STX2_RAT
ID STX2_RAT Reviewed; 290 AA.
AC P50279; Q08846; Q08847; Q08848;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Syntaxin-2;
DE AltName: Full=Epimorphin;
GN Name=Stx2; Synonyms=Epim;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2B AND 2C).
RX PubMed=7690687; DOI=10.1016/0092-8674(93)90466-4;
RA Bennett M.K., Garcia-Arraras J.E., Elferink L.A., Peterson K.E.,
RA Fleming A.M., Hazuka C.D., Scheller R.H.;
RT "The syntaxin family of vesicular transport receptors.";
RL Cell 74:863-873(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-290.
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RX PubMed=8938452; DOI=10.1006/geno.1996.0574;
RA Zha H., Remmers E.F., Szpirer C., Szpirer J., Zhang H., Kozak C.A.,
RA Wilder R.L.;
RT "The epimorphin gene is highly conserved among humans, mice, and rats and
RT maps to human chromosome 7, mouse chromosome 5, and rat chromosome 12.";
RL Genomics 37:386-389(1996).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Essential for epithelial morphogenesis. May mediate Ca(2+)-
CC regulation of exocytosis acrosomal reaction in sperm.
CC -!- SUBUNIT: Interacts with SYT6 and SYT8; the interaction is Ca(2+)-
CC dependent.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type IV membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Comment=Additional isoforms seem to exist.;
CC Name=2A;
CC IsoId=P50279-1; Sequence=Displayed;
CC Name=2B; Synonyms=2';
CC IsoId=P50279-2; Sequence=VSP_006336;
CC Name=2C; Synonyms=2'';
CC IsoId=P50279-3; Sequence=VSP_006337;
CC -!- TISSUE SPECIFICITY: Heart, spleen, liver, and testis.
CC -!- SIMILARITY: Belongs to the syntaxin family. {ECO:0000305}.
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DR EMBL; L20823; AAA03044.1; -; mRNA.
DR EMBL; L20889; AAA03049.1; -; mRNA.
DR EMBL; L20888; AAA03048.1; -; mRNA.
DR EMBL; AH006759; AAC52908.1; -; Genomic_DNA.
DR PIR; C48213; C48213.
DR RefSeq; NP_036880.1; NM_012748.1. [P50279-1]
DR RefSeq; XP_006249312.1; XM_006249250.3. [P50279-2]
DR AlphaFoldDB; P50279; -.
DR SMR; P50279; -.
DR BioGRID; 247200; 3.
DR CORUM; P50279; -.
DR DIP; DIP-347N; -.
DR STRING; 10116.ENSRNOP00000001242; -.
DR iPTMnet; P50279; -.
DR PhosphoSitePlus; P50279; -.
DR PaxDb; P50279; -.
DR PRIDE; P50279; -.
DR Ensembl; ENSRNOT00000099417; ENSRNOP00000085955; ENSRNOG00000000936. [P50279-2]
DR GeneID; 25130; -.
DR KEGG; rno:25130; -.
DR UCSC; RGD:2558; rat. [P50279-1]
DR CTD; 2054; -.
DR RGD; 2558; Stx2.
DR eggNOG; KOG0810; Eukaryota.
DR GeneTree; ENSGT01030000234627; -.
DR HOGENOM; CLU_042423_2_2_1; -.
DR InParanoid; P50279; -.
DR OrthoDB; 1033833at2759; -.
DR PhylomeDB; P50279; -.
DR PRO; PR:P50279; -.
DR Proteomes; UP000002494; Chromosome 12.
DR Genevisible; P50279; RN.
DR GO; GO:0016323; C:basolateral plasma membrane; ISO:RGD.
DR GO; GO:0009986; C:cell surface; ISO:RGD.
DR GO; GO:0005911; C:cell-cell junction; ISO:RGD.
DR GO; GO:0031410; C:cytoplasmic vesicle; ISO:RGD.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0005615; C:extracellular space; ISO:RGD.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0030027; C:lamellipodium; ISO:RGD.
DR GO; GO:0045121; C:membrane raft; ISO:RGD.
DR GO; GO:0030496; C:midbody; IDA:RGD.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0048787; C:presynaptic active zone membrane; IBA:GO_Central.
DR GO; GO:0032991; C:protein-containing complex; ISO:RGD.
