SUCR1_HUMAN
ID SUCR1_HUMAN Reviewed; 334 AA.
AC Q9BXA5; A8K305; Q8TDQ8;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Succinate receptor 1;
DE AltName: Full=G-protein coupled receptor 91;
DE AltName: Full=P2Y purinoceptor 1-like;
GN Name=SUCNR1; Synonyms=GPR91;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=11273702; DOI=10.1006/jmbi.2001.4520;
RA Wittenberger T., Schaller H.C., Hellebrand S.;
RT "An expressed sequence tag (EST) data mining strategy succeeding in the
RT discovery of new G-protein coupled receptors.";
RL J. Mol. Biol. 307:799-813(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Zhang W., Li N., Wan T., Cao X.;
RT "Human P2Y purinoceptor 1.";
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Kidney;
RA Kaighin V.A., Martin A.L., Aronstam R.S.;
RL Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Umbilical cord blood;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP FUNCTION, MUTAGENESIS OF ARG-99; HIS-103; TYR-107; HIS-249; ARG-252;
RP ARG-255 AND ARG-281, AND SUBCELLULAR LOCATION.
RX PubMed=15141213; DOI=10.1038/nature02488;
RA He W., Miao F.J.-P., Lin D.C.-H., Schwandner R.T., Wang Z., Gao J.,
RA Chen J.-L., Tian H., Ling L.;
RT "Citric acid cycle intermediates as ligands for orphan G-protein-coupled
RT receptors.";
RL Nature 429:188-193(2004).
CC -!- FUNCTION: Receptor for succinate. {ECO:0000269|PubMed:15141213}.
CC -!- INTERACTION:
CC Q9BXA5; P13473-2: LAMP2; NbExp=3; IntAct=EBI-17962797, EBI-21591415;
CC Q9BXA5; Q9Y371: SH3GLB1; NbExp=3; IntAct=EBI-17962797, EBI-2623095;
CC Q9BXA5; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-17962797, EBI-8638294;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15141213};
CC Multi-pass membrane protein {ECO:0000269|PubMed:15141213}.
CC -!- TISSUE SPECIFICITY: Expressed specifically in kidney.
CC {ECO:0000269|PubMed:11273702}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-5 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK29080.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF348078; AAK29080.1; ALT_INIT; mRNA.
DR EMBL; AF247785; AAL95690.1; -; mRNA.
DR EMBL; EU432110; ABY87909.1; -; mRNA.
DR EMBL; AK290420; BAF83109.1; -; mRNA.
DR EMBL; AC068647; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471052; EAW78789.1; -; Genomic_DNA.
DR EMBL; BC030948; AAH30948.3; -; mRNA.
DR CCDS; CCDS3162.1; -.
DR RefSeq; NP_149039.2; NM_033050.5.
DR AlphaFoldDB; Q9BXA5; -.
DR SMR; Q9BXA5; -.
DR BioGRID; 121179; 5.
DR IntAct; Q9BXA5; 3.
DR STRING; 9606.ENSP00000355156; -.
DR BindingDB; Q9BXA5; -.
DR ChEMBL; CHEMBL2150838; -.
DR DrugBank; DB00139; Succinic acid.
DR DrugCentral; Q9BXA5; -.
DR GuidetoPHARMACOLOGY; 166; -.
DR GlyGen; Q9BXA5; 2 sites.
DR PhosphoSitePlus; Q9BXA5; -.
DR BioMuta; SUCNR1; -.
DR DMDM; 259016146; -.
DR MassIVE; Q9BXA5; -.
DR PaxDb; Q9BXA5; -.
DR PeptideAtlas; Q9BXA5; -.
DR PRIDE; Q9BXA5; -.
DR ABCD; Q9BXA5; 1 sequenced antibody.
DR Antibodypedia; 18315; 266 antibodies from 28 providers.
DR DNASU; 56670; -.
DR Ensembl; ENST00000362032.6; ENSP00000355156.4; ENSG00000198829.6.
DR GeneID; 56670; -.
DR KEGG; hsa:56670; -.
DR MANE-Select; ENST00000362032.6; ENSP00000355156.4; NM_033050.6; NP_149039.2.
DR UCSC; uc003ezf.3; human.
DR CTD; 56670; -.
DR DisGeNET; 56670; -.
DR GeneCards; SUCNR1; -.
