SUBPM_PLAF7
ID SUBPM_PLAF7 Reviewed; 153 AA.
AC C0H4D0;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 1.
DT 25-MAY-2022, entry version 53.
DE RecName: Full=Subtilisin propeptide-like protein {ECO:0000303|PubMed:31942933};
DE AltName: Full=PfSUB1-ProM {ECO:0000303|PubMed:31942933};
DE Flags: Precursor;
GN ORFNames=PF3D7_0507400 {ECO:0000312|EMBL:CAX63952.1};
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329 {ECO:0000312|Proteomes:UP000001450};
RN [1] {ECO:0000312|Proteomes:UP000001450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7 {ECO:0000312|Proteomes:UP000001450};
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [2] {ECO:0000312|Proteomes:UP000001450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7 {ECO:0000312|Proteomes:UP000001450};
RX PubMed=12368867; DOI=10.1038/nature01095;
RA Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D.,
RA Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K.,
RA Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T.,
RA Christodoulou Z., Clark L., Clark R., Corton C., Cronin A., Davies R.M.,
RA Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A.,
RA Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H.,
RA Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D.,
RA Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N.,
RA Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P.,
RA Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A.,
RA Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M.,
RA Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S.,
RA Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R.,
RA Sulston J.E., Craig A., Newbold C., Barrell B.G.;
RT "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.";
RL Nature 419:527-531(2002).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, REGION, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=31942933; DOI=10.1042/bcj20190918;
RA Tarr S.J., Withers-Martinez C., Flynn H.R., Snijders A.P., Masino L.,
RA Koussis K., Conway D.J., Blackman M.J.;
RT "A malaria parasite subtilisin propeptide-like protein is a potent
RT inhibitor of the egress protease SUB1.";
RL Biochem. J. 477:525-540(2020).
CC -!- FUNCTION: Acts as a specific inhibitor of subtilisin-like protease
CC SUB1. {ECO:0000269|PubMed:31942933}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:31942933}.
CC Parasitophorous vacuole lumen {ECO:0000305|PubMed:31942933}. Cell
CC membrane {ECO:0000305|PubMed:31942933}; Peripheral membrane protein
CC {ECO:0000305}; Extracellular side {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the parasite blood stage,
CC specifically in schizonts (at protein level).
CC {ECO:0000269|PubMed:31942933}.
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DR EMBL; AL844504; CAX63952.1; -; Genomic_DNA.
DR RefSeq; XP_002808681.1; XM_002808635.1.
DR AlphaFoldDB; C0H4D0; -.
DR SMR; C0H4D0; -.
DR STRING; 5833.PFE0365c; -.
DR EnsemblProtists; CAX63952; CAX63952; PF3D7_0507400.
DR GeneID; 812880; -.
DR KEGG; pfa:PF3D7_0507400; -.
DR VEuPathDB; PlasmoDB:PF3D7_0507400; -.
DR HOGENOM; CLU_144471_0_0_1; -.
DR InParanoid; C0H4D0; -.
DR OMA; NLRMVIF; -.
DR PhylomeDB; C0H4D0; -.
DR Proteomes; UP000001450; Chromosome 5.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR GO; GO:0010951; P:negative regulation of endopeptidase activity; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..153
FT /note="Subtilisin propeptide-like protein"
FT /evidence="ECO:0000255"
FT /id="PRO_5002898753"
FT REGION 127..153
FT /note="Dispensable for parasite growth in host
FT erythrocytes"
FT /evidence="ECO:0000269|PubMed:31942933"
SQ SEQUENCE 153 AA; 17892 MW; 5F2F406FA26F3167 CRC64;
MKFLFAFNFF SLYIYLYEFL CIHLCGSQVT PAGTVLNSNS ALISRRINRR KMKNCNNNDL
LKVLKMETTY NELPAHNLLM SSKNDINKLF DYINKNEELS KLMNSCGTYV YLKYLGVVIF
SIKENVQISH LSEFIQYLLN KNVCIEFNQN VML
