SUFB_ECOLI
ID SUFB_ECOLI Reviewed; 495 AA.
AC P77522;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=FeS cluster assembly protein SufB;
GN Name=sufB; Synonyms=ynhE; OrderedLocusNames=b1683, JW5273;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP GENE NAME.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=10322040; DOI=10.1128/jb.181.10.3307-3309.1999;
RA Patzer S.I., Hantke K.;
RT "SufS is a NifS-like protein, and SufD is necessary for stability of the
RT 2Fe-2S FhuF protein in Escherichia coli.";
RL J. Bacteriol. 181:3307-3309(1999).
RN [5]
RP FUNCTION, AND SUBUNIT.
RX PubMed=12941942; DOI=10.1074/jbc.m308004200;
RA Outten F.W., Wood M.J., Munoz F.M., Storz G.;
RT "The SufE protein and the SufBCD complex enhance SufS cysteine desulfurase
RT activity as part of a sulfur transfer pathway for Fe-S cluster assembly in
RT Escherichia coli.";
RL J. Biol. Chem. 278:45713-45719(2003).
RN [6]
RP FUNCTION, AND INTERACTION WITH SUFA.
RX PubMed=19810706; DOI=10.1021/bi901518y;
RA Chahal H.K., Dai Y., Saini A., Ayala-Castro C., Outten F.W.;
RT "The SufBCD Fe-S scaffold complex interacts with SufA for Fe-S cluster
RT transfer.";
RL Biochemistry 48:10644-10653(2009).
CC -!- FUNCTION: The SufBCD complex acts synergistically with SufE to
CC stimulate the cysteine desulfurase activity of SufS. The SufBCD complex
CC contributes to the assembly or repair of oxygen-labile iron-sulfur
CC clusters under oxidative stress. May facilitate iron uptake from
CC extracellular iron chelators under iron limitation.
CC {ECO:0000269|PubMed:12941942, ECO:0000269|PubMed:19810706}.
CC -!- SUBUNIT: Part of the SufBCD complex that contains SufB, SufC and SufD
CC (PubMed:12941942). Interacts with SufA (PubMed:19810706).
CC {ECO:0000269|PubMed:12941942, ECO:0000269|PubMed:19810706}.
CC -!- INTERACTION:
CC P77522; P77667: sufA; NbExp=5; IntAct=EBI-562758, EBI-1125011;
CC P77522; P77499: sufC; NbExp=15; IntAct=EBI-562758, EBI-561601;
CC P77522; P77689: sufD; NbExp=15; IntAct=EBI-562758, EBI-562751;
CC -!- SIMILARITY: Belongs to the UPF0051 (ycf24) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U00096; AAC74753.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15454.2; -; Genomic_DNA.
DR PIR; C64926; C64926.
DR RefSeq; NP_416198.2; NC_000913.3.
DR RefSeq; WP_000089364.1; NZ_STEB01000003.1.
DR PDB; 5AWF; X-ray; 2.96 A; A/E=1-495.
DR PDB; 5AWG; X-ray; 4.28 A; A/E=1-495.
DR PDBsum; 5AWF; -.
DR PDBsum; 5AWG; -.
DR AlphaFoldDB; P77522; -.
DR SMR; P77522; -.
DR BioGRID; 4260282; 68.
DR BioGRID; 850120; 1.
DR ComplexPortal; CPX-2123; sufBCD complex.
DR DIP; DIP-10938N; -.
DR IntAct; P77522; 5.
DR STRING; 511145.b1683; -.
DR jPOST; P77522; -.
DR PaxDb; P77522; -.
DR PRIDE; P77522; -.
DR DNASU; 945753; -.
DR EnsemblBacteria; AAC74753; AAC74753; b1683.
DR EnsemblBacteria; BAA15454; BAA15454; BAA15454.
DR GeneID; 66674424; -.
DR GeneID; 945753; -.
DR KEGG; ecj:JW5273; -.
DR KEGG; eco:b1683; -.
