SUGP1_MOUSE
ID SUGP1_MOUSE Reviewed; 643 AA.
AC Q8CH02; Q0VAT9; Q3U0W3; Q8R094;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=SURP and G-patch domain-containing protein 1;
DE AltName: Full=Splicing factor 4;
GN Name=Sugp1; Synonyms=Sf4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=12594045; DOI=10.1016/s0378-1119(02)01230-1;
RA Sampson N.D., Hewitt J.E.;
RT "SF4 and SFRS14, two related putative splicing factors on human chromosome
RT 19p13.11.";
RL Gene 305:91-100(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD; TISSUE=Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-483, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407; SER-409 AND SER-483, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP STRUCTURE BY NMR OF 165-239.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of SURP domain in BAB30904.";
RL Submitted (AUG-2004) to the PDB data bank.
RN [8]
RP STRUCTURE BY NMR OF 250-314.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of SURP domain in splicing factor 4.";
RL Submitted (NOV-2005) to the PDB data bank.
CC -!- FUNCTION: Plays a role in pre-mRNA splicing. {ECO:0000250}.
CC -!- SUBUNIT: Component of the spliceosome. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
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DR EMBL; AF521129; AAN77124.1; -; mRNA.
DR EMBL; AK156508; BAE33738.1; -; mRNA.
DR EMBL; BC120919; AAI20920.1; -; mRNA.
DR EMBL; BC120920; AAI20921.1; -; mRNA.
DR CCDS; CCDS22356.1; -.
DR RefSeq; NP_081757.1; NM_027481.2.
DR RefSeq; XP_006509801.1; XM_006509738.1.
DR PDB; 1UG0; NMR; -; A=165-239.
DR PDB; 1X4O; NMR; -; A=250-314.
DR PDBsum; 1UG0; -.
DR PDBsum; 1X4O; -.
DR AlphaFoldDB; Q8CH02; -.
DR SMR; Q8CH02; -.
DR BioGRID; 214168; 4.
DR IntAct; Q8CH02; 1.
DR STRING; 10090.ENSMUSP00000011450; -.
DR iPTMnet; Q8CH02; -.
DR PhosphoSitePlus; Q8CH02; -.
DR EPD; Q8CH02; -.
DR jPOST; Q8CH02; -.
DR MaxQB; Q8CH02; -.
DR PaxDb; Q8CH02; -.
DR PeptideAtlas; Q8CH02; -.
DR PRIDE; Q8CH02; -.
DR ProteomicsDB; 257374; -.
DR Antibodypedia; 1687; 164 antibodies from 27 providers.
DR DNASU; 70616; -.
DR Ensembl; ENSMUST00000011450; ENSMUSP00000011450; ENSMUSG00000011306.
DR GeneID; 70616; -.
DR KEGG; mmu:70616; -.
DR UCSC; uc009lyn.1; mouse.
DR CTD; 57794; -.
DR MGI; MGI:1917866; Sugp1.
DR VEuPathDB; HostDB:ENSMUSG00000011306; -.
DR eggNOG; KOG0965; Eukaryota.
DR GeneTree; ENSGT00410000025695; -.
DR HOGENOM; CLU_028403_0_0_1; -.
DR InParanoid; Q8CH02; -.
DR OMA; QWLEIKI; -.
DR OrthoDB; 1232201at2759; -.
DR PhylomeDB; Q8CH02; -.
DR TreeFam; TF326321; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR BioGRID-ORCS; 70616; 6 hits in 72 CRISPR screens.
DR EvolutionaryTrace; Q8CH02; -.
DR PRO; PR:Q8CH02; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8CH02; protein.
DR Bgee; ENSMUSG00000011306; Expressed in seminiferous tubule of testis and 249 other tissues.
DR Genevisible; Q8CH02; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.790; -; 2.
DR InterPro; IPR000467; G_patch_dom.
DR InterPro; IPR040169; SUGP1/2.
DR InterPro; IPR000061; Surp.
DR InterPro; IPR035967; SWAP/Surp_sf.
DR PANTHER; PTHR23340; PTHR23340; 1.
