ID   STYB_PSEFL              Reviewed;         170 AA.
AC   O06835;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=NADH-dependent flavin reductase StyB;
DE            EC=1.5.1.36 {ECO:0000305|PubMed:9172343};
DE   AltName: Full=Styrene monooxygenase small component;
GN   Name=styB;
OS   Pseudomonas fluorescens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=294;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND PATHWAY.
RC   STRAIN=ST;
RX   PubMed=9172343; DOI=10.1128/aem.63.6.2232-2239.1997;
RA   Beltrametti F., Marconi A.M., Bestetti G., Colombo C., Galli E., Ruzzi M.,
RA   Zennaro E.;
RT   "Sequencing and functional analysis of styrene catabolism genes from
RT   Pseudomonas fluorescens ST.";
RL   Appl. Environ. Microbiol. 63:2232-2239(1997).
CC   -!- FUNCTION: Reductase component of a two-component system that catalyzes
CC       the first step in the aerobic styrene degradation pathway by
CC       enantioselective epoxidation of the vinyl side chain. Utilizes NADH to
CC       reduce FAD, which is then transferred to the styrene monooxygenase
CC       StyA. {ECO:0000269|PubMed:9172343}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a reduced flavin + NAD(+) = an oxidized flavin + 2 H(+) +
CC         NADH; Xref=Rhea:RHEA:31303, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:60531, ChEBI:CHEBI:62787; EC=1.5.1.36;
CC         Evidence={ECO:0000305|PubMed:9172343};
CC   -!- PATHWAY: Aromatic compound metabolism. {ECO:0000269|PubMed:9172343}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the non-flavoprotein flavin reductase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z92524; CAB06824.1; -; Genomic_DNA.
DR   AlphaFoldDB; O06835; -.
DR   SMR; O06835; -.
DR   BioCyc; MetaCyc:MON-16947; -.
DR   GO; GO:0036382; F:flavin reductase (NADH) activity; IDA:UniProtKB.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0042207; P:styrene catabolic process; IDA:UniProtKB.
DR   Gene3D; 2.30.110.10; -; 1.
DR   InterPro; IPR002563; Flavin_Rdtase-like_dom.
DR   InterPro; IPR012349; Split_barrel_FMN-bd.
DR   Pfam; PF01613; Flavin_Reduct; 1.
DR   SMART; SM00903; Flavin_Reduct; 1.
PE   3: Inferred from homology;
KW   NAD; Oxidoreductase.
FT   CHAIN           1..170
FT                   /note="NADH-dependent flavin reductase StyB"
FT                   /id="PRO_0000430451"
SQ   SEQUENCE   170 AA;  18450 MW;  AAE30A8961B9B3C4 CRC64;
     MTLKKDVAVD IDSTCFRQAV ALFATGIAVL SAETEEGEVH GMTVNSFTSI SLDPPTVMVS
     LKSGRMHELL TRGGRFGVSL LGESQKVFSA FFSKRVMDDT PSPAFTIQAG LPTLRDAMAW
     FECEVESTVQ VHDHTLFIAR VSACGTPEAN APQPLLFFAS RYHGKPLPLN