SUBF_BACSU
ID SUBF_BACSU Reviewed; 1433 AA.
AC P16397;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Bacillopeptidase F;
DE EC=3.4.21.-;
DE AltName: Full=90 kDa serine proteinase;
DE AltName: Full=Esterase;
DE AltName: Full=RP-I protease;
DE Flags: Precursor;
GN Name=bpr; Synonyms=bpf; OrderedLocusNames=BSU15300;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 195-222.
RX PubMed=2106512; DOI=10.1128/jb.172.3.1470-1477.1990;
RA Sloma A., Rufo G.A. Jr., Rudolph C.F., Sullivan B.J., Theriault K.A.,
RA Pero J.;
RT "Bacillopeptidase F of Bacillus subtilis: purification of the protein and
RT cloning of the gene.";
RL J. Bacteriol. 172:1470-1477(1990).
RN [2]
RP ERRATUM OF PUBMED:2106512, AND SEQUENCE REVISION.
RX PubMed=2118514; DOI=10.1128/jb.172.9.5520-5521.1990;
RA Sloma A., Rufo G.A. Jr., Rudolph C.F., Sullivan B.J., Theriault K.A.,
RA Pero J.;
RL J. Bacteriol. 172:5520-5521(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2108961; DOI=10.1016/s0021-9258(19)39225-7;
RA Wu X.-C., Nathoo S., Pang A.S.-H., Carne T., Wang S.-L.;
RT "Cloning, genetic organization, and characterization of a structural gene
RT encoding bacillopeptidase F from Bacillus subtilis.";
RL J. Biol. Chem. 265:6845-6850(1990).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-211.
RX PubMed=3139638; DOI=10.1128/jb.170.10.4855-4864.1988;
RA Beall B., Lowe M., Lutkenhaus J.;
RT "Cloning and characterization of Bacillus subtilis homologs of Escherichia
RT coli cell division genes ftsZ and ftsA.";
RL J. Bacteriol. 170:4855-4864(1988).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1410-1433.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=2106671; DOI=10.1093/nar/18.3.657;
RA Masuda E.S., Anaguchi H., Sato T., Takeuchi M., Kobayashi Y.;
RT "Nucleotide sequence of the sporulation gene spoIIGA from Bacillus
RT subtilis.";
RL Nucleic Acids Res. 18:657-657(1990).
RN [7]
RP PROTEIN SEQUENCE OF 195-219.
RC STRAIN=Natto 16;
RX PubMed=1368833; DOI=10.1271/bbb.56.1166;
RA Kato T., Yamagata Y., Arai T., Ichishima E.;
RT "Purification of a new extracellular 90-kDa serine proteinase with
RT isoelectric point of 3.9 from Bacillus subtilis (natto) and elucidation of
RT its distinct mode of action.";
RL Biosci. Biotechnol. Biochem. 56:1166-1168(1992).
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR EMBL; M29035; AAA62679.1; -; Genomic_DNA.
DR EMBL; J05400; AAA83362.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13403.1; -; Genomic_DNA.
DR EMBL; M22630; AAA22458.1; -; Genomic_DNA.
DR EMBL; X17344; CAA35224.1; -; Genomic_DNA.
DR PIR; A36734; A36734.
DR RefSeq; NP_389413.1; NC_000964.3.
DR RefSeq; WP_003245629.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; P16397; -.
DR SMR; P16397; -.
DR STRING; 224308.BSU15300; -.
DR MEROPS; S08.017; -.
DR PaxDb; P16397; -.
DR PRIDE; P16397; -.
DR EnsemblBacteria; CAB13403; CAB13403; BSU_15300.
DR GeneID; 939695; -.
DR KEGG; bsu:BSU15300; -.
DR PATRIC; fig|224308.179.peg.1668; -.
DR eggNOG; COG1404; Bacteria.
DR eggNOG; COG4412; Bacteria.
DR InParanoid; P16397; -.
DR OMA; DEWYRDM; -.
DR BioCyc; BSUB:BSU15300-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd07481; Peptidases_S8_BacillopeptidaseF-like; 1.
DR Gene3D; 2.60.40.10; -; 2.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR033857; Bacillopeptidase_F.
