHT GLPTGEVNNL VLQVASKLVN GNLATIKTLM QGEVQKALAR
EIGSPRQWTK INAWQVARKT TEAPGLRVVF GEELANDKTF VTGVSEFVSN ITVYAFTLRY
INLGPLRDFI LYLVHWRHRR SLPAVLTPLN NVITERKKVR SNRHISDDEE SFDCIQWALD
QPVSDDCKTA EAIARRLVVI SLGTIDTVAG VLVKQLTHLA SHPECHEEIR AEIRECLAED
DNGWTLKSTG RMKKLESFIQ ESLRMSSGAI SLSGMRIVTG SGFRLDDNTV LPRDSFIAIP
TRNILYDPEV FPEPEKFDPF RFYKIKEDEK NAGSRSNRRD IRASWLAFGY GRQACPGRFY
AINAMKTILG EILLKYDIRL AEKQAPRIDI DLDPMLAPVR STDLEFRVRA
