ID   SUBB_METRA              Reviewed;         173 AA.
AC   E9F5E8;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   04-MAR-2015, sequence version 2.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Terpene cyclase subB {ECO:0000303|PubMed:27189118};
DE            EC=4.2.3.- {ECO:0000305|PubMed:27189118};
DE   AltName: Full=Subglutinol biosynthesis cluster protein B {ECO:0000303|PubMed:27189118};
GN   Name=subB {ECO:0000303|PubMed:27189118}; ORFNames=MAA_07497;
OS   Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) (Metarhizium
OS   anisopliae (strain ARSEF 23)).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium.
OX   NCBI_TaxID=655844;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ARSEF 23 / ATCC MYA-3075;
RX   PubMed=21253567; DOI=10.1371/journal.pgen.1001264;
RA   Gao Q., Jin K., Ying S.-H., Zhang Y., Xiao G., Shang Y., Duan Z., Hu X.,
RA   Xie X.-Q., Zhou G., Peng G., Luo Z., Huang W., Wang B., Fang W., Wang S.,
RA   Zhong Y., Ma L.-J., St Leger R.J., Zhao G.-P., Pei Y., Feng M.-G., Xia Y.,
RA   Wang C.;
RT   "Genome sequencing and comparative transcriptomics of the model
RT   entomopathogenic fungi Metarhizium anisopliae and M. acridum.";
RL   PLoS Genet. 7:E1001264-E1001264(2011).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ARSEF 23 / ATCC MYA-3075;
RX   PubMed=25368161; DOI=10.1073/pnas.1412662111;
RA   Hu X., Xiao G., Zheng P., Shang Y., Su Y., Zhang X., Liu X., Zhan S.,
RA   St Leger R.J., Wang C.;
RT   "Trajectory and genomic determinants of fungal-pathogen speciation and host
RT   adaptation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 111:16796-16801(2014).
RN   [3]
RP   FUNCTION, AND PATHWAY.
RX   PubMed=27189118; DOI=10.1038/ja.2016.54;
RA   Kato H., Tsunematsu Y., Yamamoto T., Namiki T., Kishimoto S., Noguchi H.,
RA   Watanabe K.;
RT   "New natural products isolated from Metarhizium robertsii ARSEF 23 by
RT   chemical screening and identification of the gene cluster through
RT   engineered biosynthesis in Aspergillus nidulans A1145.";
RL   J. Antibiot. 69:561-566(2016).
CC   -!- FUNCTION: Terpene cyclase; part of the gene cluster that mediates the
CC       biosynthesis of the immunosuppressants subglutinols, meroterpenoids
CC       consisting of an alpha-pyrone (4-hydroxy-5,6-dimethyl-2-pyrone) moiety
CC       attached to a decalin core fused to a five-membered cyclic ether
CC       carrying a prenylside chain (PubMed:27189118). The first step of the
CC       pathway is the synthesis of the alpha-pyrone moiety by the polyketide
CC       synthase subA via condensation of one acetyl-CoA starter unit with 3
CC       malonyl-CoA units and 2 methylations (PubMed:27189118). The alpha-
CC       pyrone is then combined with geranylgeranyl pyrophosphate (GGPP) formed
CC       by the GGPP synthase subD through the action of the prenyltransferase
CC       subC to yield a linear alpha-pyrone diterpenoid (PubMed:27189118).
CC       Subsequent steps in the subglutinol biosynthetic pathway involve the
CC       decalin core formation, which is thought to be initiated by the
CC       epoxidation of the C10-C11 olefin by the FAD-dependent oxidoreductase
CC       subE (Probable). The following cyclization cascade would be catalyzed
CC       by the terpene cyclase subB (Probable). Lastly, the FAD-dependent
CC       dehydrogenase subF probably catalyzes the five-membered cyclic ether
CC       formation to complete the formation of subglutinol A (Probable).
CC       Subsequent redox reactions appear to give rise to subglutinol C and D,
CC       however, it remains unclear which enzymes are responsible for these
CC       transformations (Probable). {ECO:0000269|PubMed:27189118,
CC       ECO:0000305|PubMed:27189118}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000269|PubMed:27189118}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the paxB family. {ECO:0000305}.
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DR   EMBL; ADNJ02000014; EFY96951.2; -; Genomic_DNA.
DR   RefSeq; XP_007823686.2; XM_007825495.2.
DR   AlphaFoldDB; E9F5E8; -.
DR   EnsemblFungi; EFY96951; EFY96951; MAA_07497.
DR   GeneID; 19261783; -.
DR   KEGG; maj:MAA_07497; -.
DR   HOGENOM; CLU_087059_3_0_1; -.
DR   UniPathway; UPA00213; -.
DR   Proteomes; UP000002498; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR039020; PaxB-like.
DR   PANTHER; PTHR42038; PTHR42038; 1.
PE   3: Inferred from homology;
KW   Lyase; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..173
FT                   /note="Terpene cyclase subB"
FT                   /id="PRO_0000451338"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        112..132
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        141..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   173 AA;  19296 MW;  F59AF6CFAB6401D2 CRC64;
     MNAADISRAP PGYLEVAWIA DTCKLLMGLG WTTNYAGMIY KSLKDRTYGM ALMPLCCNFA
     WELTYAVIYP FGSRQDKFTH YFGLMLNCGV MYTAVKNAER EWTHAPLVRR NLPFIFIICI
     AAWTTAHLAL ALQIGPSHAQ AFSAYGCQLL LSVGALCQLL SRGSSRGASY FLW