SUC1_ARATH
ID SUC1_ARATH Reviewed; 513 AA.
AC Q39232;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Sucrose transport protein SUC1;
DE AltName: Full=Sucrose permease 1;
DE AltName: Full=Sucrose-proton symporter 1;
GN Name=SUC1; OrderedLocusNames=At1g71880; ORFNames=F17M19.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP ACTIVITY REGULATION.
RC STRAIN=cv. Columbia;
RX PubMed=7920705; DOI=10.1046/j.1365-313x.1994.6010067.x;
RA Sauer N., Stolz J.;
RT "SUC1 and SUC2: two sucrose transporters from Arabidopsis thaliana;
RT expression and characterization in baker's yeast and identification of the
RT histidine-tagged protein.";
RL Plant J. 6:67-77(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=9307438; DOI=10.1007/s002329900275;
RA Zhou J.-J., Theodoulou F., Sauer N., Sanders D., Miller A.J.;
RT "A kinetic model with ordered cytoplasmic dissociation for SUC1, an
RT Arabidopsis H(+)/sucrose cotransporter expressed in Xenopus oocytes.";
RL J. Membr. Biol. 159:113-125(1997).
RN [7]
RP BIOPHYSICOCHEMICAL PROPERTIES, ACTIVITY REGULATION, AND MUTAGENESIS OF
RP HIS-65.
RX PubMed=9671798; DOI=10.1073/pnas.95.15.9025;
RA Lu J.M.-Y., Bush D.R.;
RT "His-65 in the proton-sucrose symporter is an essential amino acid whose
RT modification with site-directed mutagenesis increases transport activity.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:9025-9030(1998).
RN [8]
RP FUNCTION, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=10476074; DOI=10.1046/j.1365-313x.1999.00527.x;
RA Stadler R., Truernit E., Gahrtz M., Sauer N.;
RT "The AtSUC1 sucrose carrier may represent the osmotic driving force for
RT anther dehiscence and pollen tube growth in Arabidopsis.";
RL Plant J. 19:269-278(1999).
RN [9]
RP INDUCTION.
RX PubMed=11673631; DOI=10.1093/pcp/pce150;
RA Furuichi T., Mori I.C., Takahashi K., Muto S.;
RT "Sugar-induced increase in cytosolic Ca(2+) in Arabidopsis thaliana whole
RT plants.";
RL Plant Cell Physiol. 42:1149-1155(2001).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Seedling;
RX PubMed=17586839; DOI=10.1074/mcp.m700164-mcp200;
RA Niittylae T., Fuglsang A.T., Palmgren M.G., Frommer W.B., Schulze W.X.;
RT "Temporal analysis of sucrose-induced phosphorylation changes in plasma
RT membrane proteins of Arabidopsis.";
RL Mol. Cell. Proteomics 6:1711-1726(2007).
RN [11]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18359840; DOI=10.1104/pp.108.118992;
RA Sivitz A.B., Reinders A., Ward J.M.;
RT "Arabidopsis sucrose transporter AtSUC1 is important for pollen germination
RT and sucrose-induced anthocyanin accumulation.";
RL Plant Physiol. 147:92-100(2008).
CC -!- FUNCTION: Responsible for the transport of sucrose into the cell, with
CC the concomitant uptake of protons (symport system). This transport is
CC both voltage- and energy-dependent. Can also transport other glucosides
CC such as maltose, alpha-phenylglucoside and beta-phenylglucoside. May
CC also transport biotin. Required for normal pollen germination and
CC anthocyanin accumulation induced by sucrose.
CC {ECO:0000269|PubMed:10476074, ECO:0000269|PubMed:18359840,
CC ECO:0000269|PubMed:7920705, ECO:0000269|PubMed:9307438}.
CC -!- ACTIVITY REGULATION: Inhibited by DEPC, protonophores (e.g.
CC dinitrophenol and carbonyl cyanide m-chlorophenyl-hydrazone (CCCP)),
CC and SH group inhibitors (e.g. N-ethylmaleimide (NEM) and p-
CC chloromercuriphenyl sulphonic acid (PCMPS)).
CC {ECO:0000269|PubMed:7920705, ECO:0000269|PubMed:9671798}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=450 uM for sucrose (at pH 5.5 and 30 degrees Celsius)
CC {ECO:0000269|PubMed:7920705, ECO:0000269|PubMed:9671798};
CC Vmax=45 umol/h/g enzyme (without glucose)
CC {ECO:0000269|PubMed:7920705, ECO:0000269|PubMed:9671798};
CC Vmax=154 umol/h/g enzyme (in the presence of 10 mM glucose)
CC {ECO:0000269|PubMed:7920705, ECO:0000269|PubMed:9671798};
CC pH dependence:
CC Optimum pH is 5-6. {ECO:0000269|PubMed:7920705,
CC ECO:0000269|PubMed:9671798};
CC -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers (at protein level). Highly
CC expressed in pollen. Expressed in pollen tubes and root vascular
CC cylinder, pericycle and endodermis. {ECO:0000269|PubMed:10476074,
CC ECO:0000269|PubMed:18359840}.
CC -!- DEVELOPMENTAL STAGE: Transcripts accumulate in mature pollen before and
CC during germination, but translation starts only when pollen germination
CC initiates, and continues in pollen tubes. Expressed in cells
CC surrounding the vascular bundle of the anther connective tissue, mostly
CC at the dehiscence time. Also present in a ring of parenchymatic cells
CC between the xylem vessels of the style (upper end of the transmitting
CC tract toward which pollen tubes grow). Expressed in the epidermal cell
CC layers of funiculi (at protein level). {ECO:0000269|PubMed:10476074}.
