STUM_DROME
ID STUM_DROME Reviewed; 1870 AA.
AC Q9W2E1; Q7YU83;
DT 11-JUN-2014, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 3.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Protein stum;
GN Name=stum; ORFNames=CG30263;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S.;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND TISSUE
RP SPECIFICITY.
RX PubMed=24626929; DOI=10.1126/science.1247761;
RA Desai B.S., Chadha A., Cook B.;
RT "The stum gene is essential for mechanical sensing in proprioceptive
RT neurons.";
RL Science 343:1256-1259(2014).
CC -!- FUNCTION: Required for locomotion and mechanical sensing in
CC proprioceptive neurons by transducing dendrite stretching into cellular
CC responses. Required for transduction of mechanical stimuli in a
CC specific subpopulation of proprioceptive neurons that sense joint
CC angles. Probably does not constitute the mechanically activated channel
CC and may rather serve as an accessory module required for the proper
CC localization or function of the transduction channels.
CC {ECO:0000269|PubMed:24626929}.
CC -!- SUBCELLULAR LOCATION: Cell projection, dendrite
CC {ECO:0000269|PubMed:24626929}. Membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}. Note=Specifically localizes to the
CC distal part of the sensory dendrite. Does not accumulate in any other
CC part of the cell.
CC -!- TISSUE SPECIFICITY: Expressed in limited populations of proprioceptive
CC neurons that sense a mechanical property of specific joints. In the
CC legs, localizes to 3 labeled neurons: one at the femur-tibia joint, the
CC second at the tibia-tarsus joint, and the third spanning the second
CC tarsal segment. Dendrites of stum-expressing neurons in legs are
CC stretched by both flexion and extension of corresponding joints.
CC {ECO:0000269|PubMed:24626929}.
CC -!- DISRUPTION PHENOTYPE: Impaired walking characterized by walking
CC uncoordination and reduced climbing speed. Type I neurons are
CC apparently unaffected. Abolition of responses to stretching: although
CC the dendritic stretching is not significantly affected, the Ca(2+)
CC responses to both acute and obtuse joint angles are abolished in the
CC mutant. Axons and cell bodies are indistinguishable from controls,
CC while the sensory dendrite exhibit abnormalities: in some of the stum-
CC expressing neurons the tip of the dendrite is overgrown and extended
CC into the distal segment of the joint. Expression of the mouse ortholog
CC (AC Q0VBF8) in mutant flies rescues the phenotype.
CC {ECO:0000269|PubMed:24626929}.
CC -!- MISCELLANEOUS: Was named stumble because of the walking impairment
CC phenotype. {ECO:0000305|PubMed:24626929}.
CC -!- SIMILARITY: Belongs to the SPEC3 family. Stum subfamily. {ECO:0000305}.
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DR EMBL; AE013599; AAF46751.3; -; Genomic_DNA.
DR EMBL; BT009937; AAQ22406.1; -; mRNA.
DR RefSeq; NP_001261125.2; NM_001274196.2.
DR RefSeq; NP_611603.3; NM_137759.5.
DR AlphaFoldDB; Q9W2E1; -.
DR SMR; Q9W2E1; -.
DR BioGRID; 63099; 14.
DR IntAct; Q9W2E1; 10.
DR STRING; 7227.FBpp0304307; -.
DR PaxDb; Q9W2E1; -.
DR EnsemblMetazoa; FBtr0273308; FBpp0271816; FBgn0050263.
DR EnsemblMetazoa; FBtr0347184; FBpp0312495; FBgn0050263.
DR GeneID; 37473; -.
DR KEGG; dme:Dmel_CG30263; -.
DR UCSC; CG30263-RB; d. melanogaster.
DR CTD; 375057; -.
DR FlyBase; FBgn0050263; stum.
DR VEuPathDB; VectorBase:FBgn0050263; -.
DR eggNOG; ENOG502S270; Eukaryota.
DR GeneTree; ENSGT00390000008003; -.
DR HOGENOM; CLU_242905_0_0_1; -.
DR InParanoid; Q9W2E1; -.
DR OMA; PSVQFNM; -.
DR OrthoDB; 41496at2759; -.
DR PhylomeDB; Q9W2E1; -.
DR SignaLink; Q9W2E1; -.
DR BioGRID-ORCS; 37473; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37473; -.
DR PRO; PR:Q9W2E1; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0050263; Expressed in second segment of antenna (Drosophila) and 1 other tissue.
DR ExpressionAtlas; Q9W2E1; differential.
DR Genevisible; Q9W2E1; DM.
DR GO; GO:0030425; C:dendrite; IDA:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0071683; C:sensory dendrite; IDA:UniProtKB.
DR GO; GO:0019230; P:proprioception; IMP:UniProtKB.
DR GO; GO:0050954; P:sensory perception of mechanical stimulus; IMP:FlyBase.
DR GO; GO:0042330; P:taxis; IMP:UniProtKB.
DR InterPro; IPR026673; SPEC3/Stum.
DR PANTHER; PTHR21676; PTHR21676; 1.
DR Pfam; PF15795; Spec3; 1.