DR PATRIC; fig|1411691.4.peg.575; -.
DR EchoBASE; EB3723; -.
DR eggNOG; COG0719; Bacteria.
DR HOGENOM; CLU_026231_0_0_6; -.
DR InParanoid; P77522; -.
DR OMA; YYSAPKQ; -.
DR PhylomeDB; P77522; -.
DR BioCyc; EcoCyc:G6909-MON; -.
DR PRO; PR:P77522; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:1990229; C:iron-sulfur cluster assembly complex; IDA:EcoCyc.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IDA:EcoCyc.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IDA:EcoCyc.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IMP:EcoCyc.
DR DisProt; DP01920; -.
DR InterPro; IPR010231; SUF_FeS_clus_asmbl_SufB.
DR InterPro; IPR000825; SUF_FeS_clus_asmbl_SufBD.
DR InterPro; IPR037284; SUF_FeS_clus_asmbl_SufBD_sf.
DR InterPro; IPR045595; SufBD_N.
DR Pfam; PF01458; SUFBD; 1.
DR Pfam; PF19295; SufBD_N; 1.
DR SUPFAM; SSF101960; SSF101960; 1.
DR TIGRFAMs; TIGR01980; sufB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome.
FT CHAIN 1..495
FT /note="FeS cluster assembly protein SufB"
FT /id="PRO_0000147373"
FT HELIX 40..46
FT /evidence="ECO:0007829|PDB:5AWF"
FT HELIX 54..69
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 165..168
FT /evidence="ECO:0007829|PDB:5AWF"
FT HELIX 169..175
FT /evidence="ECO:0007829|PDB:5AWF"
FT HELIX 177..183
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 186..188
FT /evidence="ECO:0007829|PDB:5AWF"
FT HELIX 194..200
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 206..211
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 234..242
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 247..257
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 264..273
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 278..288
FT /evidence="ECO:0007829|PDB:5AWF"
FT TURN 290..292
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 298..307
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 313..321
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 324..328
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 331..334
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 340..349
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 354..357
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 360..363
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 369..379
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 383..392
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 400..413
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 415..424
FT /evidence="ECO:0007829|PDB:5AWF"
FT STRAND 430..439
FT /evidence="ECO:0007829|PDB:5AWF"
FT HELIX 442..450
FT /evidence="ECO:0007829|PDB:5AWF"
FT HELIX 455..466
FT /evidence="ECO:0007829|PDB:5AWF"
FT HELIX 468..471
FT /evidence="ECO:0007829|PDB:5AWF"
FT HELIX 476..493
FT /evidence="ECO:0007829|PDB:5AWF"
SQ SEQUENCE 495 AA; 54745 MW; 3BB49EEADB6C1278 CRC64;
MSRNTEATDD VKTWTGGPLN YKEGFFTQLA TDELAKGINE EVVRAISAKR NEPEWMLEFR
LNAYRAWLEM EEPHWLKAHY DKLNYQDYSY YSAPSCGNCD DTCASEPGAV QQTGANAFLS
KEVEAAFEQL GVPVREGKEV AVDAIFDSVS VATTYREKLA EQGIIFCSFG EAIHDHPELV
RKYLGTVVPG NDNFFAALNA AVASDGTFIY VPKGVRCPME LSTYFRINAE KTGQFERTIL
VADEDSYVSY IEGCSAPVRD SYQLHAAVVE VIIHKNAEVK YSTVQNWFPG DNNTGGILNF
VTKRALCEGE NSKMSWTQSE TGSAITWKYP SCILRGDNSI GEFYSVALTS GHQQADTGTK
MIHIGKNTKS TIISKGISAG HSQNSYRGLV KIMPTATNAR NFTQCDSMLI GANCGAHTFP
YVECRNNSAQ LEHEATTSRI GEDQLFYCLQ RGISEEDAIS MIVNGFCKDV FSELPLEFAV
EAQKLLAISL EHSVG