DR HGNC; HGNC:4542; SUCNR1.
DR HPA; ENSG00000198829; Tissue enriched (kidney).
DR MIM; 606381; gene.
DR neXtProt; NX_Q9BXA5; -.
DR OpenTargets; ENSG00000198829; -.
DR PharmGKB; PA28934; -.
DR VEuPathDB; HostDB:ENSG00000198829; -.
DR eggNOG; ENOG502QVWP; Eukaryota.
DR GeneTree; ENSGT01030000234621; -.
DR HOGENOM; CLU_009579_8_2_1; -.
DR InParanoid; Q9BXA5; -.
DR OMA; VMCFFYY; -.
DR OrthoDB; 1133445at2759; -.
DR PhylomeDB; Q9BXA5; -.
DR TreeFam; TF350009; -.
DR PathwayCommons; Q9BXA5; -.
DR Reactome; R-HSA-373076; Class A/1 (Rhodopsin-like receptors).
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR SignaLink; Q9BXA5; -.
DR BioGRID-ORCS; 56670; 17 hits in 1071 CRISPR screens.
DR ChiTaRS; SUCNR1; human.
DR GeneWiki; SUCNR1; -.
DR GenomeRNAi; 56670; -.
DR Pharos; Q9BXA5; Tchem.
DR PRO; PR:Q9BXA5; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q9BXA5; protein.
DR Bgee; ENSG00000198829; Expressed in kidney epithelium and 101 other tissues.
DR Genevisible; Q9BXA5; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR GO; GO:0038023; F:signaling receptor activity; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0042593; P:glucose homeostasis; IEA:Ensembl.
DR GO; GO:0002281; P:macrophage activation involved in immune response; IEA:Ensembl.
DR GO; GO:0050921; P:positive regulation of chemotaxis; IEA:Ensembl.
DR GO; GO:0050729; P:positive regulation of inflammatory response; IEA:Ensembl.
DR GO; GO:0060177; P:regulation of angiotensin metabolic process; IEA:Ensembl.
DR GO; GO:0002001; P:renin secretion into blood stream; IEA:Ensembl.
DR GO; GO:0051592; P:response to calcium ion; IEA:Ensembl.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..334
FT /note="Succinate receptor 1"
FT /id="PRO_0000070134"
FT TOPO_DOM 5..31
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..59
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..103
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..137
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..185
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..230
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..251
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 252..281
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 303..334
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 8
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 168
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 95..172
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT MUTAGEN 99
FT /note="R->A: Abolishes activation by succinate."
FT /evidence="ECO:0000269|PubMed:15141213"
FT MUTAGEN 103
FT /note="H->A: Abolishes activation by succinate."
FT /evidence="ECO:0000269|PubMed:15141213"
FT MUTAGEN 107
FT /note="Y->F: No effect on receptor function."
FT /evidence="ECO:0000269|PubMed:15141213"
FT MUTAGEN 249
FT /note="H->A: No effect on receptor function."
FT /evidence="ECO:0000269|PubMed:15141213"
FT MUTAGEN 252
FT /note="R->A: Abolishes activation by succinate."
FT /evidence="ECO:0000269|PubMed:15141213"
FT MUTAGEN 255
FT /note="R->A: No effect on receptor function."
FT /evidence="ECO:0000269|PubMed:15141213"
FT MUTAGEN 281
FT /note="R->A: Abolishes activation by succinate."
FT /evidence="ECO:0000269|PubMed:15141213"
SQ SEQUENCE 334 AA; 38698 MW; 33146E1AD87F0E81 CRC64;
MLGIMAWNAT CKNWLAAEAA LEKYYLSIFY GIEFVVGVLG NTIVVYGYIF SLKNWNSSNI
YLFNLSVSDL AFLCTLPMLI RSYANGNWIY GDVLCISNRY VLHANLYTSI LFLTFISIDR
YLIIKYPFRE HLLQKKEFAI LISLAIWVLV TLELLPILPL INPVITDNGT TCNDFASSGD
PNYNLIYSMC LTLLGFLIPL FVMCFFYYKI ALFLKQRNRQ VATALPLEKP LNLVIMAVVI
FSVLFTPYHV MRNVRIASRL GSWKQYQCTQ VVINSFYIVT RPLAFLNSVI NPVFYFLLGD
HFRDMLMNQL RHNFKSLTSF SRWAHELLLS FREK
