SUCHY_MOUSE
ID SUCHY_MOUSE Reviewed; 436 AA.
AC Q7TNE1; G3X9F8;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Succinate--hydroxymethylglutarate CoA-transferase;
DE EC=2.8.3.13;
DE AltName: Full=SuccinylCoA:glutarate-CoA transferase;
DE Flags: Precursor;
GN Name=Sugct;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-392 AND LYS-423, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=23576753; DOI=10.1073/pnas.1302961110;
RA Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B.,
RA Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.;
RT "Label-free quantitative proteomics of the lysine acetylome in mitochondria
RT identifies substrates of SIRT3 in metabolic pathways.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013).
CC -!- FUNCTION: Catalyzes the succinyl-CoA-dependent conversion of glutarate
CC to glutaryl-CoA. Can use different dicarboxylic acids as CoA acceptors,
CC the preferred ones are glutarate, succinate, adipate, and 3-
CC hydroxymethylglutarate (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxy-3-methylglutarate + succinyl-CoA = (3S)-hydroxy-3-
CC methylglutaryl-CoA + succinate; Xref=Rhea:RHEA:12284,
CC ChEBI:CHEBI:17325, ChEBI:CHEBI:30031, ChEBI:CHEBI:43074,
CC ChEBI:CHEBI:57292; EC=2.8.3.13;
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CoA-transferase III family. {ECO:0000305}.
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DR EMBL; AC154219; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC154317; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC154368; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC154386; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC154662; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC156459; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC156569; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CT030714; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466561; EDL32726.1; -; Genomic_DNA.
DR EMBL; BC055852; AAH55852.1; -; mRNA.
DR CCDS; CCDS26252.1; -.
DR RefSeq; NP_619595.3; NM_138654.3.
DR AlphaFoldDB; Q7TNE1; -.
DR SMR; Q7TNE1; -.
DR BioGRID; 228644; 1.
DR STRING; 10090.ENSMUSP00000070759; -.
DR iPTMnet; Q7TNE1; -.
DR PhosphoSitePlus; Q7TNE1; -.
DR jPOST; Q7TNE1; -.
DR MaxQB; Q7TNE1; -.
DR PaxDb; Q7TNE1; -.
DR PRIDE; Q7TNE1; -.
DR ProteomicsDB; 254780; -.
DR Antibodypedia; 49926; 128 antibodies from 20 providers.
DR DNASU; 192136; -.
DR Ensembl; ENSMUST00000068545; ENSMUSP00000070759; ENSMUSG00000055137.
DR GeneID; 192136; -.
DR KEGG; mmu:192136; -.
DR UCSC; uc007pnx.3; mouse.
DR CTD; 79783; -.
DR MGI; MGI:1923221; Sugct.
DR VEuPathDB; HostDB:ENSMUSG00000055137; -.
DR eggNOG; KOG3957; Eukaryota.
DR GeneTree; ENSGT00940000157866; -.
DR HOGENOM; CLU_033975_2_1_1; -.
DR InParanoid; Q7TNE1; -.
DR OMA; IIAGPYC; -.
DR OrthoDB; 983223at2759; -.
DR PhylomeDB; Q7TNE1; -.
DR TreeFam; TF314188; -.
DR BioGRID-ORCS; 192136; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Sugct; mouse.
DR PRO; PR:Q7TNE1; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q7TNE1; protein.
DR Bgee; ENSMUSG00000055137; Expressed in right kidney and 110 other tissues.
DR ExpressionAtlas; Q7TNE1; baseline and differential.
DR Genevisible; Q7TNE1; MM.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0047369; F:succinate-hydroxymethylglutarate CoA-transferase activity; ISS:UniProtKB.
DR Gene3D; 3.30.1540.10; -; 1.
DR Gene3D; 3.40.50.10540; -; 1.
DR InterPro; IPR003673; CoA-Trfase_fam_III.
DR InterPro; IPR044855; CoA-Trfase_III_dom3_sf.
DR InterPro; IPR023606; CoA-Trfase_III_dom_1_sf.
DR Pfam; PF02515; CoA_transf_3; 1.
DR SUPFAM; SSF89796; SSF89796; 1.
PE 1: Evidence at protein level;
KW Acetylation; Mitochondrion; Reference proteome; Transferase;
KW Transit peptide.
FT TRANSIT 1..8
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 9..436
FT /note="Succinate--hydroxymethylglutarate CoA-transferase"
FT /id="PRO_0000194727"
FT ACT_SITE 203
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT MOD_RES 392
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23576753"
FT MOD_RES 423
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23576753"
FT CONFLICT 305
FT /note="C -> S (in Ref. 1; AAH55852)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 436 AA; 47690 MW; 7246B53BBF2ECBD7 CRC64;
MLWMLARAVA FRRPGRGLAG GRGLWTGRPQ SDCDSMKPLE GVRILDLTRV LAGPFATMNL
GDLGAEVIKV ERPGAGDDTR SWGPPFVNTE STYFLSVNRN KKSIAVNIKD PRGVRIVKEL
AAICDVFVEN YVPGKLSEMG LGYEDIDKIA PHIIYCSITG YGQTGPMSHR AGYDAIASAM
SGLMHITGPE DGDPVRPGVA MTDLATGLFA YGAIMAGLLQ RYRTGKGLFI DCNLLSSQVA
CLTQVAANYL IGQKEAKRWG TAHGSIVPYQ AFKTKDGYLV IGAGNNQQFA VVCKILNLPE
LIDDCKYRTN HLRVQNRKEL VKILSARFAE EVTAKWLCLF EGSGIPYGPI NSLKDVFSEA
QVLHNGLVME MNHPTVGKIS VPGPAVRYSK FKMSEAKPPP LLGQHTRHIL KEVLRYDEGA
IEKLLCSGVI EQHETK