DR Pfam; PF01585; G-patch; 1.
DR Pfam; PF01805; Surp; 2.
DR SMART; SM00443; G_patch; 1.
DR SMART; SM00648; SWAP; 2.
DR SUPFAM; SSF109905; SSF109905; 2.
DR PROSITE; PS50174; G_PATCH; 1.
DR PROSITE; PS50128; SURP; 2.
PE 1: Evidence at protein level;
KW 3D-structure; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Spliceosome.
FT CHAIN 1..643
FT /note="SURP and G-patch domain-containing protein 1"
FT /id="PRO_0000097702"
FT REPEAT 187..229
FT /note="SURP motif 1"
FT REPEAT 262..305
FT /note="SURP motif 2"
FT DOMAIN 560..607
FT /note="G-patch"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00092"
FT REGION 48..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 97..119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 316..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 360..393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 378..384
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOD_RES 128
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q8IWZ8"
FT MOD_RES 252
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8IWZ8"
FT MOD_RES 322
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8IWZ8"
FT MOD_RES 407
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 409
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 412
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8IWZ8"
FT MOD_RES 483
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
FT CONFLICT 326
FT /note="P -> L (in Ref. 3; AAI20920/AAI20921)"
FT /evidence="ECO:0000305"
FT HELIX 167..172
FT /evidence="ECO:0007829|PDB:1UG0"
FT HELIX 179..181
FT /evidence="ECO:0007829|PDB:1UG0"
FT HELIX 182..198
FT /evidence="ECO:0007829|PDB:1UG0"
FT HELIX 200..209
FT /evidence="ECO:0007829|PDB:1UG0"
FT TURN 210..212
FT /evidence="ECO:0007829|PDB:1UG0"
FT STRAND 214..216
FT /evidence="ECO:0007829|PDB:1UG0"
FT HELIX 217..220
FT /evidence="ECO:0007829|PDB:1UG0"
FT HELIX 225..239
FT /evidence="ECO:0007829|PDB:1UG0"
FT HELIX 257..272
FT /evidence="ECO:0007829|PDB:1X4O"
FT HELIX 277..287
FT /evidence="ECO:0007829|PDB:1X4O"
FT TURN 291..295
FT /evidence="ECO:0007829|PDB:1X4O"
FT STRAND 297..300
FT /evidence="ECO:0007829|PDB:1X4O"
FT HELIX 301..313
FT /evidence="ECO:0007829|PDB:1X4O"
SQ SEQUENCE 643 AA; 72649 MW; A1FCD38A26998E78 CRC64;
MSLKMDNRDV AGKANRWFGM AQPKSGKMNM NILHQEELIA QKKREIEARM EQKARQSHVP
SPQPPHPGEI ADAHNSCISN KFANDGSFLQ QFLKLQKAQT STDSAPRAPP SMPTPSSLKK
PLVLSKRTGL GLSSPTGPVK NYSHAKQLPV AHRPSVFQSP DDDEEEDYEQ WLEIKVSPPE
GAETRRVIEK LARFVAEGGP ELEKVAMEDY KDNPAFTFLH DKNSREFLYY RRKVAEIRKE
AQKPQAATQK VSPPEDEEAK NLAEKLARFI ADGGPEVETI ALQNNRENQA FSFLYDPNSQ
GYRYYRQKLD EFRKAKAGST GSFPAPAPNP SLRRKSAPEA LSGAVPPITA CPTPVAPAPA
VNPTPSIPGK PTATAAVKRK RKSRWGPEED KVELPPAELA QRDIDASPSP LSVQDLKGLG
YEKGKPVGLV GVTELSDAQK KQLKEQQEMQ QMYDMIMQHK RAMQDMQLLW EKALQQHQHG
YDSDEEVDSE LGTWEHQLRR MEMDKTREWA EQLTQMGRGK HFIGDFLPPD ELEKFMETFK
ALKEGREPDY SEYKEFKLTV ENIGYQMLMK MGWKEGEGLG TEGQGIKNPV NKGATTIDGA
GFGIDRPAEL SKEDDEYEAF RKRMMLAYRF RPNPLNNPRR PYY