CC -!- INDUCTION: By exogenous sucrose in roots. Induced by sucrose depletion.
CC {ECO:0000269|PubMed:11673631, ECO:0000269|PubMed:18359840}.
CC -!- DISRUPTION PHENOTYPE: Defective pollen with low rate of germination.
CC Reduction of anthocyanin accumulation in response to exogenous sucrose
CC or maltose. {ECO:0000269|PubMed:18359840}.
CC -!- SIMILARITY: Belongs to the glycoside-pentoside-hexuronide (GPH) cation
CC symporter transporter (TC 2.A.2.4) family. {ECO:0000305}.
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DR EMBL; X75365; CAA53147.1; -; mRNA.
DR EMBL; AC021665; AAG52225.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35247.1; -; Genomic_DNA.
DR EMBL; AY049275; AAK83617.1; -; mRNA.
DR EMBL; BT020416; AAV97807.1; -; mRNA.
DR PIR; S38197; S38197.
DR RefSeq; NP_177333.1; NM_105846.4.
DR AlphaFoldDB; Q39232; -.
DR BioGRID; 28739; 6.
DR STRING; 3702.AT1G71880.1; -.
DR TCDB; 2.A.2.4.1; the glycoside-pentoside-hexuronide (gph):cation symporter family.
DR iPTMnet; Q39232; -.
DR PaxDb; Q39232; -.
DR PRIDE; Q39232; -.
DR ProteomicsDB; 245227; -.
DR EnsemblPlants; AT1G71880.1; AT1G71880.1; AT1G71880.
DR GeneID; 843519; -.
DR Gramene; AT1G71880.1; AT1G71880.1; AT1G71880.
DR KEGG; ath:AT1G71880; -.
DR Araport; AT1G71880; -.
DR TAIR; locus:2016069; AT1G71880.
DR eggNOG; KOG0637; Eukaryota.
DR HOGENOM; CLU_025234_3_0_1; -.
DR InParanoid; Q39232; -.
DR OMA; GIAWAGM; -.
DR OrthoDB; 1230185at2759; -.
DR PhylomeDB; Q39232; -.
DR BioCyc; MetaCyc:AT1G71880-MON; -.
DR SABIO-RK; Q39232; -.
DR UniPathway; UPA00238; -.
DR PRO; PR:Q39232; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q39232; baseline and differential.
DR Genevisible; Q39232; AT.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005773; C:vacuole; HDA:TAIR.
DR GO; GO:0008506; F:sucrose:proton symporter activity; IDA:TAIR.
DR GO; GO:0009846; P:pollen germination; IMP:TAIR.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR GO; GO:0005985; P:sucrose metabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005989; Suc_symporter_pln.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR01301; GPH_sucrose; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Sugar transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..513
FT /note="Sucrose transport protein SUC1"
FT /id="PRO_0000122522"
FT TOPO_DOM 1..32
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..67
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 89..101
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..139
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..178
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..224
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 246..282
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 304..334
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 356..365
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 387..408
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 430..441
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..474
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 475..495
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 496..513
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 20
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17586839"
FT MUTAGEN 65
FT /note="H->C: No sucrose transport activity, high
FT instability."
FT /evidence="ECO:0000269|PubMed:9671798"
FT MUTAGEN 65
FT /note="H->D,G,L,Y: Reduced sucrose transport activity."
FT /evidence="ECO:0000269|PubMed:9671798"
FT MUTAGEN 65
FT /note="H->K: Enhanced sucrose transport activity."
FT /evidence="ECO:0000269|PubMed:9671798"
FT MUTAGEN 65
FT /note="H->Q: Reduced sensitivity to DEPC."
FT /evidence="ECO:0000269|PubMed:9671798"
FT MUTAGEN 65
FT /note="H->R: Enhanced sucrose transport activity, reduced
FT sensitivity to DEPC."
FT /evidence="ECO:0000269|PubMed:9671798"
FT MUTAGEN 65
FT /note="H->S: Reduced sucrose transport activity, reduced
FT sensitivity to DEPC."
FT /evidence="ECO:0000269|PubMed:9671798"
SQ SEQUENCE 513 AA; 54858 MW; 3F44FE0B318D5250 CRC64;
MGAYETEKPT KDAAALETQS PEDFDQPSPL RKIISVASIA AGVQFGWALQ LSLLTPYVQL
LGIPHKWSSL IWLCGPVSGM IVQPIVGFHS DRCRSKFGRR RPFIATGAAL VAVAVFLIGY
AADFGYKMGD KLEEKVKVRA IGIFALGFWI LDVANNTLQG PCRAFLADLA AGDAKRTRVA
NAFFSFFMAV GNVLGYAAGS YTNLHKMFPF TMTKACDIYC ANLKTCFFLS ITLLLIVTVT
SLWYVNDKQW SPPPRNADDD EKTSSVPLFG EIFGAFKVMK RPMWMLLIVT ALNWIAWFPF
LLFDTDWMGR EVFGGDSDGN ERSKKLYSLG VQSGAMGLMF NSIVLGFMSL GVEWIGRKLG
GAKRLWGIVN FILAAGLAMT VLVTKFAEDH RKTAGDLAGP SASVKAGALS LFAVLGIPLA
ITFSTPFALA SIFSSCSGAG QGLSLGVLNL AIVIPQMIVS LGGGPFDALF GGGNLPAFIV
AAIAAAISGV LALTVLPSPP PDAPKATTMG GFH