DR GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR GO; GO:0045202; C:synapse; IDA:SynGO.
DR GO; GO:0008021; C:synaptic vesicle; IBA:GO_Central.
DR GO; GO:0030133; C:transport vesicle; IDA:RGD.
DR GO; GO:0048306; F:calcium-dependent protein binding; ISO:RGD.
DR GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IMP:RGD.
DR GO; GO:0005198; F:structural molecule activity; ISO:RGD.
DR GO; GO:0007340; P:acrosome reaction; ISO:RGD.
DR GO; GO:0030154; P:cell differentiation; ISO:RGD.
DR GO; GO:0034599; P:cellular response to oxidative stress; ISO:RGD.
DR GO; GO:1903575; P:cornified envelope assembly; ISO:RGD.
DR GO; GO:0048546; P:digestive tract morphogenesis; IMP:RGD.
DR GO; GO:0030855; P:epithelial cell differentiation; IMP:RGD.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0046907; P:intracellular transport; TAS:RGD.
DR GO; GO:0030033; P:microvillus assembly; IDA:RGD.
DR GO; GO:0061952; P:midbody abscission; IMP:CACAO.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:CACAO.
DR GO; GO:0031629; P:synaptic vesicle fusion to presynaptic active zone membrane; IBA:GO_Central.
DR GO; GO:0048278; P:vesicle docking; IBA:GO_Central.
DR GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR GO; GO:0006903; P:vesicle targeting; TAS:RGD.
DR GO; GO:0016192; P:vesicle-mediated transport; TAS:RGD.
DR CDD; cd00179; SynN; 1.
DR InterPro; IPR010989; SNARE.
DR InterPro; IPR028671; STX2.
DR InterPro; IPR045242; Syntaxin.
DR InterPro; IPR006012; Syntaxin/epimorphin_CS.
DR InterPro; IPR006011; Syntaxin_N.
DR InterPro; IPR000727; T_SNARE_dom.
DR PANTHER; PTHR19957; PTHR19957; 1.
DR PANTHER; PTHR19957:SF36; PTHR19957:SF36; 1.
DR Pfam; PF05739; SNARE; 1.
DR Pfam; PF00804; Syntaxin; 1.
DR SMART; SM00503; SynN; 1.
DR SMART; SM00397; t_SNARE; 1.
DR SUPFAM; SSF47661; SSF47661; 1.
DR PROSITE; PS00914; SYNTAXIN; 1.
DR PROSITE; PS50192; T_SNARE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..290
FT /note="Syntaxin-2"
FT /id="PRO_0000210198"
FT TOPO_DOM 1..266
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..290
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT DOMAIN 193..255
FT /note="t-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
FT COILED 69..106
FT /evidence="ECO:0000255"
FT MOD_RES 14
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT VAR_SEQ 265..290
FT /note="KKWIIAAVVVAVIAVLALIIGLTVGK -> KVMFVLICVVTLLVILGIILAT
FT ALS (in isoform 2B)"
FT /evidence="ECO:0000303|PubMed:7690687"
FT /id="VSP_006336"
FT VAR_SEQ 265..290
FT /note="KKWIIAAVVVAVIAVLALIIGLTVGK -> GVLCALGRQC (in isoform
FT 2C)"
FT /evidence="ECO:0000303|PubMed:7690687"
FT /id="VSP_006337"
FT CONFLICT 146
FT /note="S -> R (in Ref. 2; AAC52908)"
FT /evidence="ECO:0000305"
FT CONFLICT 287
FT /note="T -> S (in Ref. 2; AAC52908)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 290 AA; 33359 MW; 37FBAD7ADFDA3841 CRC64;
MRDRLPDLTA CRKSDDGDNA VIITVEKDHF MDAFFHQVEE IRSSIARIAQ HVEDVKKNHS
IILSAPNPEG KIKEELEDLN KEIKKTANRI RGKLKAIEQS CDQDENGNRT SVDLRIRRTQ
HSVLSRKFVD VMTEYNEAQI LFRERSKGRI QRQLEITGRT TTDEELEEML ESGKPSIFIS
DIISDSQITR QALNEIESRH KDIMKLETSI RELHEMFMDM AMFVETQGEM VNNIERNVVN
SVDYVEHAKE ETKKAIKYQS KARRKKWIIA AVVVAVIAVL ALIIGLTVGK