DR InterPro; IPR008969; CarboxyPept-like_regulatory.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR012103; Pept_S8A_Bpr.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PIRSF; PIRSF015477; Bpr; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF49464; SSF49464; 2.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Hydrolase; Protease; Reference proteome;
KW Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..30
FT PROPEP 31..194
FT /evidence="ECO:0000255"
FT /id="PRO_0000027172"
FT CHAIN 195..755
FT /note="Bacillopeptidase F"
FT /id="PRO_0000027173"
FT PROPEP 756..1433
FT /evidence="ECO:0000255"
FT /id="PRO_0000027174"
FT DOMAIN 68..177
FT /note="Inhibitor I9"
FT /evidence="ECO:0000255"
FT DOMAIN 200..512
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT REGION 800..830
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 227
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 274
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 452
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT CONFLICT 219
FT /note="T -> A (in Ref. 7; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 393
FT /note="A -> V (in Ref. 3; AAA83362)"
FT /evidence="ECO:0000305"
FT CONFLICT 829..834
FT /note="KHQNKA -> N (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 836..841
FT /note="QPQVLP -> RTRLYS (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 844..852
FT /note="AQVSVVETG -> FCRSRHKSV (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 853..1433
FT /note="Missing (in Ref. 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1433 AA; 154578 MW; 98DF6846897807C9 CRC64;
MRKKTKNRLI SSVLSTVVIS SLLFPGAAGA SSKVTSPSVK KELQSAESIQ NKISSSLKKS
FKKKEKTTFL IKFKDLANPE KAAKAAVKKA KSKKLSAAKT EYQKRSAVVS SLKVTADESQ
QDVLKYLNTQ KDKGNADQIH SYYVVNGIAV HASKEVMEKV VQFPEVEKVL PNEKRQLFKS
SSPFNMKKAQ KAIKATDGVE WNVDQIDAPK AWALGYDGTG TVVASIDTGV EWNHPALKEK
YRGYNPENPN EPENEMNWYD AVAGEASPYD DLAHGTHVTG TMVGSEPDGT NQIGVAPGAK
WIAVKAFSED GGTDADILEA GEWVLAPKDA EGNPHPEMAP DVVNNSWGGG SGLDEWYRDM
VNAWRAADIF PEFSAGNTDL FIPGGPGSIA NPANYPESFA TGATDINKKL ADFSLQGPSP
YDEIKPEISA PGVNIRSSVP GQTYEDGWDG TSMAGPHVSA VAALLKQANA SLSVDEMEDI
LTSTAEPLTD STFPDSPNNG YGHGLVNAFD AVSAVTDGLG KAEGQVSVEG DDQEPPVYQH
EKVTEAYEGG SLPLTLTAED NVSVTSVKLS YKLDQGEWTE ITAKRISGDH LKGTYQAEIP
DIKGTKLSYK WMIHDFGGHV VSSDVYDVTV KPSITAGYKQ DFETAPGGWV ASGTNNNWEW
GVPSTGPNTA ASGEKVYGTN LTGNYANSAN MNLVMPPIKA PDSGSLFLQF KSWHNLEDDF
DYGYVFVLPE GEKNWEQAGV YNGKTSSWTD EEIDLSAYKG QNIQVMFNLQ SDESIAKEGW
YIDDVVLSDK SAGKTVKKNK LGVEKPSGKQ KKKPVNPKKA KPSANTAVKH QNKAIQPQVL
PLKAQVSVVE TGKSTYSDQS TGQYTLKHKA GDYTLMAEAY GYQSKTQKVS LKTDQTTQAN
FTLEEMKKGT LKGTVINKTT GEPVTGASVY VVEDAAVEPA MTNDKGEYML EAYEGAYTIK
VAAPGYYSDE FSVELKGDVT KETALKPFVG YPGEIAYDDG TAENANSYFA AGNGWAVKMT
LADGKDKGML TGGLFRFWDT EFPDPGGTEF KVEVYDATGK DGAPGKKIAG PFNAEALRNG
EWTKVDLSSK GIMVDKDFYL VYIQSKPDPY SPGLAMDETG QNSGRNWQYI DGKWQPGDKA
DGNYMIRALV DYEAAVPEIT SPTDKSYTNK DSVTVKGNAS PGTTVHIYNG EKEAGETKAA
ADGTFHAGII LNKGENELTA TASTDNGTTD ASSPITVTLD QEKPELTLDN PKDGGKTNKE
TLTVKGAVSD DNLKDVKVNG KKATVADGSY SARILLENGR NEIKVIATDL AGNKTTKKTV
IDVNFDKPVI SGLIPGEDKN LKAGESVKIA FSSAEDLDAT FTIRMPLTNA RASVQNATEL
PLREISPGRY EGYWTATSSI KAKGAKVEVI VRDDYGNETR KTANGKLNMN TEN
