SUC8_ARATH
ID SUC8_ARATH Reviewed; 492 AA.
AC Q9ZVK6;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Sucrose transport protein SUC8;
DE AltName: Full=Sucrose permease 8;
DE AltName: Full=Sucrose-proton symporter 8;
GN Name=SUC8; OrderedLocusNames=At2g14670; ORFNames=T6B13.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, AND ACTIVITY
RP REGULATION.
RX PubMed=15361146; DOI=10.1111/j.1365-313x.2004.02196.x;
RA Sauer N., Ludwig A., Knoblauch A., Rothe P., Gahrtz M., Klebl F.;
RT "AtSUC8 and AtSUC9 encode functional sucrose transporters, but the closely
RT related AtSUC6 and AtSUC7 genes encode aberrant proteins in different
RT Arabidopsis ecotypes.";
RL Plant J. 40:120-130(2004).
CC -!- FUNCTION: Responsible for the transport of sucrose into the cell, with
CC the concomitant uptake of protons (symport system). Can also transport
CC maltose at a lesser rate. May also transport biotin.
CC {ECO:0000269|PubMed:15361146}.
CC -!- ACTIVITY REGULATION: Inhibited by protonophores (e.g. carbonyl cyanide
CC m-chlorophenyl-hydrazone (CCCP)), but not by SH group inhibitors (e.g.
CC p-chloromercuribenzene sulphonic acid (PCMBS)).
CC {ECO:0000269|PubMed:15361146}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=150 uM for sucrose {ECO:0000269|PubMed:15361146};
CC -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in flowers, along the
CC transmitting tissue and at the funiculi. {ECO:0000269|PubMed:15361146}.
CC -!- SIMILARITY: Belongs to the glycoside-pentoside-hexuronide (GPH) cation
CC symporter transporter (TC 2.A.2.4) family. {ECO:0000305}.
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DR EMBL; AC005398; AAC69375.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06318.1; -; Genomic_DNA.
DR PIR; A84520; A84520.
DR RefSeq; NP_179074.1; NM_127031.1.
DR AlphaFoldDB; Q9ZVK6; -.
DR STRING; 3702.AT2G14670.1; -.
DR PaxDb; Q9ZVK6; -.
DR PRIDE; Q9ZVK6; -.
DR ProteomicsDB; 245230; -.
DR EnsemblPlants; AT2G14670.1; AT2G14670.1; AT2G14670.
DR GeneID; 815955; -.
DR Gramene; AT2G14670.1; AT2G14670.1; AT2G14670.
DR KEGG; ath:AT2G14670; -.
DR Araport; AT2G14670; -.
DR TAIR; locus:2064253; AT2G14670.
DR eggNOG; KOG0637; Eukaryota.
DR HOGENOM; CLU_025234_3_0_1; -.
DR InParanoid; Q9ZVK6; -.
DR OMA; EFAGPTG; -.
DR OrthoDB; 1230185at2759; -.
DR PhylomeDB; Q9ZVK6; -.
DR UniPathway; UPA00238; -.
DR PRO; PR:Q9ZVK6; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZVK6; baseline and differential.
DR Genevisible; Q9ZVK6; AT.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0008506; F:sucrose:proton symporter activity; IBA:GO_Central.
DR GO; GO:0005985; P:sucrose metabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005989; Suc_symporter_pln.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR01301; GPH_sucrose; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Phosphoprotein; Reference proteome;
KW Sugar transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..492
FT /note="Sucrose transport protein SUC8"
FT /id="PRO_0000122529"
FT TOPO_DOM 1..33
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..68
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 90..101
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..139
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..181
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 203..224
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 246..277
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 299..324
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 325..345
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 346..359
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..380
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 381..403
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 404..424
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 425..444
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 445..465
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 466..469
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 470..490
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 491..492
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 17
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q39232"
SQ SEQUENCE 492 AA; 52748 MW; 5921D297A6B2EF75 CRC64;
MSDLQAKNDV VAVDRQSSSS LADLDGPSPL RKMISVASIA AGIQFGWALQ LSLLTPYVQL
LGVPHKWSSF IWLCGPVSGL LVQPSVGYFS DRCTSRFGRR RPFIATGALL VAVAVVLIGY
AADFGHSMGD KIDKPVKMRA VVIFALGFWI LDVANNTLQG PCRAFLGDLA AGDAKKTRTA
NAFFSFFMAV GNVLGYAAGS YTNLYKIFPF TMTKACDIYC ANLKSCFFLS ITLLLVVTII
ALWYVEDKQW SPKADSDNEK TPFFGEIFGA FKVMKRPMWM LLIVTALNWI AWFPFLLYDT
DWMGREVYGG DSKGDDKMKK LYNQGIHVGA LGLMLNSIVL GIVSLGIEGI SKKIGGAKRL
WGAVNIILAV CLAMTVLVTK KAEEHRRIAG PMALPTDGIR AGALTLFALL GIPLAITFSI
PFALASIISS SSGAGQGLSL GVLNMAIVIP QMIVSFGVGP IDALFGGGNL PRFVVGAIAA
AISSVVAFTV LP
