STX5_DROME
ID STX5_DROME Reviewed; 467 AA.
AC Q24509; Q7KW50; Q8T9C1;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Syntaxin-5;
DE AltName: Full=Sed5 protein;
DE AltName: Full=dSed5;
GN Name=Syx5 {ECO:0000312|EMBL:AAF53520.2}; Synonyms=Sed5; ORFNames=CG4214;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10471707; DOI=10.1093/genetics/153.1.179;
RA Ashburner M., Misra S., Roote J., Lewis S.E., Blazej R.G., Davis T.,
RA Doyle C., Galle R.F., George R.A., Harris N.L., Hartzell G., Harvey D.A.,
RA Hong L., Houston K.A., Hoskins R.A., Johnson G., Martin C., Moshrefi A.R.,
RA Palazzolo M., Reese M.G., Spradling A.C., Tsang G., Wan K.H., Whitelaw K.,
RA Celniker S.E., Rubin G.M.;
RT "An exploration of the sequence of a 2.9-Mb region of the genome of
RT Drosophila melanogaster: the Adh region.";
RL Genetics 153:179-219(1999).
RN [2] {ECO:0000312|EMBL:AAF53520.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAF53520.2}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305, ECO:0000312|EMBL:AAL39977.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537569};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305, ECO:0000312|EMBL:CAA55064.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 158-467, HOMODIMERIZATION, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=Barton {ECO:0000312|EMBL:CAA55064.1};
RC TISSUE=Embryo {ECO:0000269|PubMed:7929581};
RX PubMed=7929581; DOI=10.1083/jcb.127.2.357;
RA Banfield D.K., Lewis M.J., Rabouille C., Warren G., Pelham H.R.;
RT "Localization of Sed5, a putative vesicle targeting molecule, to the cis-
RT Golgi network involves both its transmembrane and cytoplasmic domains.";
RL J. Cell Biol. 127:357-371(1994).
CC -!- FUNCTION: Mediates endoplasmic reticulum to Golgi transport.
CC {ECO:0000250|UniProtKB:Q08851}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:7929581}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane
CC {ECO:0000269|PubMed:7929581}; Single-pass type IV membrane protein
CC {ECO:0000269|PubMed:7929581}.
CC -!- SIMILARITY: Belongs to the syntaxin family. {ECO:0000305}.
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DR EMBL; AE014134; AAF53520.2; -; Genomic_DNA.
DR EMBL; AY069832; AAL39977.1; -; mRNA.
DR EMBL; X78219; CAA55064.1; -; mRNA.
DR PIR; S43101; S43101.
DR RefSeq; NP_523582.4; NM_078858.5.
DR RefSeq; NP_599132.2; NM_134305.3.
DR AlphaFoldDB; Q24509; -.
DR SMR; Q24509; -.
DR BioGRID; 60977; 28.
DR IntAct; Q24509; 4.
DR STRING; 7227.FBpp0080389; -.
DR PaxDb; Q24509; -.
DR PRIDE; Q24509; -.
DR DNASU; 34966; -.
DR EnsemblMetazoa; FBtr0080831; FBpp0080389; FBgn0011708.
DR EnsemblMetazoa; FBtr0080832; FBpp0080390; FBgn0011708.
DR GeneID; 34966; -.
DR KEGG; dme:Dmel_CG4214; -.
DR CTD; 34966; -.
DR FlyBase; FBgn0011708; Syx5.
DR VEuPathDB; VectorBase:FBgn0011708; -.
DR eggNOG; KOG0812; Eukaryota.
DR GeneTree; ENSGT01000000214440; -.
DR HOGENOM; CLU_044998_1_1_1; -.
DR InParanoid; Q24509; -.
DR OMA; RGITGTM; -.
DR OrthoDB; 1297427at2759; -.
DR PhylomeDB; Q24509; -.
DR Reactome; R-DME-204005; COPII-mediated vesicle transport.
DR Reactome; R-DME-6807878; COPI-mediated anterograde transport.
