SUF_DROME
ID SUF_DROME Reviewed; 765 AA.
AC P25991; A0A023GQK0; A8Y5B4; A8Y5B5; Q24539; Q5BI40; Q6NKM3; Q7PLW9; Q7PLX0;
AC Q7PLX1; Q8MZI2; Q9W5P7;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Protein suppressor of forked;
GN Name=su(f); ORFNames=CG17170;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM E), FUNCTION, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=Oregon-R;
RX PubMed=8436295; DOI=10.1101/gad.7.2.241;
RA Mitchelson A., Simonelig M., Williams C., O'Hare K.;
RT "Homology with Saccharomyces cerevisiae RNA14 suggests that phenotypic
RT suppression in Drosophila melanogaster by suppressor of forked occurs at
RT the level of RNA stability.";
RL Genes Dev. 7:241-249(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM E).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-351 (ISOFORMS B/E).
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-738, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-738, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Essential protein, may play a role in mRNA production or
CC stability. {ECO:0000269|PubMed:8436295}.
CC -!- SUBUNIT: Probably interacts with an RNA-binding protein.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B;
CC IsoId=P25991-1; Sequence=Displayed;
CC Name=E;
CC IsoId=P25991-4; Sequence=VSP_023342;
CC -!- DEVELOPMENTAL STAGE: Present throughout development. Most abundant in
CC embryos, pupae and adult females. {ECO:0000269|PubMed:8436295}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM27493.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
CC Sequence=CAA44552.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X62679; CAA44551.1; -; Genomic_DNA.
DR EMBL; X62679; CAA44552.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AE014298; EAA46201.1; -; Genomic_DNA.
DR EMBL; AE014298; EDP28024.1; -; Genomic_DNA.
DR EMBL; AE014298; EDP28025.2; -; Genomic_DNA.
DR EMBL; BT012671; AAT09321.1; -; mRNA.
DR EMBL; BT021384; AAX33532.1; -; mRNA.
DR EMBL; AY102664; AAM27493.1; ALT_SEQ; mRNA.
DR PIR; A46389; A46389.
DR PIR; B46389; B46389.
DR RefSeq; NP_001015241.3; NM_001015241.4. [P25991-1]
DR RefSeq; NP_001104479.1; NM_001111009.4. [P25991-4]
DR RefSeq; NP_001104480.2; NM_001111010.3. [P25991-4]
DR AlphaFoldDB; P25991; -.
DR SMR; P25991; -.
DR BioGRID; 78119; 6.
DR DIP; DIP-22591N; -.
DR IntAct; P25991; 2.
DR STRING; 7227.FBpp0112455; -.
DR iPTMnet; P25991; -.
DR PaxDb; P25991; -.
DR PRIDE; P25991; -.
DR EnsemblMetazoa; FBtr0113733; FBpp0112456; FBgn0003559. [P25991-4]
DR EnsemblMetazoa; FBtr0306544; FBpp0297502; FBgn0003559. [P25991-1]
DR EnsemblMetazoa; FBtr0306545; FBpp0297503; FBgn0003559. [P25991-4]
DR GeneID; 3354994; -.
DR KEGG; dme:Dmel_CG17170; -.
DR UCSC; CG17170-RE; d. melanogaster.
DR CTD; 3354994; -.
DR FlyBase; FBgn0003559; su(f).
DR VEuPathDB; VectorBase:FBgn0003559; -.
DR eggNOG; KOG1914; Eukaryota.
DR GeneTree; ENSGT00390000006758; -.
DR InParanoid; P25991; -.
DR OMA; LCYIDYL; -.
DR PhylomeDB; P25991; -.
DR Reactome; R-DME-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DME-72187; mRNA 3'-end processing.
DR Reactome; R-DME-73856; RNA Polymerase II Transcription Termination.
DR Reactome; R-DME-77595; Processing of Intronless Pre-mRNAs.
DR BioGRID-ORCS; 3354994; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 3354994; -.
DR PRO; PR:P25991; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0003559; Expressed in eye disc (Drosophila) and 21 other tissues.
DR Genevisible; P25991; DM.
DR GO; GO:0005848; C:mRNA cleavage stimulating factor complex; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0031124; P:mRNA 3'-end processing; IEA:InterPro.
DR GO; GO:1903220; P:positive regulation of malate dehydrogenase (decarboxylating) (NADP+) activity; IMP:FlyBase.
DR GO; GO:0031123; P:RNA 3'-end processing; IBA:GO_Central.
DR InterPro; IPR003107; HAT.
DR InterPro; IPR045243; Rna14-like.
DR InterPro; IPR008847; Suf.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR19980; PTHR19980; 1.
DR Pfam; PF05843; Suf; 1.
DR SMART; SM00386; HAT; 10.
DR SUPFAM; SSF48452; SSF48452; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..765
FT /note="Protein suppressor of forked"
FT /id="PRO_0000205764"
FT REPEAT 46..78
FT /note="HAT 1"
FT REPEAT 80..111
FT /note="HAT 2"
FT REPEAT 118..153
FT /note="HAT 3"
FT REPEAT 164..197
FT /note="HAT 4"
FT REPEAT 230..262
FT /note="HAT 5"
FT REPEAT 387..420
FT /note="HAT 6"
FT REPEAT 491..527
FT /note="HAT 7"
FT REGION 595..615
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 727..747
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 738
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656,
FT ECO:0000269|PubMed:18327897"
FT VAR_SEQ 314..345
FT /note="Missing (in isoform E)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_023342"
FT CONFLICT 346..351
FT /note="DVQAAK -> VKTKNE (in Ref. 5; AAM27493)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 765 AA; 88214 MW; 824B61346110ECAB CRC64;
MSSARDLIKV DIEWGMERLV RAQQVVELRP YDIESWSVMI REAQTRPIHE VRSLYESLVN
VFPTTARYWK LYIEMEMRSR YYERVEKLFQ RCLVKILNID LWKLYLTYVK ETKSGLSTHK
EKMAQAYDFA LEKIGMDLHS FSIWQDYIYF LRGVEAVGNY AENQKITAVR RVYQKAVVTP
IVGIEQLWKD YIAFEQNINP IISEKMSLER SKDYMNARRV AKELEYHTKG LNRNLPAVPP
TLTKEEVKQV ELWKRFITYE KSNPLRTEDT ALVTRRVMFA TEQCLLVLTH HPAVWHQASQ
FLDTSARVLT EKGVRTSVEN ISPILCVPVV NQIEWVMAFA WWWAKDVQAA KIFADECANI
LERSINGVLN RNALLYFAYA DFEEGRLKYE KVHTMYNKLL QLPDIDPTLV YVQYMKFARR
AEGIKSARSI FKKAREDVRS RYHIFVAAAL MEYYCSKDKE IAFRIFELGL KRFGGSPEYV
MCYIDYLSHL NEDNNTRVLF ERVLSSGGLS PHKSVEVWNR FLEFESNIGD LSSIVKVERR
RSAVFENLKE YEGKETAQLV DRYKFLDLYP CTSTELKSIG YAENVGIILN KVGGGAQSQN
TGEVETDSEA TPPLPRPDFS QMIPFKPRPC AHPGAHPLAG GVFPQPPALA ALCATLPPPN
SFRGPFVSVE LLFDIFMRLN LPDSAPQPNG DNELSPKIFD LAKSVHWIVD TSTYTGVQHS
VTAVPPRRRR LLPGGDDSDD ELQTAVPPSH DIYRLRQLKR FAKSN