PE 2: Evidence at transcript level;
KW Cell projection; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..1870
FT /note="Protein stum"
FT /id="PRO_0000429475"
FT TRANSMEM 1745..1765
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1766..1786
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1798..1818
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 90..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 238..266
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 323..410
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 444..535
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 606..709
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 724..1208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1261..1301
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1336..1480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1643..1663
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 13..36
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 37..51
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..107
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 323..337
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 389..410
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 444..482
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 507..533
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 650..709
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 724..759
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 782..820
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 849..881
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 911..935
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 936..991
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1031..1052
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1114..1129
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1131..1147
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1148..1197
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1261..1281
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1349..1384
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1464..1478
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 16
FT /note="P -> S (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 31
FT /note="N -> I (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 34
FT /note="L -> P (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 90
FT /note="A -> T (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 99
FT /note="P -> A (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 169
FT /note="V -> IF (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 248..249
FT /note="PT -> TA (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 396
FT /note="D -> Y (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 650
FT /note="L -> LT (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 806
FT /note="A -> G (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 874
FT /note="M -> L (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 950
FT /note="R -> P (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 1009
FT /note="V -> F (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 1445
FT /note="T -> P (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 1555
FT /note="I -> V (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
FT CONFLICT 1588..1590
FT /note="NQA -> KQE (in Ref. 3; AAQ22406)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1870 AA; 203163 MW; 33FEFA3F1C163B03 CRC64;
MQQTLSYSSS SPSPSPMPMP SASAAQTPPP NPNLTPNLNI RPSTNPIPRP RSTGGESAFQ
PAIIPVDYSY RTHTTRSILS AQSMAPSTFA SNQSFVRQPS ESDAQPYSSL FGDPVPPPLQ
PDDYFGTAAF ETLRNGSVEL PSSEEDALVA AMALNALIPA ARERIFPSVP YSRTPRGSLL
PYLQPPDIQI LPNSSGGSSS EQLGSPNYRL LAPDDILNKP DVYDLENEVE EYTVVGQRMS
FDDEEDEPTR TPSQAPAKLS VSSITTEDHR GLLSDSYSGR DWFRPAPQHF PEFTRIPRLR
PSSKMIMSMK QDEGPLSPLA TMIQDMNSSS SGGEERVAPN QSRPVSRDVV LSPKQYLEEQ
LGLERGSQRQ SLEDYRMPPP PADPPPFRRT DLTEQDDSRS VEPEHSLKRE MSPVIIRDSI
PMGGDYRVEQ PRPHKKTAFT ILSTGQAASS APRPPQQAPT RGRHASIQST STTTSSPAGR
PQISPRRRSI FGSEPRLPEL PSALGYTSHL VHQTTDDSPR PSVQFNMSGR HSRSKLAIPQ
KGILQQRDSL TSSIDTFTPR AQIRRGSIGR DPIRSLPYGQ KIKSMETLPL INDQHRSSIP
RLHYGSTMDL SRPEPAVFPP GALPRPLKPN LNPTRRQRGL LKIINKEESL THNPPRSNWC
SLDDLSRPSF DGTPNQARRR SSSTSPERRS STQTVSDRRS SNLSSVTATT SDFSFRRPTL
STLPAAQTLS RMRRKSVQAL SPNRIPNRIP SFGQRRQSIY QRDQEPKPLS RHGKIAKPST
LSPIIGTPNK DSSSAQSPNH RSSLMADAVD TPSEVSTRRD SVSRIPVRKS PDSRSSSPPK
DFGIPASGRS SRASITRSPS RTMNAGSRSP SRSMHPSRTS SRESGRPGDS RSASRGPPSR
PGSRLDQIGS RMDRPNSRLE RSSSRAERSS SRADRPSSRF DVSNSRGNSR ANSRLSINSS
SLRSSSISPA TMARNRSMRA TTPSRRKSIS LSPASAMMGR RKSISPDAVG QNRRVSSRGK
PEPPEILSRR NSRSRIPTRS TKVASKSPPS SSKKRSKSPE KPKAMGSTPK SNKVGKGVTA
KGSPKAKPKI AASAKPKTKT ESLMKPPVKN ANKTPVNGRP TTNAKKPASK AISTKDAKVE
KGKETGKSVA SKSNGKETES SVKSSKSQVT KTKPPGGTTS EAKKPATTKT HGITLEKGTN
GTDPVGGIPP LAAQVGNAVL QAVEAVPAVT SEVTVPSTPG GKGAGGANMS KLVRMSSRMS
LLSNKSTKVR SDSPQNRKVA TVQEHATEKE ETVGASSQPM SNDAAAILEK SQKTLENIQK
TVTEATDEIH KTISENLTDL KSLENDLAAD PSPTPSSGTT MARPGESASR TGTADGSIAP
QSSGELPKSP FPPTQPIEAS VSVLHPNESS AERIASVPEV ECESSDLPTA LDASESELVG
NVMPTPESGA DFKVDAKRRS PDGQGGSTAG SGGLAKSSSQ EFLEDKDNVK KGGLCGCCSK
LFMPCRRSRC SRCCRRRERN ESAEDQPVLW SGNSSATTAT NVQVIDETKP KRKKISDWLK
SSCCRSCRKK PAIEEHEAHQ EEVSKRTNQA ANMSTGPRTR GKCGLCLSKV FCCRSVNRVD
PTTGDETEMK QCCFCIPCRR GSRPNKKGST VSWRDQDPEL GIKPTESSVV EGASEAAMSA
ATDAGNDQVK EGCCKRFWLM LLCCRKRKRR ESNARRQSIR APPPSEDTRK KLHVDLVEYS
SKMKGAIPVL PLYLAWFCAF CNVVFPGLGT LLSGLFCLCV GIPRFSQFDS ARARIGSFII
NIIVAVSQFF CVLFCFVGWG WSIWWGTIML RCAKKLSKIK KVERLELEEE QRQAQLAEAG
KNGVAEADKT