DR Reactome; R-DME-6811438; Intra-Golgi traffic.
DR Reactome; R-DME-9013106; RHOC GTPase cycle.
DR Reactome; R-DME-9609523; Insertion of tail-anchored proteins into the endoplasmic reticulum membrane.
DR BioGRID-ORCS; 34966; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34966; -.
DR PRO; PR:Q24509; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0011708; Expressed in spermathecum and 24 other tissues.
DR ExpressionAtlas; Q24509; baseline and differential.
DR Genevisible; Q24509; DM.
DR GO; GO:0005801; C:cis-Golgi network; IDA:UniProtKB.
DR GO; GO:0012505; C:endomembrane system; HDA:FlyBase.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IDA:FlyBase.
DR GO; GO:0000139; C:Golgi membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0031201; C:SNARE complex; ISM:FlyBase.
DR GO; GO:0042803; F:protein homodimerization activity; IPI:UniProtKB.
DR GO; GO:0005484; F:SNAP receptor activity; IPI:FlyBase.
DR GO; GO:0000149; F:SNARE binding; ISM:FlyBase.
DR GO; GO:0000902; P:cell morphogenesis; IMP:FlyBase.
DR GO; GO:0006878; P:cellular copper ion homeostasis; IMP:FlyBase.
DR GO; GO:0055070; P:copper ion homeostasis; IMP:FlyBase.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006887; P:exocytosis; IMP:FlyBase.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0035167; P:larval lymph gland hemopoiesis; IMP:FlyBase.
DR GO; GO:0007112; P:male meiosis cytokinesis; IMP:FlyBase.
DR GO; GO:0009306; P:protein secretion; IMP:FlyBase.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR GO; GO:0048278; P:vesicle docking; IBA:GO_Central.
DR GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR InterPro; IPR010989; SNARE.
DR InterPro; IPR045242; Syntaxin.
DR InterPro; IPR006012; Syntaxin/epimorphin_CS.
DR InterPro; IPR000727; T_SNARE_dom.
DR PANTHER; PTHR19957; PTHR19957; 1.
DR Pfam; PF05739; SNARE; 1.
DR SMART; SM00397; t_SNARE; 1.
DR SUPFAM; SSF47661; SSF47661; 1.
DR PROSITE; PS00914; SYNTAXIN; 1.
DR PROSITE; PS50192; T_SNARE; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; ER-Golgi transport; Golgi apparatus; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..467
FT /note="Syntaxin-5"
FT /id="PRO_0000210237"
FT TOPO_DOM 1..445
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 446..466
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 467
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT DOMAIN 375..437
FT /note="t-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
FT REGION 1..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 58..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 310..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 245..269
FT /evidence="ECO:0000255"
FT COMPBIAS 12..34
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 314..335
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 467 AA; 51850 MW; DE43A6BDA649B0C4 CRC64;
MQTRRRLHQT DQQDYSSSST YTIQEDQQGG AGAGSVGTGT AGGSVGLLAQ SLVPPPTGHE
AAIHIGDNYQ SGDSISTPDY SDDKYGKAAR QWNLRAFSGH ALRTLSAAAA VVTGNQPGIN
NDSQSNYSYP ASIPTSSQFY QSKEEPQETE PEPEIFVMAA RDRTGEFANA IRSLQARNIT
RAVNIRDPRK AKQVQSYSEF MMVARFIGKN IASTYAKLEK LTMLAKKKSL FDDRPQEIQE
LTYIIKGDLN ALNQQIARLQ DISKDQRRHT NGKHLVSHSS NMVLALQSKL ASMSTDFKQI
LEVRTENLKQ QKTRRDQFSQ GPGPLAAHTV SPSTAKQGSL LLSEENQAVS IDMGSSDTTP
LLSTQTQMAI YDDSDNYVQQ RAETMQNIES TIVELGGIFQ QLAHMVKEQE EIVERIDTNV
ADAELNIEAA HGEILKYFQS VSKNRWLMIK IFGVLIFFFL FFVVFMS
